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Article

Mesenchymal stem cells reverse high‑fat diet‑induced non‑alcoholic fatty liver disease through suppression of CD4+ T lymphocytes in mice

  • Authors:
    • Huafeng Wang
    • Huan Zhang
    • Biao Huang
    • Guolin Miao
    • Xiaoyan Yan
    • Gang Gao
    • Yongping Luo
    • Huize Chen
    • Wei Chen
    • Luhong Yang
  • View Affiliations / Copyright

    Affiliations: Modern College of Arts and Science and School of Life Science, Shanxi Normal University, Linfen, Shanxi 041004, P.R. China, Clinical Laboratory, Tianjin Academy of Traditional Chinese Medicine Affiliated Hospital, Tianjin 300120, P.R. China, Research Center of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, P.R. China
  • Pages: 3769-3774
    |
    Published online on: December 20, 2017
       https://doi.org/10.3892/mmr.2017.8326
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Abstract

Although the multipotency of mesenchymal stem cells (MSCs) makes them an attractive choice for clinical applications, immune modulation is an important factor affecting MSC transplantation. At present, the effect of treatment with MSCs on non‑alcoholic fatty liver disease (NAFLD) has received little attention. In the present study, a compact bone‑derived method was used to isolate mouse MSCs (mMSCs) and a high‑fat diet was used to establish a mouse model of NAFLD. Immunophenotypic features of mMSCs were analyzed using flow cytometry. Paraffin sections were stained with hematoxylin and eosin to assess inflammation and steatosis, and with picrosirius red to assess fibrosis. Spleen leukocytes were analyzed by flow cytometry. The results demonstrated that compact bone‑derived MSC transplantation decreased high‑fat diet‑induced weight gain, expansion of subcutaneous adipose tissue, steatosis, lobular inflammation and liver fibrogenesis. Flow cytometry analysis of spleen leukocytes demonstrated that compact bone‑derived MSC transplantation suppressed the proliferation of cluster of differentiation (CD) 4+ T lymphocytes in the spleen, which had been induced by the high‑fat diet. In conclusion, compact bone‑derived MSCs may exhibit clinical value in the treatment of NAFLD through their capacity to suppress the activation of CD4+ T cells.
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1 

Lieber CS, Leo MA, Mak KM, Xu Y, Cao Q, Ren C, Ponomarenko A and DeCarli LM: Model of nonalcoholic steatohepatitis. Am J Clin Nutr. 79:502–509. 2004. View Article : Google Scholar : PubMed/NCBI

2 

Flegal KM, Carroll MD, Kit BK and Ogden CL: Prevalence of obesity and trends in the distribution of body mass index among US adults, 1999–2010. Jama. 307:491–497. 2012. View Article : Google Scholar : PubMed/NCBI

3 

Ahmed M: Non-alcoholic fatty liver disease in 2015. World J Hepatol. 7:1450–1459. 2015. View Article : Google Scholar : PubMed/NCBI

4 

Wong VW: Nonalcoholic fatty liver disease in Asia: A story of growth. J Gastroenterol Hepatol. 28:18–23. 2013. View Article : Google Scholar : PubMed/NCBI

5 

Fan J: Steatohepatitis studies in China. World Chi J Digestology. 9:6–10. 2001.

6 

Fan JG and Farrell GC: Epidemiology of non-alcoholic fatty liver disease in China. J Hepatol. 50:204–210. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Zhang HJ, Zhuang H and Liu XE: Advances in the epidemiological study of fatty liver. Zhonghua Liu Xing Bing Xue Za Zhi. 25:630–632. 2004.(In Chinese). PubMed/NCBI

8 

Wang H, Zhang H, Zhang Z, Huang B, Cheng X, Wang D, La Gahu Z, Xue Z, Da Y, Li D, et al: Adiponectin-derived active peptide ADP355 exerts anti-inflammatory and anti-fibrotic activities in thioacetamide-induced liver injury. Sci Rep. 6:194452016. View Article : Google Scholar : PubMed/NCBI

9 

Uccelli A, Moretta L and Pistoia V: Mesenchymal stem cells in health and disease. Nat Rev Immunol. 8:726–736. 2008. View Article : Google Scholar : PubMed/NCBI

10 

Ding DC, Shyu WC and Lin SZ: Mesenchymal stem cells. Cell Transplant. 20:5–14. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Huang B, Li G and Jiang XH: Fate determination in mesenchymal stem cells: A perspective from histone-modifying enzymes. Stem Cell Res Ther. 6:352015. View Article : Google Scholar : PubMed/NCBI

12 

Ke C, Biao H, Qianqian L, Yunwei S and Xiaohua J: Mesenchymal stem cell therapy for inflammatory bowel diseases: Promise and challenge. Curr Stem Cell Res Ther. 10:499–508. 2015. View Article : Google Scholar : PubMed/NCBI

13 

Rankin S: Mesenchymal stem cells. Thorax. 67:565–566. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Sato Y, Araki H, Kato J, Nakamura K, Kawano Y, Kobune M, Sato T, Miyanishi K, Takayama T, Takahashi M, et al: Human mesenchymal stem cells xenografted directly to rat liver are differentiated into human hepatocytes without fusion. Blood. 106:756–763. 2005. View Article : Google Scholar : PubMed/NCBI

15 

Fang B, Shi M, Liao L, Yang S, Liu Y and Zhao RC: Systemic infusion of FLK1(+) mesenchymal stem cells ameliorate carbon tetrachloride-induced liver fibrosis in mice. Transplantation. 78:83–88. 2004. View Article : Google Scholar : PubMed/NCBI

16 

Aurich I, Mueller LP, Aurich H, Luetzkendorf J, Tisljar K, Dollinger MM, Schormann W, Walldorf J, Hengstler JG, Fleig WE and Christ B: Functional integration of hepatocytes derived from human mesenchymal stem cells into mouse livers. Gut. 56:405–415. 2007. View Article : Google Scholar : PubMed/NCBI

17 

Parekkadan B, Van Poll D, Suganuma K, Carter EA, Berthiaume F, Tilles AW and Yarmush ML: Mesenchymal stem cell-derived molecules reverse fulminant hepatic failure. PLoS One. 2:e9412007. View Article : Google Scholar : PubMed/NCBI

18 

Kuo TK, Hung SP, Chuang CH, Chen CT, Shih YR, Fang SC, Yang VW and Lee OK: Stem cell therapy for liver disease: Parameters governing the success of using bone marrow mesenchymal stem cells. Gastroenterology. 134:2111–2121, e1-e3. 2008. View Article : Google Scholar : PubMed/NCBI

19 

Huang B, Cheng X, Wang H, Huang W, La Ga Hu Z, Wang D, Zhang K, Zhang H, Xue Z, Da Y, et al: Mesenchymal stem cells and their secreted molecules predominantly ameliorate fulminant hepatic failure and chronic liver fibrosis in mice respectively. J Transl Med. 14:452016. View Article : Google Scholar : PubMed/NCBI

20 

Liu XJ, Wang BW, Zhang C, Xia MZ, Chen YH, Hu CQ, Wang H, Chen X and Xu DX: Vitamin d deficiency attenuates high-fat diet-induced hyperinsulinemia and hepatic lipid accumulation in male mice. Endocrinology. 156:2103–2113. 2015. View Article : Google Scholar : PubMed/NCBI

21 

Zhu H, Guo ZK, Jiang XX, Li H, Wang XY, Yao HY, Zhang Y and Mao N: A protocol for isolation and culture of mesenchymal stem cells from mouse compact bone. Nat Protoc. 5:550–560. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Friedenstein AJ, Gorskaja J and Kulagina NN: Fibroblast precursors in normal and irradiated mouse hematopoietic organs. Exp Hematol. 4:267–274. 1976.PubMed/NCBI

23 

Phinney DG, Kopen G, Isaacson RL and Prockop DJ: Plastic adherent stromal cells from the bone marrow of commonly used strains of inbred mice: Variations in yield, growth, and differentiation. J Cell Biochem. 72:570–585. 1999. View Article : Google Scholar : PubMed/NCBI

24 

Meier RP, Müller YD, Morel P, Gonelle-Gispert C and Bühler LH: Transplantation of mesenchymal stem cells for the treatment of liver diseases, is there enough evidence? Stem Cell Res. 11:1348–1364. 2013. View Article : Google Scholar : PubMed/NCBI

25 

Di Nicola M, Carlo-Stella C, Magni M, Milanesi M, Longoni PD, Matteucci P, Grisanti S and Gianni AM: Human bone marrow stromal cells suppress T-lymphocyte proliferation induced by cellular or nonspecific mitogenic stimuli. Blood. 99:3838–3843. 2002. View Article : Google Scholar : PubMed/NCBI

26 

Zappia E, Casazza S, Pedemonte E, Benvenuto F, Bonanni I, Gerdoni E, Giunti D, Ceravolo A, Cazzanti F, Frassoni F, et al: Mesenchymal stem cells ameliorate experimental autoimmune encephalomyelitis inducing T-cell anergy. Blood. 106:1755–1761. 2005. View Article : Google Scholar : PubMed/NCBI

27 

Augello A, Tasso R, Negrini SM, Cancedda R and Pennesi G: Cell therapy using allogeneic bone marrow mesenchymal stem cells prevents tissue damage in collagen-induced arthritis. Arthritis Rheum. 56:1175–1186. 2007. View Article : Google Scholar : PubMed/NCBI

28 

Urbán VS, Kiss J, Kovács J, Gócza E, Vas V, Monostori E and Uher F: Mesenchymal stem cells cooperate with bone marrow cells in therapy of diabetes. Stem Cells. 26:244–253. 2008. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang H, Zhang H, Huang B, Miao G, Yan X, Gao G, Luo Y, Chen H, Chen W, Yang L, Yang L, et al: Mesenchymal stem cells reverse high‑fat diet‑induced non‑alcoholic fatty liver disease through suppression of CD4+ T lymphocytes in mice. Mol Med Rep 17: 3769-3774, 2018.
APA
Wang, H., Zhang, H., Huang, B., Miao, G., Yan, X., Gao, G. ... Yang, L. (2018). Mesenchymal stem cells reverse high‑fat diet‑induced non‑alcoholic fatty liver disease through suppression of CD4+ T lymphocytes in mice. Molecular Medicine Reports, 17, 3769-3774. https://doi.org/10.3892/mmr.2017.8326
MLA
Wang, H., Zhang, H., Huang, B., Miao, G., Yan, X., Gao, G., Luo, Y., Chen, H., Chen, W., Yang, L."Mesenchymal stem cells reverse high‑fat diet‑induced non‑alcoholic fatty liver disease through suppression of CD4+ T lymphocytes in mice". Molecular Medicine Reports 17.3 (2018): 3769-3774.
Chicago
Wang, H., Zhang, H., Huang, B., Miao, G., Yan, X., Gao, G., Luo, Y., Chen, H., Chen, W., Yang, L."Mesenchymal stem cells reverse high‑fat diet‑induced non‑alcoholic fatty liver disease through suppression of CD4+ T lymphocytes in mice". Molecular Medicine Reports 17, no. 3 (2018): 3769-3774. https://doi.org/10.3892/mmr.2017.8326
Copy and paste a formatted citation
x
Spandidos Publications style
Wang H, Zhang H, Huang B, Miao G, Yan X, Gao G, Luo Y, Chen H, Chen W, Yang L, Yang L, et al: Mesenchymal stem cells reverse high‑fat diet‑induced non‑alcoholic fatty liver disease through suppression of CD4+ T lymphocytes in mice. Mol Med Rep 17: 3769-3774, 2018.
APA
Wang, H., Zhang, H., Huang, B., Miao, G., Yan, X., Gao, G. ... Yang, L. (2018). Mesenchymal stem cells reverse high‑fat diet‑induced non‑alcoholic fatty liver disease through suppression of CD4+ T lymphocytes in mice. Molecular Medicine Reports, 17, 3769-3774. https://doi.org/10.3892/mmr.2017.8326
MLA
Wang, H., Zhang, H., Huang, B., Miao, G., Yan, X., Gao, G., Luo, Y., Chen, H., Chen, W., Yang, L."Mesenchymal stem cells reverse high‑fat diet‑induced non‑alcoholic fatty liver disease through suppression of CD4+ T lymphocytes in mice". Molecular Medicine Reports 17.3 (2018): 3769-3774.
Chicago
Wang, H., Zhang, H., Huang, B., Miao, G., Yan, X., Gao, G., Luo, Y., Chen, H., Chen, W., Yang, L."Mesenchymal stem cells reverse high‑fat diet‑induced non‑alcoholic fatty liver disease through suppression of CD4+ T lymphocytes in mice". Molecular Medicine Reports 17, no. 3 (2018): 3769-3774. https://doi.org/10.3892/mmr.2017.8326
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