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Association between ACE2/ACE balance and pneumocyte apoptosis in a porcine model of acute pulmonary thromboembolism with cardiac arrest

  • Authors:
    • Hong‑Li Xiao
    • Lian‑Xing Zhao
    • Jun Yang
    • Nan Tong
    • Le An
    • Qi‑Tong Liu
    • Miao‑Rong Xie
    • Chun‑Sheng Li
  • View Affiliations / Copyright

    Affiliations: Department of Emergency Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, P.R. China, Beijing Key Laboratory of Cardiopulmonary Cerebral Resuscitation, Beijing Chao‑Yang Hospital, Capital Medical University, Beijing 100020, P.R. China, Department of Radiology, Beijing Chao‑Yang Hospital, Capital Medical University, Beijing 100020, P.R. China
    Copyright: © Xiao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4221-4228
    |
    Published online on: January 12, 2018
       https://doi.org/10.3892/mmr.2018.8426
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Abstract

Acute pulmonary embolism (APE) is frequently reported in patients with cardiac arrest (CA) in emergency care. Pneumocyte apoptosis is commonly observed in the lungs following an APE. An important pathological mechanism evoking apoptosis during a lipopolysaccharide‑induced acute lung injury is the angiotensin‑converting enzyme 2 (ACE2)/ACE imbalance. The present study uses a porcine model to examine the anti‑apoptotic effects of captopril on APE‑CA and the return of spontaneous circulation (ROSC). Pigs were randomly assigned into four groups: Control, APE‑CA, ROSC‑saline, and ROSC‑captopril. Surviving pigs were euthanized at 6 h and lungs were isolated for analysis using several biochemical assays. Compared with the control group, the ACE2/ACE ratio was lower in the APE‑CA and ROSC pigs. In addition, APE‑CA pigs had higher Bcl‑2‑associated X protein (Bax) and cleaved caspase‑3 levels, and lower B‑cell lymphoma‑2 (Bcl‑2) level compared to control pigs. Captopril treatment reduced lung apoptosis, as demonstrated by lower TUNEL‑positive cells, higher Bcl‑2, and lower cleaved caspase‑3 protein levels in the lung. Notably, the ACE2/ACE ratio was positively correlated with Bcl‑2 protein levels and Bcl‑2/Bax ratio. In conclusion, captopril has a protective effect against lung apoptosis following ROSC and that maintaining the balance of the ACE2/ACE axis is important for inhibiting pulmonary apoptosis during APE.
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Copy and paste a formatted citation
Spandidos Publications style
Xiao HL, Zhao LX, Yang J, Tong N, An L, Liu QT, Xie MR and Li CS: Association between ACE2/ACE balance and pneumocyte apoptosis in a porcine model of acute pulmonary thromboembolism with cardiac arrest. Mol Med Rep 17: 4221-4228, 2018.
APA
Xiao, H., Zhao, L., Yang, J., Tong, N., An, L., Liu, Q. ... Li, C. (2018). Association between ACE2/ACE balance and pneumocyte apoptosis in a porcine model of acute pulmonary thromboembolism with cardiac arrest. Molecular Medicine Reports, 17, 4221-4228. https://doi.org/10.3892/mmr.2018.8426
MLA
Xiao, H., Zhao, L., Yang, J., Tong, N., An, L., Liu, Q., Xie, M., Li, C."Association between ACE2/ACE balance and pneumocyte apoptosis in a porcine model of acute pulmonary thromboembolism with cardiac arrest". Molecular Medicine Reports 17.3 (2018): 4221-4228.
Chicago
Xiao, H., Zhao, L., Yang, J., Tong, N., An, L., Liu, Q., Xie, M., Li, C."Association between ACE2/ACE balance and pneumocyte apoptosis in a porcine model of acute pulmonary thromboembolism with cardiac arrest". Molecular Medicine Reports 17, no. 3 (2018): 4221-4228. https://doi.org/10.3892/mmr.2018.8426
Copy and paste a formatted citation
x
Spandidos Publications style
Xiao HL, Zhao LX, Yang J, Tong N, An L, Liu QT, Xie MR and Li CS: Association between ACE2/ACE balance and pneumocyte apoptosis in a porcine model of acute pulmonary thromboembolism with cardiac arrest. Mol Med Rep 17: 4221-4228, 2018.
APA
Xiao, H., Zhao, L., Yang, J., Tong, N., An, L., Liu, Q. ... Li, C. (2018). Association between ACE2/ACE balance and pneumocyte apoptosis in a porcine model of acute pulmonary thromboembolism with cardiac arrest. Molecular Medicine Reports, 17, 4221-4228. https://doi.org/10.3892/mmr.2018.8426
MLA
Xiao, H., Zhao, L., Yang, J., Tong, N., An, L., Liu, Q., Xie, M., Li, C."Association between ACE2/ACE balance and pneumocyte apoptosis in a porcine model of acute pulmonary thromboembolism with cardiac arrest". Molecular Medicine Reports 17.3 (2018): 4221-4228.
Chicago
Xiao, H., Zhao, L., Yang, J., Tong, N., An, L., Liu, Q., Xie, M., Li, C."Association between ACE2/ACE balance and pneumocyte apoptosis in a porcine model of acute pulmonary thromboembolism with cardiac arrest". Molecular Medicine Reports 17, no. 3 (2018): 4221-4228. https://doi.org/10.3892/mmr.2018.8426
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