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Article

Combination of Runx2 and Cbfβ upregulates Amelotin gene expression in ameloblasts by directly interacting with cis‑enhancers during amelogenesis

  • Authors:
    • Xiaoying Liu
    • Yumin Wang
    • Li Zhang
    • Zhenzhen Xu
    • Qing Chu
    • Chang Xu
    • Yan Sun
    • Yuguang Gao
  • View Affiliations / Copyright

    Affiliations: Department of Oral Biology, Weifang Medical University, Weifang, Shandong 261053, P.R. China, Department of Pediatric Dentistry, Binzhou Medical University, Yantai, Shandong 264003, P.R. China
  • Pages: 6068-6076
    |
    Published online on: February 6, 2018
       https://doi.org/10.3892/mmr.2018.8564
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Abstract

Amelotin (Amtn) is a recently identified enamel protein secreted by ameloblasts at late stage of enamel development. Runt‑related transcription factor 2 (Runx2) in combination with the coactivator core‑binding factor β (Cbfβ) regulates the early stages of tooth development. The aim of the present study was to investigate the role of Runx2 in the regulation of Amtn gene expression in ameloblasts. Immunohistochemistry was performed and the results revealed that Runx2 protein was predominantly expressed in the nuclei of ameloblasts during the transition stage and the maturation stage of enamel development, whereas Cbfβ was expressed in ameloblasts from the secretory stage to the maturation stage. Reverse transcription‑quantitative polymerase chain reaction results demonstrated that Runx2 knockdown decreased Amtn expression in ameloblast‑lineage cells and co‑expression of Runx2 and Cbfβ in ameloblast lineage cells induced an upregulation in Amtn gene expression. Two putative Runx2‑binding sites within the Amtn promoter were identified using bioinformatics analysis. Results of an electrophoretic mobility shift assay and chromatin immunoprecipitation indicated that Runx2/Cbfβ bound to specific DNA sequences. Site‑directed mutagenesis of the Runx2 binding sites within the Amtn promoter resulted in decreased basal promoter activity and did not affect the overexpressed Runx2/Cbfβ. The results of the present study suggest that Runx2 upregulates Amtn gene expression via binding directly to Runx2 sites within the Amtn promoter during amelogenesis.
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Copy and paste a formatted citation
Spandidos Publications style
Liu X, Wang Y, Zhang L, Xu Z, Chu Q, Xu C, Sun Y and Gao Y: Combination of Runx2 and Cbfβ upregulates Amelotin gene expression in ameloblasts by directly interacting with cis‑enhancers during amelogenesis. Mol Med Rep 17: 6068-6076, 2018.
APA
Liu, X., Wang, Y., Zhang, L., Xu, Z., Chu, Q., Xu, C. ... Gao, Y. (2018). Combination of Runx2 and Cbfβ upregulates Amelotin gene expression in ameloblasts by directly interacting with cis‑enhancers during amelogenesis. Molecular Medicine Reports, 17, 6068-6076. https://doi.org/10.3892/mmr.2018.8564
MLA
Liu, X., Wang, Y., Zhang, L., Xu, Z., Chu, Q., Xu, C., Sun, Y., Gao, Y."Combination of Runx2 and Cbfβ upregulates Amelotin gene expression in ameloblasts by directly interacting with cis‑enhancers during amelogenesis". Molecular Medicine Reports 17.4 (2018): 6068-6076.
Chicago
Liu, X., Wang, Y., Zhang, L., Xu, Z., Chu, Q., Xu, C., Sun, Y., Gao, Y."Combination of Runx2 and Cbfβ upregulates Amelotin gene expression in ameloblasts by directly interacting with cis‑enhancers during amelogenesis". Molecular Medicine Reports 17, no. 4 (2018): 6068-6076. https://doi.org/10.3892/mmr.2018.8564
Copy and paste a formatted citation
x
Spandidos Publications style
Liu X, Wang Y, Zhang L, Xu Z, Chu Q, Xu C, Sun Y and Gao Y: Combination of Runx2 and Cbfβ upregulates Amelotin gene expression in ameloblasts by directly interacting with cis‑enhancers during amelogenesis. Mol Med Rep 17: 6068-6076, 2018.
APA
Liu, X., Wang, Y., Zhang, L., Xu, Z., Chu, Q., Xu, C. ... Gao, Y. (2018). Combination of Runx2 and Cbfβ upregulates Amelotin gene expression in ameloblasts by directly interacting with cis‑enhancers during amelogenesis. Molecular Medicine Reports, 17, 6068-6076. https://doi.org/10.3892/mmr.2018.8564
MLA
Liu, X., Wang, Y., Zhang, L., Xu, Z., Chu, Q., Xu, C., Sun, Y., Gao, Y."Combination of Runx2 and Cbfβ upregulates Amelotin gene expression in ameloblasts by directly interacting with cis‑enhancers during amelogenesis". Molecular Medicine Reports 17.4 (2018): 6068-6076.
Chicago
Liu, X., Wang, Y., Zhang, L., Xu, Z., Chu, Q., Xu, C., Sun, Y., Gao, Y."Combination of Runx2 and Cbfβ upregulates Amelotin gene expression in ameloblasts by directly interacting with cis‑enhancers during amelogenesis". Molecular Medicine Reports 17, no. 4 (2018): 6068-6076. https://doi.org/10.3892/mmr.2018.8564
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