Phenformin inhibits cell proliferation and induces cell apoptosis and autophagy in cholangiocarcinoma

Hu,Shuyang; Ouyang,Qing; Cheng,Qingbao; Wang,Jinghan; Feng,Feiling; Qiao,Liang; Gan,Wei; Shi,Yang; Wu,Demin; Jiang,Xiaoqing

doi:10.3892/mmr.2018.8573

April-2018 Volume 17 Issue 4

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April-2018 Volume 17 Issue 4

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Article

Phenformin inhibits cell proliferation and induces cell apoptosis and autophagy in cholangiocarcinoma

Authors:
- Shuyang Hu
- Qing Ouyang
- Qingbao Cheng
- Jinghan Wang
- Feiling Feng
- Liang Qiao
- Wei Gan
- Yang Shi
- Demin Wu
- Xiaoqing Jiang
View Affiliations / Copyright

Affiliations: First Department of Biliary Surgery, Eastern Hepatobiliary Surgical Hospital, The Second Military Medical University, Shanghai 200438, P.R. China
Pages: 6028-6032
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Published online on: February 8, 2018

https://doi.org/10.3892/mmr.2018.8573
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Abstract

Cholangiocarcinoma (CCA) is an aggressive malignant tumor and the prognosis of patients with advanced stage disease remains poor. Therefore, the identification of novel treatment agents for CCA is required. In the present study, the biological effects of the diabetes therapeutic agent, phenformin, in CCA cell lines was investigated. Cell Counting Kit‑8 cell viability, cellular clone formation and subcutaneous tumor formation assays were performed, which revealed that phenformin inhibited CCA cell proliferation and growth both in vitro and in vivo. In addition, phenformin induced CCA cell apoptosis and autophagy. Phenformin partly activated the liver kinase B1 (LKB1)/5' AMP‑activated protein kinase signaling pathway to exert its biological effects on CCA cell lines, as demonstrated by knockdown of LKB1, which reversed these effects. In conclusion, the present study demonstrated the biological effects of phenformin in CCA and suggested that phenformin may be a potential novel agent for CCA treatment.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Phenformin inhibits cell proliferation and induces cell apoptosis and autophagy in cholangiocarcinoma

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