Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
April-2018 Volume 17 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
April-2018 Volume 17 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Spontaneous adipogenic differentiation potential of adipose‑derived stem cells decreased with increasing cell passages

  • Authors:
    • Duo Yang
    • Na Li
    • Guoying Zhang
  • View Affiliations / Copyright

    Affiliations: Central Laboratory, Beijing Shijitan Hospital of Capital Medical University, Beijing 100038, P.R. China
  • Pages: 6109-6115
    |
    Published online on: February 16, 2018
       https://doi.org/10.3892/mmr.2018.8619
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Primary adipose-derived stem cells (ADSCs) are a mixture of cell types including preadipocytes having the ability to spontaneously differentiate into adipocytes. The aim of the present study was to compare the spontaneous adipogenic differentiation potential of ADSCs at different passages to determine whether it decreased with continuous cell passages. Mouse ADSCs (mADSCs) were harvested and cells from passages 1 to 5 were used for experiments. The proliferation of mADSCs at different passages was tested using the cell counting kit‑8 assay. Reverse transcription‑quantitative polymerase chain reaction was used to determine relative mRNA expression levels of CCAAT/enhancer binding protein α (Cebpa and C/EBPα) and peroxisome proliferator‑activated receptor γ (Pparg and PPARγ), and western blot analysis was used to investigate C/EBPα and PPARγ protein expression. The cells were cultured using DMEM. The fixed cells were then stained using Oil Red O on days 14 and 28, and the obtained extracted dye was monitored for absorbance. The 510 nm absorbance from passages 1 to 5 was observed to be statistically different. The relative expression levels of Cebpa and Pparg for mADSCs from passage 1 were significantly higher when compared with those for mADSCs from passages 2 to 5 on days 3, 5 and 7. However, no difference was identified in the expression levels of proteins C/EBPα and PPARγ for different passages. Although the mRNA expression levels of Pparg from passages 4 to 5 were significantly higher when compared with those from passage 1, the results of Oil Red O absorbance, mRNA expression levels of Cebpa, and the protein expression levels of C/EBPα and PPARγ exhibited no difference between mADSCs from passages 1 to 5 when cultured with induced adipogenic differentiation medium. Therefore, it was concluded that the spontaneous adipogenic differentiation potential of ADSCs decreased with continuous cell passages.
View Figures

Figure 1

Figure 2

Figure 3

View References

1 

Rodeheffer MS, Birsoy K and Friedman JM: Identification of white adipocyte progenitor cells in vivo. Cell. 135:240–249. 2008. View Article : Google Scholar : PubMed/NCBI

2 

Yoshimura K, Shigeura T, Matsumoto D, Sato T, Takaki Y, Aiba-Kojima E, Sato K, Inoue K, Nagase T, Koshima I, et al: Characterization of freshly isolated and cultured cells derived from the fatty and fluid portions of liposuction aspirates. J Cell Physiol. 208:64–76. 2006. View Article : Google Scholar : PubMed/NCBI

3 

Bunnell BA, Flaat M, Gagliardi C, Patel B and Ripoll C: Adipose-derived stem cells: Isolation, expansion and differentiation. Methods. 45:115–120. 2008. View Article : Google Scholar : PubMed/NCBI

4 

Gimble JM, Guilak F and Bunnell BA: Clinical and preclinical translation of cell-based therapies using adipose tissue-derived cells. Stem Cell Res Ther. 1:192010. View Article : Google Scholar : PubMed/NCBI

5 

Gir P, Oni G, Brown SA, Mojallal A and Rohrich RJ: Human adipose stem cells: Current clinical applications. Plast Reconstr Surg. 129:1277–1290. 2012. View Article : Google Scholar : PubMed/NCBI

6 

Harasymiak-Krzyżanowska I, Niedojadło A, Karwat J, Kotula L, Gil-Kulik P, Sawiuk M and Kocki J: Adipose tissue-derived stem cells show considerable promise for regenerative medicine applications. Cell Mol Biol Lett. 18:479–493. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Tobita M, Orbay H and Mizuno H: Adipose-derived stem cells: Current findings and future perspectives. Discov Med. 11:160–170. 2011.PubMed/NCBI

8 

Romagnoli C and Brandi ML: Adipose mesenchymal stem cells in the field of bone tissue engineering. World J Stem Cells. 6:144–152. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Roxburgh J, Metcalfe AD and Martin YH: The effect of medium selection on adipose-derived stem cell expansion and differentiation: Implications for application in regenerative medicine. Cytotechnology. 68:957–967. 2016. View Article : Google Scholar : PubMed/NCBI

10 

Baer PC and Geiger H: Adipose-derived mesenchymal stromal/stem cells: Tissue localization, characterization, and heterogeneity. Stem Cells Int. 2012:8126932012. View Article : Google Scholar : PubMed/NCBI

11 

Locke M, Windsor J and Dunbar PR: Human adipose-derived stem cells: Isolation, characterization and applications in surgery. ANZ J Surg. 79:235–244. 2009. View Article : Google Scholar : PubMed/NCBI

12 

Fu Y, Deng J, Jiang Q, Wang Y, Zhang Y, Yao Y, Cheng F, Chen X, Xu F, Huang M, et al: Rapid generation of functional hepatocyte-like cells from human adipose-derived stem cells. Stem Cell Res Ther. 7:1052016. View Article : Google Scholar : PubMed/NCBI

13 

Orbay H, Devi K, Williams PA, Dehghani T, Silva EA and Sahar DE: Comparison of endothelial differentiation capacities of human and rat adipose-derived stem cells. Plast Reconstr Surg. 138:1231–1241. 2016. View Article : Google Scholar : PubMed/NCBI

14 

Dufrane D and Lafosse A: A simple method to determine the purity of adipose-derived stem cell-based cell therapies. Stem Cells Transl Med. 5:1575–1579. 2016. View Article : Google Scholar : PubMed/NCBI

15 

Lin YC, Harn HJ, Lin PC, Chuang MH, Chen CH, Lin SZ and Chiou TW: Commercial production of autologous stem cells and their therapeutic potential for liver cirrhosis. Cell Transplant. 26:449–460. 2017. View Article : Google Scholar : PubMed/NCBI

16 

Krähenbühl SM, Grognuz A, Michetti M, Raffoul W and Applegate LA: Enhancement of human adipose-derived stem cell expansion and stability for clinical use. Int J Stem Cell Res Ther. 2:72015.

17 

Liu Y, Zhang Z, Zhang C, Deng W, Lv Q, Chen X, Huang T and Pan L: Adipose-derived stem cells undergo spontaneous osteogenic differentiation in vitro when passaged serially or seeded at low density. Biotech Histochem. 91:369–376. 2016. View Article : Google Scholar : PubMed/NCBI

18 

Lowe CE, O'Rahilly S and Rochford JJ: Adipogenesis at a glance. J Cell Sci. 124:2681–2686. 2011. View Article : Google Scholar : PubMed/NCBI

19 

Zhang LJ, Guerrero-Juarez CF, Hata T, Bapat SP, Ramos R, Plikus MV and Gallo RL: Innate immunity. Dermal adipocytes protect against invasive Staphylococcus aureus skin infection. Science. 347:67–71. 2015. View Article : Google Scholar : PubMed/NCBI

20 

Muto J, Morioka Y, Yamasaki K, Kim M, Garcia A, Carlin AF, Varki A and Gallo RL: Hyaluronan digestion controls DC migration from the skin. J Clin Invest. 124:1309–1319. 2014. View Article : Google Scholar : PubMed/NCBI

21 

Zuk PA, Zhu M, Ashjian P, De Ugarte DA, Huang JI, Mizuno H, Alfonso ZC, Fraser JK, Benhaim P and Hedrick MH: Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell. 13:4279–4295. 2002. View Article : Google Scholar : PubMed/NCBI

22 

Ramirez-Zacarías JL, Castro-Muñozledo F and Kuri-Harcuch W: Quantitation of adipose conversion and triglycerides by staining intracytoplasmic lipids with Oil Red O. Histochemistry. 97:493–497. 1992. View Article : Google Scholar : PubMed/NCBI

23 

Bajek A, Gurtowska N, Olkowska J, Kazmierski L, Maj M and Drewa T: Adipose-derived stem cells as a tool in cell-based therapies. Arch Immunol Ther Exp (Warsz). 64:443–454. 2016. View Article : Google Scholar : PubMed/NCBI

24 

de Witte SF, Lambert EE, Merino A, Strini T, Douben HJ, O'Flynn L, Elliman SJ, de Klein AJ, Newsome PN, Baan CC, et al: Aging of bone marrow- and umbilical cord-derived mesenchymal stromal cells during expansion. Cytotherapy. 19:798–807. 2017. View Article : Google Scholar : PubMed/NCBI

25 

Bajek A, Gurtowska N, Olkowska J, Maj M, Kaźmierski Ł, Bodnar M, Marszałek A, Dębski R and Drewa T: Does the harvesting technique affect the properties of adipose-derived stem cells?-the comparative biological characterization. J Cell Biochem. 118:1097–1107. 2017. View Article : Google Scholar : PubMed/NCBI

26 

González-Cruz RD and Darling EM: Adipose-derived stem cell fate is predicted by cellular mechanical properties. Adipocyte. 2:87–91. 2013. View Article : Google Scholar : PubMed/NCBI

27 

Wang QF, Huang Y, He GC, Wang HS, Chen ZH, Cai XH, Xie YH and Liu Q: Osteoblast differentiation of rabbit adipose-derived stem cells by polyethylenimine-mediated BMP-2 gene transfection in vitro. Genet Mol Res. 16:2017. View Article : Google Scholar

28 

Aoyagi Y, Kuroda M, Asada S, Bujo H, Tanaka S, Konno S, Tanio M, Ishii I, Aso M and Saito Y: Fibrin glue increases the cell survival and the transduced gene product secretion of the ceiling culture-derived adipocytes transplanted in mice. Exp Mol Med. 43:161–167. 2011. View Article : Google Scholar : PubMed/NCBI

29 

Güven S, Mehrkens A, Saxer F, Schaefer DJ, Martinetti R, Martin I and Scherberich A: Engineering of large osteogenic grafts with rapid engraftment capacity using mesenchymal and endothelial progenitors from human adipose tissue. Biomaterials. 32:5801–5809. 2011. View Article : Google Scholar : PubMed/NCBI

30 

Jang Y, Jung H, Nam Y, Rim YA, Kim J, Jeong SH and Ju JH: Centrifugal gravity-induced BMP4 induces chondrogenic differentiation of adipose-derived stem cells via SOX9 upregulation. Stem Cell Res Ther. 7:1842016. View Article : Google Scholar : PubMed/NCBI

31 

Papadimitropoulos A, Scherberich A, Güven S, Theilgaard N, Crooijmans HJ, Santini F, Scheffler K, Zallone A and Martin I: A 3D in vitro bone organ model using human progenitor cells. Eur Cell Mater. 21:445–458. 2011. View Article : Google Scholar : PubMed/NCBI

32 

Leong DT, Abraham MC, Rath SN, Lim TC, Chew FT and Hutmacher DW: Investigating the effects of preinduction on human adipose-derived precursor cells in an athymic rat model. Differentiation. 74:519–529. 2006. View Article : Google Scholar : PubMed/NCBI

33 

Maglione M, Spano S, Ruaro ME, Salvador E, Zanconati F, Tromba G and Turco G: In vivo evaluation of chitosan-glycerol gel scaffolds seeded with stem cells for full-thickness mandibular bone regeneration. J Oral Sci. 59:225–232. 2017. View Article : Google Scholar : PubMed/NCBI

34 

Ye X, Liao C and Liu G: Age-related changes in the regenerative potential of adipose-derived stem cells isolated from the prominent fat pads in human lower. eyelids. 11:e01665902016.

35 

Kim S, Piao J, Son Y and Hong HS: Substance P enhances proliferation and paracrine potential of adipose-derived stem cells in vitro. Biochem Biophys Res Commun. 485:131–137. 2017. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Yang D, Li N and Zhang G: Spontaneous adipogenic differentiation potential of adipose‑derived stem cells decreased with increasing cell passages. Mol Med Rep 17: 6109-6115, 2018.
APA
Yang, D., Li, N., & Zhang, G. (2018). Spontaneous adipogenic differentiation potential of adipose‑derived stem cells decreased with increasing cell passages. Molecular Medicine Reports, 17, 6109-6115. https://doi.org/10.3892/mmr.2018.8619
MLA
Yang, D., Li, N., Zhang, G."Spontaneous adipogenic differentiation potential of adipose‑derived stem cells decreased with increasing cell passages". Molecular Medicine Reports 17.4 (2018): 6109-6115.
Chicago
Yang, D., Li, N., Zhang, G."Spontaneous adipogenic differentiation potential of adipose‑derived stem cells decreased with increasing cell passages". Molecular Medicine Reports 17, no. 4 (2018): 6109-6115. https://doi.org/10.3892/mmr.2018.8619
Copy and paste a formatted citation
x
Spandidos Publications style
Yang D, Li N and Zhang G: Spontaneous adipogenic differentiation potential of adipose‑derived stem cells decreased with increasing cell passages. Mol Med Rep 17: 6109-6115, 2018.
APA
Yang, D., Li, N., & Zhang, G. (2018). Spontaneous adipogenic differentiation potential of adipose‑derived stem cells decreased with increasing cell passages. Molecular Medicine Reports, 17, 6109-6115. https://doi.org/10.3892/mmr.2018.8619
MLA
Yang, D., Li, N., Zhang, G."Spontaneous adipogenic differentiation potential of adipose‑derived stem cells decreased with increasing cell passages". Molecular Medicine Reports 17.4 (2018): 6109-6115.
Chicago
Yang, D., Li, N., Zhang, G."Spontaneous adipogenic differentiation potential of adipose‑derived stem cells decreased with increasing cell passages". Molecular Medicine Reports 17, no. 4 (2018): 6109-6115. https://doi.org/10.3892/mmr.2018.8619
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team