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Article Open Access

MicroRNA-210 promotes angiogenesis in acute myocardial infarction

  • Authors:
    • Zhong‑Guo Fan
    • Xin‑Liang Qu
    • Peng Chu
    • Ya‑Li Gao
    • Xiao‑Fei Gao
    • Shao‑Liang Chen
    • Nai‑Liang Tian
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu 210006, P.R. China
    Copyright: © Fan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5658-5665
    |
    Published online on: February 20, 2018
       https://doi.org/10.3892/mmr.2018.8620
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Abstract

MicroRNA-210 (miRNA-210) has been reported to be associated with angiogenesis and may serve important roles in acute myocardial infarction (AMI), which remain unclear. The present study sought to evaluate the efficacy of miRNA‑210 in AMI and to examine the potential associated mechanisms. AMI models were established in Sprague‑Dawley rats. The expression of miRNA‑210 was upregulated via transfection with lentivirus‑mediated agonists and quantitative analysis was performed using the reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). Immunoblotting and RT‑qPCR were separately used to detect the expression levels of hepatocyte growth factor (HGF) in heart samples, while only the protein expression level of β‑myosin heavy chain (β‑MHC) was assessed. The expression of HGF in human umbilical vein endothelial cells under hypoxic conditions was silenced by transfecting with small interfering RNA, as demonstrated by the determination of associated protein expression levels. The microvessel density (MVD) of the infarcted myocardium was selected to be the angiogenesis efficacy endpoint, which was evaluated by platelet endothelial cell adhesion molecule immunostaining. Markedly increased expression of HGF was observed among the AMI rats receiving miRNA‑210 agonists, demonstrated via quantitative analyses using RT‑qPCR or western blotting. Promotion of angiogenesis was observed with the increased MVD. Improved cardiac function in the rats was subsequently noted, as they exhibited improved left ventricular fractional shortening and left ventricular ejection fraction percentages, which may result from improved cardiac contractility indicated by attenuating the increase in β‑MHC protein expression. Overexpression of miRNA‑210 appeared to be an advantageous therapeutic tool for treating AMI, primarily due to its promoting effects on angiogenesis in the infarcted myocardium by stimulating HGF expression and inducing improved left ventricular remodeling, leading to improved cardiac function.
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Copy and paste a formatted citation
Spandidos Publications style
Fan ZG, Qu XL, Chu P, Gao YL, Gao XF, Chen SL and Tian NL: MicroRNA-210 promotes angiogenesis in acute myocardial infarction. Mol Med Rep 17: 5658-5665, 2018.
APA
Fan, Z., Qu, X., Chu, P., Gao, Y., Gao, X., Chen, S., & Tian, N. (2018). MicroRNA-210 promotes angiogenesis in acute myocardial infarction. Molecular Medicine Reports, 17, 5658-5665. https://doi.org/10.3892/mmr.2018.8620
MLA
Fan, Z., Qu, X., Chu, P., Gao, Y., Gao, X., Chen, S., Tian, N."MicroRNA-210 promotes angiogenesis in acute myocardial infarction". Molecular Medicine Reports 17.4 (2018): 5658-5665.
Chicago
Fan, Z., Qu, X., Chu, P., Gao, Y., Gao, X., Chen, S., Tian, N."MicroRNA-210 promotes angiogenesis in acute myocardial infarction". Molecular Medicine Reports 17, no. 4 (2018): 5658-5665. https://doi.org/10.3892/mmr.2018.8620
Copy and paste a formatted citation
x
Spandidos Publications style
Fan ZG, Qu XL, Chu P, Gao YL, Gao XF, Chen SL and Tian NL: MicroRNA-210 promotes angiogenesis in acute myocardial infarction. Mol Med Rep 17: 5658-5665, 2018.
APA
Fan, Z., Qu, X., Chu, P., Gao, Y., Gao, X., Chen, S., & Tian, N. (2018). MicroRNA-210 promotes angiogenesis in acute myocardial infarction. Molecular Medicine Reports, 17, 5658-5665. https://doi.org/10.3892/mmr.2018.8620
MLA
Fan, Z., Qu, X., Chu, P., Gao, Y., Gao, X., Chen, S., Tian, N."MicroRNA-210 promotes angiogenesis in acute myocardial infarction". Molecular Medicine Reports 17.4 (2018): 5658-5665.
Chicago
Fan, Z., Qu, X., Chu, P., Gao, Y., Gao, X., Chen, S., Tian, N."MicroRNA-210 promotes angiogenesis in acute myocardial infarction". Molecular Medicine Reports 17, no. 4 (2018): 5658-5665. https://doi.org/10.3892/mmr.2018.8620
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