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miR‑205‑5p/PTK7 axis is involved in the proliferation, migration and invasion of colorectal cancer cells

  • Authors:
    • Shuo Chen
    • Yan Wang
    • Yinan Su
    • Lin Zhang
    • Mingqing Zhang
    • Xueqing Li
    • Juan Wang
    • Xipeng Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Colorectal Surgery, The People's Hospital of Tianjin, Tianjin 300121, P.R. China, Department of Pathology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250014, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 6253-6260
    |
    Published online on: February 28, 2018
       https://doi.org/10.3892/mmr.2018.8650
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Abstract

MicroRNAs (miRNAs) are small non‑coding RNAs, which are critical in a diverse range of biological processes, including development, differentiation, homeostasis, and in the formation of diseases by accelerating and/or inhibiting the translation of mRNAs. The present study aimed to examine the potential role of miRNA (miR)‑205‑5p in the developmental process of colorectal cancer (CRC) through protein‑tyrosine kinase 7 (PTK7). Initially, TargetScan was used to predict the miRNA target sites in the sequence of the PTK7 3'‑untranslated region. It was then found that the mRNA expression level of miR‑205‑5p was lower in CRC cells, determined using reverse transcription‑quantitative polymerase chain reaction analysis, and there was a negative correlation between miR‑205‑5p and PTK7 in CRC tissues. It was also found that miR‑205‑5p regulated the gene transcription of PTK7, determined using a luciferase reporter assay. The results of RT‑qPCR and western blot analyses in human colorectal cancer revealed that miR‑205‑5p suppressed the expression of PTK7. Finally, it was revealed that miR‑205‑5p restricted the proliferation ability of CRC cells through inhibiting PTK7, which was determined using colony forming and 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide assays. miR‑205‑5p accelerated cell apoptosis through inhibiting PTK7, demonstrated using Annexin V‑FITC/propidium iodide staining. The results of a Transwell assay indicated that miR‑205‑5p inhibited the migration and invasion abilities of CRC cells through inhibiting PTK7. Therefore, miR‑205‑5p is involved in the proliferation, migration and invasion of CRC through inhibiting PTK7.
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Copy and paste a formatted citation
Spandidos Publications style
Chen S, Wang Y, Su Y, Zhang L, Zhang M, Li X, Wang J and Zhang X: miR‑205‑5p/PTK7 axis is involved in the proliferation, migration and invasion of colorectal cancer cells. Mol Med Rep 17: 6253-6260, 2018.
APA
Chen, S., Wang, Y., Su, Y., Zhang, L., Zhang, M., Li, X. ... Zhang, X. (2018). miR‑205‑5p/PTK7 axis is involved in the proliferation, migration and invasion of colorectal cancer cells. Molecular Medicine Reports, 17, 6253-6260. https://doi.org/10.3892/mmr.2018.8650
MLA
Chen, S., Wang, Y., Su, Y., Zhang, L., Zhang, M., Li, X., Wang, J., Zhang, X."miR‑205‑5p/PTK7 axis is involved in the proliferation, migration and invasion of colorectal cancer cells". Molecular Medicine Reports 17.5 (2018): 6253-6260.
Chicago
Chen, S., Wang, Y., Su, Y., Zhang, L., Zhang, M., Li, X., Wang, J., Zhang, X."miR‑205‑5p/PTK7 axis is involved in the proliferation, migration and invasion of colorectal cancer cells". Molecular Medicine Reports 17, no. 5 (2018): 6253-6260. https://doi.org/10.3892/mmr.2018.8650
Copy and paste a formatted citation
x
Spandidos Publications style
Chen S, Wang Y, Su Y, Zhang L, Zhang M, Li X, Wang J and Zhang X: miR‑205‑5p/PTK7 axis is involved in the proliferation, migration and invasion of colorectal cancer cells. Mol Med Rep 17: 6253-6260, 2018.
APA
Chen, S., Wang, Y., Su, Y., Zhang, L., Zhang, M., Li, X. ... Zhang, X. (2018). miR‑205‑5p/PTK7 axis is involved in the proliferation, migration and invasion of colorectal cancer cells. Molecular Medicine Reports, 17, 6253-6260. https://doi.org/10.3892/mmr.2018.8650
MLA
Chen, S., Wang, Y., Su, Y., Zhang, L., Zhang, M., Li, X., Wang, J., Zhang, X."miR‑205‑5p/PTK7 axis is involved in the proliferation, migration and invasion of colorectal cancer cells". Molecular Medicine Reports 17.5 (2018): 6253-6260.
Chicago
Chen, S., Wang, Y., Su, Y., Zhang, L., Zhang, M., Li, X., Wang, J., Zhang, X."miR‑205‑5p/PTK7 axis is involved in the proliferation, migration and invasion of colorectal cancer cells". Molecular Medicine Reports 17, no. 5 (2018): 6253-6260. https://doi.org/10.3892/mmr.2018.8650
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