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High expression of active ATF6 aggravates endoplasmic reticulum stress‑induced vascular endothelial cell apoptosis through the mitochondrial apoptotic pathway

  • Authors:
    • Jingyong Huang
    • Li Wan
    • Heping Lu
    • Xiaoqiang Li
  • View Affiliations / Copyright

    Affiliations: Department of Vascular Surgery, The Second Affiliated Hospital of Soochow University, Soochow, Jiangsu 215000, P.R. China, Department of Pathology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China, Department of Vascular Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China
    Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 6483-6489
    |
    Published online on: March 1, 2018
       https://doi.org/10.3892/mmr.2018.8658
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Abstract

Activating transcription factor 6 (ATF6), one of three sensor proteins in the endoplasmic reticulum (ER), is an important regulatory factor in the ER stress‑induced apoptosis pathway. Although recent studies have made some progress in elucidating the regulation mechanism of ATF6, the specific regulatory mechanism of ER stress‑induced vascular endothelial cell (VEC) apoptosis is still unclear. The present study was designed to investigate the role of ATF6 in VECs under thapsigargin (TG)‑induced ER stress. ATF6 (1‑366aa; ATF6 high‑expressed plasmid) and ATF6 (151‑366aa; plasmid without transcriptional activity) were transfected into VECs to yield an ATF6 high‑expression model and a positive control model, respectively. High expression of ATF6 decreased viability and aggravated ER stress‑induced apoptosis in VECs. Increased expression of apoptosis‑related genes, including those encoding caspase‑3, caspase‑9, C/EBP homologous protein (CHOP), cytochrome c and B‑cell lymphoma‑associated protein X (Bax)/B‑cell lymphoma (Bcl‑)2, was detected by polymerase chain reaction and western blotting in the ATF6 (1‑366aa) + TG group. No significant effect of TG treatment and high ATF6 expression was indicated on the expression of death receptor‑related genes, including those encoding caspase‑8 and Fas. The results demonstrated that high expression of activated ATF6 aggravates ER stress‑induced VEC apoptosis through the mitochondrial apoptotic pathway. Furthermore, in response to ER stress, ATF6 upregulates the expression of caspase‑3, caspase‑9, CHOP, cytochrome c and Bax/Bcl‑2.
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Copy and paste a formatted citation
Spandidos Publications style
Huang J, Wan L, Lu H and Li X: High expression of active ATF6 aggravates endoplasmic reticulum stress‑induced vascular endothelial cell apoptosis through the mitochondrial apoptotic pathway. Mol Med Rep 17: 6483-6489, 2018.
APA
Huang, J., Wan, L., Lu, H., & Li, X. (2018). High expression of active ATF6 aggravates endoplasmic reticulum stress‑induced vascular endothelial cell apoptosis through the mitochondrial apoptotic pathway. Molecular Medicine Reports, 17, 6483-6489. https://doi.org/10.3892/mmr.2018.8658
MLA
Huang, J., Wan, L., Lu, H., Li, X."High expression of active ATF6 aggravates endoplasmic reticulum stress‑induced vascular endothelial cell apoptosis through the mitochondrial apoptotic pathway". Molecular Medicine Reports 17.5 (2018): 6483-6489.
Chicago
Huang, J., Wan, L., Lu, H., Li, X."High expression of active ATF6 aggravates endoplasmic reticulum stress‑induced vascular endothelial cell apoptosis through the mitochondrial apoptotic pathway". Molecular Medicine Reports 17, no. 5 (2018): 6483-6489. https://doi.org/10.3892/mmr.2018.8658
Copy and paste a formatted citation
x
Spandidos Publications style
Huang J, Wan L, Lu H and Li X: High expression of active ATF6 aggravates endoplasmic reticulum stress‑induced vascular endothelial cell apoptosis through the mitochondrial apoptotic pathway. Mol Med Rep 17: 6483-6489, 2018.
APA
Huang, J., Wan, L., Lu, H., & Li, X. (2018). High expression of active ATF6 aggravates endoplasmic reticulum stress‑induced vascular endothelial cell apoptosis through the mitochondrial apoptotic pathway. Molecular Medicine Reports, 17, 6483-6489. https://doi.org/10.3892/mmr.2018.8658
MLA
Huang, J., Wan, L., Lu, H., Li, X."High expression of active ATF6 aggravates endoplasmic reticulum stress‑induced vascular endothelial cell apoptosis through the mitochondrial apoptotic pathway". Molecular Medicine Reports 17.5 (2018): 6483-6489.
Chicago
Huang, J., Wan, L., Lu, H., Li, X."High expression of active ATF6 aggravates endoplasmic reticulum stress‑induced vascular endothelial cell apoptosis through the mitochondrial apoptotic pathway". Molecular Medicine Reports 17, no. 5 (2018): 6483-6489. https://doi.org/10.3892/mmr.2018.8658
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