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Article

Tumor necrosis factor-α downregulates the REIC/Dkk-3 tumor suppressor gene in normal human skin keratinocytes

  • Authors:
    • Ken Kataoka
    • Natsumi Maehara
    • Yuki Ayabe
    • Hitoshi Murata
    • Nam‑Ho Huh
    • Masakiyo Sakaguchi
  • View Affiliations / Copyright

    Affiliations: Department of Life Science, Faculty of Science, Okayama University of Science, Okayama 700‑0005, Japan, Department of Cell Biology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700‑8558, Japan
  • Pages: 6661-6666
    |
    Published online on: March 5, 2018
       https://doi.org/10.3892/mmr.2018.8676
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Abstract

Our previous studies revealed that REIC/Dkk-3 was expressed various tissues, including skin keratinocytes. The aim of the present study was to identify the factors that regulate the expression of the dickkopf Wnt signaling pathway inhibitor 3 (REIC/Dkk‑3) tumor suppressor gene in normal human skin keratinocytes (NHKs). Several growth factors and cytokines that have previously been reported to be involved in the growth and differentiation of keratinocytes were screened as potential regulators. Western blot analysis was performed using protein from NHKs cultured with/without various factors including the epidermal growth factor, tumor necrosis factor‑α, transforming growth factor‑β, interleukin (IL)‑1F9, IL‑6, IL‑8 and Ca2+. The results indicated that only TNF‑α downregulated REIC/Dkk‑3 expression in NHKs. Subsequently, TNF‑α was confirmed to reduce the expression levels of REIC/Dkk‑3 in mouse skin tissue and hair culture models. TNF‑α‑mediated downregulation of REIC/Dkk‑3 expression in NHKs was abrogated by the addition of a TNF‑α‑specific antibody. In conclusion, the results indicate that TNF‑α downregulates REIC/Dkk‑3 expression in normal skin keratinocytes.
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Copy and paste a formatted citation
Spandidos Publications style
Kataoka K, Maehara N, Ayabe Y, Murata H, Huh NH and Sakaguchi M: Tumor necrosis factor-α downregulates the REIC/Dkk-3 tumor suppressor gene in normal human skin keratinocytes. Mol Med Rep 17: 6661-6666, 2018.
APA
Kataoka, K., Maehara, N., Ayabe, Y., Murata, H., Huh, N., & Sakaguchi, M. (2018). Tumor necrosis factor-α downregulates the REIC/Dkk-3 tumor suppressor gene in normal human skin keratinocytes. Molecular Medicine Reports, 17, 6661-6666. https://doi.org/10.3892/mmr.2018.8676
MLA
Kataoka, K., Maehara, N., Ayabe, Y., Murata, H., Huh, N., Sakaguchi, M."Tumor necrosis factor-α downregulates the REIC/Dkk-3 tumor suppressor gene in normal human skin keratinocytes". Molecular Medicine Reports 17.5 (2018): 6661-6666.
Chicago
Kataoka, K., Maehara, N., Ayabe, Y., Murata, H., Huh, N., Sakaguchi, M."Tumor necrosis factor-α downregulates the REIC/Dkk-3 tumor suppressor gene in normal human skin keratinocytes". Molecular Medicine Reports 17, no. 5 (2018): 6661-6666. https://doi.org/10.3892/mmr.2018.8676
Copy and paste a formatted citation
x
Spandidos Publications style
Kataoka K, Maehara N, Ayabe Y, Murata H, Huh NH and Sakaguchi M: Tumor necrosis factor-α downregulates the REIC/Dkk-3 tumor suppressor gene in normal human skin keratinocytes. Mol Med Rep 17: 6661-6666, 2018.
APA
Kataoka, K., Maehara, N., Ayabe, Y., Murata, H., Huh, N., & Sakaguchi, M. (2018). Tumor necrosis factor-α downregulates the REIC/Dkk-3 tumor suppressor gene in normal human skin keratinocytes. Molecular Medicine Reports, 17, 6661-6666. https://doi.org/10.3892/mmr.2018.8676
MLA
Kataoka, K., Maehara, N., Ayabe, Y., Murata, H., Huh, N., Sakaguchi, M."Tumor necrosis factor-α downregulates the REIC/Dkk-3 tumor suppressor gene in normal human skin keratinocytes". Molecular Medicine Reports 17.5 (2018): 6661-6666.
Chicago
Kataoka, K., Maehara, N., Ayabe, Y., Murata, H., Huh, N., Sakaguchi, M."Tumor necrosis factor-α downregulates the REIC/Dkk-3 tumor suppressor gene in normal human skin keratinocytes". Molecular Medicine Reports 17, no. 5 (2018): 6661-6666. https://doi.org/10.3892/mmr.2018.8676
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