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Downregulation of the long non-coding RNA ZFAS1 is associated with cell proliferation, migration and invasion in breast cancer

  • Authors:
    • Shulin Fan
    • Chunni Fan
    • Ning Liu
    • Keke Huang
    • Xuedong Fang
    • Keren Wang
  • View Affiliations / Copyright

    Affiliations: Department of Breast Surgery, China‑Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China, State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University, Changchun, Jilin 130012, P.R. China, Department of Gastrointestinal Surgery, China‑Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China
    Copyright: © Fan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 6405-6412
    |
    Published online on: March 9, 2018
       https://doi.org/10.3892/mmr.2018.8707
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Abstract

Long non-coding RNAs (lncRNAs) are non‑coding RNAs that are >200 nucleotides in length. Recent studies have identified a number of lncRNAs with critical roles in various biological processes including tumorigenesis. Zinc finger antisense 1 (ZFAS1) is a lncRNA that has recently been reported to be involved in the progression of several human cancers. However, the biological function of ZFAS1 in breast cancer remains to be elucidated. In order to determine the effect of ZFAS1 in breast cancer cells, reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) was performed to measure ZFAS1 expression in cells from breast cancer cell lines. In addition, gain‑of‑function experiments were performed in vitro to investigate the biological role of ZFAS1. The results revealed that ZFAS1 expression was significantly downregulated in breast cancer cell lines when compared with the levels in controls. In vitro experiments also demonstrated that ZFAS1 overexpression significantly suppressed cell proliferation by causing cell cycle arrest and inducing apoptosis in breast cancer cells. Further functional assays indicated that ZFAS1 overexpression inhibited cell migration and invasion by regulating epithelial‑mesenchymal transition. These findings indicated that the lncRNA ZFAS1 may be a tumor suppressor in breast cancer, and thus, may serve as a potential therapeutic target for patients with breast cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Fan S, Fan C, Liu N, Huang K, Fang X and Wang K: Downregulation of the long non-coding RNA ZFAS1 is associated with cell proliferation, migration and invasion in breast cancer. Mol Med Rep 17: 6405-6412, 2018.
APA
Fan, S., Fan, C., Liu, N., Huang, K., Fang, X., & Wang, K. (2018). Downregulation of the long non-coding RNA ZFAS1 is associated with cell proliferation, migration and invasion in breast cancer. Molecular Medicine Reports, 17, 6405-6412. https://doi.org/10.3892/mmr.2018.8707
MLA
Fan, S., Fan, C., Liu, N., Huang, K., Fang, X., Wang, K."Downregulation of the long non-coding RNA ZFAS1 is associated with cell proliferation, migration and invasion in breast cancer". Molecular Medicine Reports 17.5 (2018): 6405-6412.
Chicago
Fan, S., Fan, C., Liu, N., Huang, K., Fang, X., Wang, K."Downregulation of the long non-coding RNA ZFAS1 is associated with cell proliferation, migration and invasion in breast cancer". Molecular Medicine Reports 17, no. 5 (2018): 6405-6412. https://doi.org/10.3892/mmr.2018.8707
Copy and paste a formatted citation
x
Spandidos Publications style
Fan S, Fan C, Liu N, Huang K, Fang X and Wang K: Downregulation of the long non-coding RNA ZFAS1 is associated with cell proliferation, migration and invasion in breast cancer. Mol Med Rep 17: 6405-6412, 2018.
APA
Fan, S., Fan, C., Liu, N., Huang, K., Fang, X., & Wang, K. (2018). Downregulation of the long non-coding RNA ZFAS1 is associated with cell proliferation, migration and invasion in breast cancer. Molecular Medicine Reports, 17, 6405-6412. https://doi.org/10.3892/mmr.2018.8707
MLA
Fan, S., Fan, C., Liu, N., Huang, K., Fang, X., Wang, K."Downregulation of the long non-coding RNA ZFAS1 is associated with cell proliferation, migration and invasion in breast cancer". Molecular Medicine Reports 17.5 (2018): 6405-6412.
Chicago
Fan, S., Fan, C., Liu, N., Huang, K., Fang, X., Wang, K."Downregulation of the long non-coding RNA ZFAS1 is associated with cell proliferation, migration and invasion in breast cancer". Molecular Medicine Reports 17, no. 5 (2018): 6405-6412. https://doi.org/10.3892/mmr.2018.8707
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