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Detection of epitopes in systemic lupus erythematosus using peptide microarray

  • Authors:
    • Xin Li
    • Haixia Li
    • Qiongdan Hu
    • Jinfei Lin
    • Qiong Zhang
    • Yao Li
    • Juan Li
    • Tao Chen
    • Yurong Qiu
  • View Affiliations / Copyright

    Affiliations: Clinical Laboratory of Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, Department of Nephrology, The Traditional Chinese Medicine Hospital, Affiliated to Southwest Medical University, Luzhou, Sichuan 646000, P.R. China, South China Institute of Microbial Ecology and Health, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, Department of Rheumatology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 6533-6541
    |
    Published online on: March 9, 2018
       https://doi.org/10.3892/mmr.2018.8710
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Abstract

Systemic lupus erythematosus (SLE) is a common autoimmune disease, which features the secretion of antibodies directed against autoantigens in vivo. In the present study, a peptide microarray was developed to detect the epitopes recognized by autoantibodies in patients with SLE for an effective method of diagnosis. SLE‑associated epitopes in 14 autoantigens were predicted using the antigenic epitope prediction software DNA star. Peptides were synthesized based on the predicted antigenic epitopes and immobilized on a slide surface and developed into a peptide microarray. Using this peptide microarray the autoantibodies in 120 patients with SLE and 110 healthy subjects were detected. A total of 73 potential antigenic epitopes in 14 autoantigens were predicted and screened. The peptide microarray based on the 73 epitopes was used to detect the autoantibodies in patients with SLE. A total of 14 epitopes with potential diagnostic values were screened out. The sensitivity and specificity of the 14 epitopes for the diagnosis of SLE were 71.6 and 85.8%, respectively. An optimal set of epitopes for SLE diagnosis was obtained. As individual patients had a specific autoantibody spectrum it was possible to detect autoantibodies in SLE and perform the diagnosis of SLE using the peptide microarray.
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Copy and paste a formatted citation
Spandidos Publications style
Li X, Li H, Hu Q, Lin J, Zhang Q, Li Y, Li J, Chen T and Qiu Y: Detection of epitopes in systemic lupus erythematosus using peptide microarray. Mol Med Rep 17: 6533-6541, 2018.
APA
Li, X., Li, H., Hu, Q., Lin, J., Zhang, Q., Li, Y. ... Qiu, Y. (2018). Detection of epitopes in systemic lupus erythematosus using peptide microarray. Molecular Medicine Reports, 17, 6533-6541. https://doi.org/10.3892/mmr.2018.8710
MLA
Li, X., Li, H., Hu, Q., Lin, J., Zhang, Q., Li, Y., Li, J., Chen, T., Qiu, Y."Detection of epitopes in systemic lupus erythematosus using peptide microarray". Molecular Medicine Reports 17.5 (2018): 6533-6541.
Chicago
Li, X., Li, H., Hu, Q., Lin, J., Zhang, Q., Li, Y., Li, J., Chen, T., Qiu, Y."Detection of epitopes in systemic lupus erythematosus using peptide microarray". Molecular Medicine Reports 17, no. 5 (2018): 6533-6541. https://doi.org/10.3892/mmr.2018.8710
Copy and paste a formatted citation
x
Spandidos Publications style
Li X, Li H, Hu Q, Lin J, Zhang Q, Li Y, Li J, Chen T and Qiu Y: Detection of epitopes in systemic lupus erythematosus using peptide microarray. Mol Med Rep 17: 6533-6541, 2018.
APA
Li, X., Li, H., Hu, Q., Lin, J., Zhang, Q., Li, Y. ... Qiu, Y. (2018). Detection of epitopes in systemic lupus erythematosus using peptide microarray. Molecular Medicine Reports, 17, 6533-6541. https://doi.org/10.3892/mmr.2018.8710
MLA
Li, X., Li, H., Hu, Q., Lin, J., Zhang, Q., Li, Y., Li, J., Chen, T., Qiu, Y."Detection of epitopes in systemic lupus erythematosus using peptide microarray". Molecular Medicine Reports 17.5 (2018): 6533-6541.
Chicago
Li, X., Li, H., Hu, Q., Lin, J., Zhang, Q., Li, Y., Li, J., Chen, T., Qiu, Y."Detection of epitopes in systemic lupus erythematosus using peptide microarray". Molecular Medicine Reports 17, no. 5 (2018): 6533-6541. https://doi.org/10.3892/mmr.2018.8710
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