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Article Open Access

Identification of the key genes associated with neuropathic pain

  • Authors:
    • Huan Liu
    • Tianjiao Xia
    • Fangxia Xu
    • Zhengliang Ma
    • Xiaoping Gu
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, Affiliated Nanjing Drum Tower Hospital of Nanjing University School of Medicine, Nanjing, Jiangsu 210008, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 6371-6378
    |
    Published online on: March 9, 2018
       https://doi.org/10.3892/mmr.2018.8718
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Abstract

Neuropathic pain is a chronic pain state associated with multiple etiologies that results in considerable social and economic burden. The identification of key genes associated with neuropathic pain is important for the development of novel therapies. Therefore, the present study downloaded the gene expression profile GSE15041 from the Gene Expression Omnibus database. The unverified gene chip was removed and the microarray data was normalized following quality control. The limma package in R was used to screen the differentially expressed genes (DEGs), followed by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. Furthermore, a protein‑protein interaction (PPI) network based on the identified DEGs was constructed to select hub proteins, and reverse transcription‑quantitative polymerase chain reaction was performed to detect the expression of these proteins in a mouse model of neuropathic pain. In total, 86 common DEGs were identified. DEGs were significantly enriched in ̔extracellular space̓ and KEGG pathway enrichment analysis demonstrated that the DEGs were significantly enriched in inflammatory diseases and the mitogen‑activated protein kinase signaling pathway. The PPI network consisted of 27 nodes (proteins) and 47 PPI edges (interactions). Interleukin (IL)‑6, transcription factor AP‑1 (c‑Jun) and urikinase‑type plasminogen activator (Plau) were identified as hub proteins and key genes in neuropathic pain. The mRNA expression of these hub proteins was significantly increased in the neuropathic pain model, compared with the sham group. IL‑6, c‑Jun, and Plau may be involved in development of neuropathic pain and further research investigating the exact role of these key genes is required.
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Copy and paste a formatted citation
Spandidos Publications style
Liu H, Xia T, Xu F, Ma Z and Gu X: Identification of the key genes associated with neuropathic pain. Mol Med Rep 17: 6371-6378, 2018.
APA
Liu, H., Xia, T., Xu, F., Ma, Z., & Gu, X. (2018). Identification of the key genes associated with neuropathic pain. Molecular Medicine Reports, 17, 6371-6378. https://doi.org/10.3892/mmr.2018.8718
MLA
Liu, H., Xia, T., Xu, F., Ma, Z., Gu, X."Identification of the key genes associated with neuropathic pain". Molecular Medicine Reports 17.5 (2018): 6371-6378.
Chicago
Liu, H., Xia, T., Xu, F., Ma, Z., Gu, X."Identification of the key genes associated with neuropathic pain". Molecular Medicine Reports 17, no. 5 (2018): 6371-6378. https://doi.org/10.3892/mmr.2018.8718
Copy and paste a formatted citation
x
Spandidos Publications style
Liu H, Xia T, Xu F, Ma Z and Gu X: Identification of the key genes associated with neuropathic pain. Mol Med Rep 17: 6371-6378, 2018.
APA
Liu, H., Xia, T., Xu, F., Ma, Z., & Gu, X. (2018). Identification of the key genes associated with neuropathic pain. Molecular Medicine Reports, 17, 6371-6378. https://doi.org/10.3892/mmr.2018.8718
MLA
Liu, H., Xia, T., Xu, F., Ma, Z., Gu, X."Identification of the key genes associated with neuropathic pain". Molecular Medicine Reports 17.5 (2018): 6371-6378.
Chicago
Liu, H., Xia, T., Xu, F., Ma, Z., Gu, X."Identification of the key genes associated with neuropathic pain". Molecular Medicine Reports 17, no. 5 (2018): 6371-6378. https://doi.org/10.3892/mmr.2018.8718
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