Benzoxazole derivatives suppress lipopolysaccharide-induced mast cell activation

Cho,Kyung‑Ah; Park,Minhwa; Kim,Yu‑Hee; Choo,Hea‑Young  Park; Lee,Kyung  Ho

doi:10.3892/mmr.2018.8719

Benzoxazole derivatives suppress lipopolysaccharide-induced mast cell activation

Authors:
- Kyung‑Ah Cho
- Minhwa Park
- Yu‑Hee Kim
- Hea‑Young Park Choo
- Kyung Ho Lee
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Affiliations: Department of Microbiology, School of Medicine, Ewha Womans University, Seoul 07985, Republic of Korea, College of Pharmacy and Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul 03760, Republic of Korea, Department of Dermatology, Bucheon St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Bucheon‑si 14647, Republic of Korea
Published online on: March 9, 2018 https://doi.org/10.3892/mmr.2018.8719
Pages: 6723-6730

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Abstract

Mast cells are central regulators of allergic inflammation that function by releasing various proallergic inflammatory mediators, including histamine, eicosanoids and proinflammatory cytokines. Occasionally, bacterial infections may initiate or worsen allergic inflammation. A number of studies have indicated that activation of lipoxygenase in mast cells positive regulates allergic inflammatory responses by generating leukotrienes and proinflammatory cytokines. In the present study, the effects of benzoxazole derivatives on the lipopolysaccharide (LPS)‑induced expression of proinflammatory cytokines, production of histamine and surface expression of co‑stimulatory molecules on bone marrow-derived mast cells (BMMCs) were studied. The benzoxazole derivatives significantly reduced the expression of interleukin (IL)‑1β, IL‑6, IL‑13, tumor necrosis factor‑α, perilipin (PLIN) 2, and PLIN3 in BMMCs treated with LPS. Furthermore, histamine production was suppressed in BMMCs treated with LPS, or treated with phorbol-12-myristate-13-acetate/ionomycin. Benzoxazole derivatives marginally affected the surface expression of cluster of differentiation (CD)80 and CD86 on BMMCs in the presence of LPS, although LPS alone did not increase the expression of those proteins. Therefore, benzoxazole derivatives inhibited the secretion of proinflammatory cytokines in mast cells and may be potential candidate anti‑allergic agents to suppress mast cell activation.

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