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Atorvastatin prevents glomerular extracellular matrix formation by interfering with the PKC signaling pathway

  • Authors:
    • Yan‑Hua Xiao
    • Xiao‑Yun He
    • Qing Han
    • Fan Yang
    • Su‑Xian Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Endocrinology, Affiliated Hospital of Guilin Medical University, Guilin, Guangxi 541001, P.R. China
    Copyright: © Xiao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 6441-6448
    |
    Published online on: March 9, 2018
       https://doi.org/10.3892/mmr.2018.8724
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Abstract

Platelet-activating factor (PAF) promotes glomerular extracellular matrix (ECM) deposition, primarily through activation of the protein kinase C (PKC) pathway. The present study was designed to investigate whether atorvastatin, which mediates a protective effect against glomerular ECM deposition and diabetic neuropathy, may interfere with the PKC‑transforming growth factor‑β1 (TGF‑β1) pathway in a model of human mesangial cells (HMCs) exposed to a high glucose (HG) and lysophosphatidylcholine (LPC) environment. HMCs were divided into three treatment groups: Control, high glucose and lysophosphatidylcholine (HG+LPC), and HG+LPC+atorvastatin. Cells were cultured for 24 h. The levels of the ECM‑associated molecules collagen IV (Col IV) and fibronectin (Fn) in the supernatant were detected using an ELISA kit. PKC‑β1, TGF‑β1 and PAF‑receptor gene expression was detected by reverse transcription‑quantitative polymerase chain reaction. PKC‑β1 and TGF‑β1 protein expression was detected by western blotting, and the subcellular localization of PKC‑β1 was assessed using immunofluorescence. The results indicated that atorvastatin may reduce the secretion of ECM components (Fn and Col IV) in HMCs in a HG and LPC environment, by inhibiting the increase in PAF secretion and the activation of the PKC‑TGF‑β1 signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Xiao YH, He XY, Han Q, Yang F and Zhou SX: Atorvastatin prevents glomerular extracellular matrix formation by interfering with the PKC signaling pathway. Mol Med Rep 17: 6441-6448, 2018.
APA
Xiao, Y., He, X., Han, Q., Yang, F., & Zhou, S. (2018). Atorvastatin prevents glomerular extracellular matrix formation by interfering with the PKC signaling pathway. Molecular Medicine Reports, 17, 6441-6448. https://doi.org/10.3892/mmr.2018.8724
MLA
Xiao, Y., He, X., Han, Q., Yang, F., Zhou, S."Atorvastatin prevents glomerular extracellular matrix formation by interfering with the PKC signaling pathway". Molecular Medicine Reports 17.5 (2018): 6441-6448.
Chicago
Xiao, Y., He, X., Han, Q., Yang, F., Zhou, S."Atorvastatin prevents glomerular extracellular matrix formation by interfering with the PKC signaling pathway". Molecular Medicine Reports 17, no. 5 (2018): 6441-6448. https://doi.org/10.3892/mmr.2018.8724
Copy and paste a formatted citation
x
Spandidos Publications style
Xiao YH, He XY, Han Q, Yang F and Zhou SX: Atorvastatin prevents glomerular extracellular matrix formation by interfering with the PKC signaling pathway. Mol Med Rep 17: 6441-6448, 2018.
APA
Xiao, Y., He, X., Han, Q., Yang, F., & Zhou, S. (2018). Atorvastatin prevents glomerular extracellular matrix formation by interfering with the PKC signaling pathway. Molecular Medicine Reports, 17, 6441-6448. https://doi.org/10.3892/mmr.2018.8724
MLA
Xiao, Y., He, X., Han, Q., Yang, F., Zhou, S."Atorvastatin prevents glomerular extracellular matrix formation by interfering with the PKC signaling pathway". Molecular Medicine Reports 17.5 (2018): 6441-6448.
Chicago
Xiao, Y., He, X., Han, Q., Yang, F., Zhou, S."Atorvastatin prevents glomerular extracellular matrix formation by interfering with the PKC signaling pathway". Molecular Medicine Reports 17, no. 5 (2018): 6441-6448. https://doi.org/10.3892/mmr.2018.8724
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