Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
May-2018 Volume 17 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
May-2018 Volume 17 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Pyrroloquinoline quinone induces chondrosarcoma cell apoptosis by increasing intracellular reactive oxygen species

  • Authors:
    • Linchun Wen
    • Xiyan Lu
    • Rui Wang
    • Xiaowei Jin
    • Liqiang Hu
    • Chuanwen You
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, Suqian People's Hospital of Nanjing Drum Tower Hospital Group, Suqian, Jiangsu 223800, P.R. China, Department of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310009, P.R. China
    Copyright: © Wen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 7184-7190
    |
    Published online on: March 14, 2018
       https://doi.org/10.3892/mmr.2018.8745
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Pyrroloquinoline quinone (PQQ) has been reported to contribute to cancer cell apoptosis and death; however, little is known of its underlying mechanisms. The present study was designed to investigate the role of PQQ in chondrosarcoma cell apoptosis and the underlying mechanism. A cell cytotoxicity assay was used to detect cell death; flow cytometry analysis was also performed to determine cell apoptosis and intracellular reactive oxygen species (ROS). Biochemical methods were employed to detect the activity and the expression of superoxide dismutase (SOD)1, SOD2 and glutathione. The present study also examined the effect on tumorigenesis in vivo. The results demonstrated that the apoptosis of SW1353 cells induced by PQQ increased in a concentration‑ and time‑dependent manner, which may be attributable to the accumulation of intracellular ROS. In the in vivo experiments, PQQ inhibited proliferation and promoted apoptosis, increased ROS levels and caused DNA damage in transplanted cells. Taken together, the findings of the present study confirmed that PQQ induced apoptosis in human chondrosarcoma SW1353 cells and transplanted cells, by increasing intracellular ROS and reducing the ability of scavenging oxygen free radicals.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Bauer HC, Brosjö O, Kreicbergs A and Lindholm J: Low risk of recurrence of enchondroma and low-grade chondrosarcoma in extremities. 80 patients followed for 2–25 years. Acta Orthop Scand. 66:283–288. 1995. View Article : Google Scholar : PubMed/NCBI

2 

Eriksson AI, Schiller A and Mankin HJ: The management of chondrosarcoma of bone. Clin Orthop Relat Res. 44–66. 1980.PubMed/NCBI

3 

Bovée JV, Cleton-Jansen AM, Taminiau AH and Hogendoorn PC: Emerging pathways in the development of chondrosarcoma of bone and implications for targeted treatment. Lancet Oncol. 6:599–607. 2005. View Article : Google Scholar : PubMed/NCBI

4 

Bao X, Ren T, Huang Y, Wang S, Zhang F, Liu K, Zheng B and Guo W: Induction of the mesenchymal to epithelial transition by demethylation-activated microRNA-125b is involved in the anti-migration/invasion effects of arsenic trioxide on human chondrosarcoma. J Exp Clin Cancer Res. 35:1292016. View Article : Google Scholar : PubMed/NCBI

5 

McIntire WS: Newly discovered redox cofactors: Possible nutritional, medical, and pharmacological relevance to higher animals. Annu Rev Nutr. 18:145–177. 1998. View Article : Google Scholar : PubMed/NCBI

6 

Misra HS, Khairnar NP, Barik A, Indira Priyadarsini K, Mohan H and Apte SK: Pyrroloquinoline-quinone: A reactive oxygen species scavenger in bacteria. FEBS Lett. 578:26–30. 2004. View Article : Google Scholar : PubMed/NCBI

7 

He K, Nukada H, Urakami T and Murphy MP: Antioxidant and pro-oxidant properties of pyrroloquinoline quinone (PQQ): Implications for its function in biological systems. Biochem Pharmacol. 65:67–74. 2003. View Article : Google Scholar : PubMed/NCBI

8 

Smidt CR, Steinberg FM and Rucker RB: Physiologic importance of pyrroloquinoline quinone. Proc Soc Exp Biol Med. 197:pp. 19–26. 1991; View Article : Google Scholar : PubMed/NCBI

9 

Bishop A, Gallop PM and Karnovsky ML: Pyrroloquinoline quinone: A novel vitamin? Nutr Rev. 56:287–293. 1998. View Article : Google Scholar : PubMed/NCBI

10 

Shankar BS, Pandey R, Amin P, Misra HS and Sainis KB: Role of glutathione in augmenting the anticancer activity of pyrroloquinoline quinone (PQQ). Redox Rep. 15:146–154. 2010. View Article : Google Scholar : PubMed/NCBI

11 

Min Z, Wang L, Jin J, Wang X, Zhu B, Chen H and Cheng Y: Pyrroloquinoline quinone induces cancer cell apoptosis via mitochondrial-dependent pathway and down-regulating cellular Bcl-2 protein expression. J Cancer. 5:609–624. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Pizzimenti S, Toaldo C, Pettazzoni P, Dianzani MU and Barrera G: Thetwo-faced effects of reactive oxygen species and the lipid peroxidation product4-hydroxynonenal in the hallmarks of cancer. Cancers (Basel). 2:338–363. 2010. View Article : Google Scholar : PubMed/NCBI

13 

Tripathi DN, Chowdhury R, Trudel LJ, Tee AR, Slack RS, Walker CL and Wogan GN: Reactive nitrogen species regulate autophagy through ATM-AMPK-TSC2 mediated suppression of mTORC1. Proc Natl Acad Sci USA. 110:pp. E2950–E2957. 2013; View Article : Google Scholar : PubMed/NCBI

14 

Finkel T and Holbrook NJ: Oxidants, oxidative stress and the biology of ageing. Nature. 408:239–247. 2000. View Article : Google Scholar : PubMed/NCBI

15 

Martindale JL and Holbrook NJ: Cellular response to oxidative stress: Signalling for suicide and survival. J Cell Physiol. 192:1–15. 2002. View Article : Google Scholar : PubMed/NCBI

16 

Fang J, Nakamura H and Iyer AK: Regulation of replicative potential of cells. J Drug Target. 15:475–486. 2007. View Article : Google Scholar : PubMed/NCBI

17 

Baliga R, Zhang Z, Baliga M, Ueda N and Shah SV: In vitro and in vivo evidence suggesting a role for iron in cisplatin-induced nephrotoxicity. Kidney Int. 53:394–401. 1998. View Article : Google Scholar : PubMed/NCBI

18 

Xie X, Zhao Y, Ma CY, Xu XM, Zhang YQ, Wang CG, Jin J, Shen X, Gao JL, Li N, et al: Dimethyl fumarate induces necroptosis in colon cancer cells through GSH depletion/ROS increase/MAPKs activation pathway. Br J Pharmacol. 172:3929–3943. 2015. View Article : Google Scholar : PubMed/NCBI

19 

Wang X, Bao Y, Dong Z, Chen Q, Guo H, Ziang C and Shao J: WP1130 attenuates cisplatin resistance by decreasing P53 expression in non-small cell lung carcinomas. Oncotarget. 8:49033–49043. 2017.PubMed/NCBI

20 

Salisbury SA, Forrest HS, Cruse WB and Kennard O: A novel coenzyme from bacterial primary alcohol dehydrogenases. Nature. 280:843–844. 1979. View Article : Google Scholar : PubMed/NCBI

21 

Killgore J, Smidt C, Duich L, Romero-Chapman N, Tinker D, Reiser K, Melko M, Hyde D and Rucker RB: Nutritional importance of pyrroloquinoline quinone. Science. 245:850–852. 1989. View Article : Google Scholar : PubMed/NCBI

22 

Steinberg FM, Gershwin ME and Rucker RB: Dietary pyrroloquinoline quinone: Growth and immune response in BALB/c mice. J Nutr. 124:744–753. 1994. View Article : Google Scholar : PubMed/NCBI

23 

Tao R, Karliner JS, Simonis U, Zheng J, Zhang J, Honbo N and Alano CC: Pyrroloquinoline quinone preserves mitochondrial function and prevents oxidative injury in adult rat cardiac myocytes. Biochem Biophys Res Commun. 363:257–262. 2007. View Article : Google Scholar : PubMed/NCBI

24 

Hara H, Hiramatsu H and Adachi T: Pyrroloquinoline quinone is a potentneuroprotective nutrient against 6-hydroxydopamine-induced neurotoxicity. Neurochem Res. 32:489–495. 2007. View Article : Google Scholar : PubMed/NCBI

25 

Xiong XH, Zhao Y, Ge X, Yuan SJ, Wang JH, Zhi JJ, Yang YX, Du BH, Guo WJ, Wang SS, et al: Production and radioprotective effects of pyrroloquinoline quinone. Int J Mol Sci. 12:8913–8923. 2011. View Article : Google Scholar : PubMed/NCBI

26 

Sato K and Toriyama M: Effect of pyrroloquinoline quinone (PQQ) on melanogenic protein expression in murine B16 melanoma. J Dermatol Sci. 53:140–145. 2009. View Article : Google Scholar : PubMed/NCBI

27 

Fadeel B and Orrenius S: Apoptosis: A basic biological phenomenon with wide-ranging implications in human disease. J Intern Med. 258:479–517. 2005. View Article : Google Scholar : PubMed/NCBI

28 

Clarke PG: Developmental cell death: Morphological diversity and multiple mechanisms. Anat Embryol (Berl). 181:195–213. 1990. View Article : Google Scholar : PubMed/NCBI

29 

Tibodeau JD, Benson LM, Isham CR, Owen WG and Bible KC: The Anticanceragent chaetocin is a competitive substrate and inhibitor ofthioredoxin reductase. Antioxid Redox Signal. 11:1097–1106. 2009. View Article : Google Scholar : PubMed/NCBI

30 

Madan E, Prasad S, Roy P, George J and Shukla Y: Regulation of apoptosis by resveratrol through JAK/STAT and mitochondria mediated pathway in human epidermoid carcinoma A431 cells. Biochem Biophys Res Commun. 377:1232–1237. 2008. View Article : Google Scholar : PubMed/NCBI

31 

Liu Z, Huang SL, Li MM, Huang ZS, Lee KS and Gu LQ: Inhibition of thioredoxin reductase by mansonone F analogues: Implications for anticancer activity. Chem Biol Interact. 177:48–57. 2009. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Wen L, Lu X, Wang R, Jin X, Hu L and You C: Pyrroloquinoline quinone induces chondrosarcoma cell apoptosis by increasing intracellular reactive oxygen species. Mol Med Rep 17: 7184-7190, 2018.
APA
Wen, L., Lu, X., Wang, R., Jin, X., Hu, L., & You, C. (2018). Pyrroloquinoline quinone induces chondrosarcoma cell apoptosis by increasing intracellular reactive oxygen species. Molecular Medicine Reports, 17, 7184-7190. https://doi.org/10.3892/mmr.2018.8745
MLA
Wen, L., Lu, X., Wang, R., Jin, X., Hu, L., You, C."Pyrroloquinoline quinone induces chondrosarcoma cell apoptosis by increasing intracellular reactive oxygen species". Molecular Medicine Reports 17.5 (2018): 7184-7190.
Chicago
Wen, L., Lu, X., Wang, R., Jin, X., Hu, L., You, C."Pyrroloquinoline quinone induces chondrosarcoma cell apoptosis by increasing intracellular reactive oxygen species". Molecular Medicine Reports 17, no. 5 (2018): 7184-7190. https://doi.org/10.3892/mmr.2018.8745
Copy and paste a formatted citation
x
Spandidos Publications style
Wen L, Lu X, Wang R, Jin X, Hu L and You C: Pyrroloquinoline quinone induces chondrosarcoma cell apoptosis by increasing intracellular reactive oxygen species. Mol Med Rep 17: 7184-7190, 2018.
APA
Wen, L., Lu, X., Wang, R., Jin, X., Hu, L., & You, C. (2018). Pyrroloquinoline quinone induces chondrosarcoma cell apoptosis by increasing intracellular reactive oxygen species. Molecular Medicine Reports, 17, 7184-7190. https://doi.org/10.3892/mmr.2018.8745
MLA
Wen, L., Lu, X., Wang, R., Jin, X., Hu, L., You, C."Pyrroloquinoline quinone induces chondrosarcoma cell apoptosis by increasing intracellular reactive oxygen species". Molecular Medicine Reports 17.5 (2018): 7184-7190.
Chicago
Wen, L., Lu, X., Wang, R., Jin, X., Hu, L., You, C."Pyrroloquinoline quinone induces chondrosarcoma cell apoptosis by increasing intracellular reactive oxygen species". Molecular Medicine Reports 17, no. 5 (2018): 7184-7190. https://doi.org/10.3892/mmr.2018.8745
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team