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Article Open Access

Adiponectin protects against lung ischemia-reperfusion injury in rats with type 2 diabetes mellitus

  • Authors:
    • Di Li
    • Lin‑Lin Song
    • Juan Wang
    • Chao Meng
    • Xiao‑Guang Cui
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, The Heilongjiang Province Key Laboratory of Research on Anesthesiology and Critical Care Medicine, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 7191-7201
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    Published online on: March 14, 2018
       https://doi.org/10.3892/mmr.2018.8748
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Abstract

Adiponectin (APN) has been associated with the pathogenesis of acute brain, liver and heart injury. However, the role of APN in lung ischemia-reperfusion injury (LIRI) in diabetes mellitus remains unclear. To investigate this, the present study evaluated the effects of APN on lung dysfunction and pathological alterations in rats with type 2 diabetes mellitus via lung ischemia/reperfusion (I/R). The lung‑protective effects of APN globular domain (gAPN) in rats with type 2 diabetes mellitus were also investigated by measuring the oxygenation index, inflammatory cytokines, lung edema, histopathology, oxidative stress, apoptosis and the protein expression levels of phosphorylated 5'adenosine monophosphate‑activated protein kinase (p‑AMPK), endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS). The results of the present study demonstrated that the diabetes mellitus rats + I/R (DIR) group exhibited greater concentrations of tumor necrosis factor‑α and interleukin‑6, and increases in the wet‑weight to dry‑weight ratio, lung injury score, oxidative stress and cellular apoptosis. These effects were accompanied by lower pulmonary oxygenation compared with the normal rat + I/R (NIR) group (P<0.05). Additionally, all of these alterations were attenuated in the NIR + gAPN and DIR + gAPN groups compared with in the NIR and DIR groups, respectively. In the DIR group, the expression levels of p‑AMPK/AMPK and eNOS were significantly downregulated, and the levels of iNOS were upregulated, compared with those of the NIR group. Treatment with APN activated AMPK, increased eNOS expression and attenuated iNOS expression. The results of the present study demonstrated that APN exerted protective effects against LIRI via its anti‑inflammatory, antioxidative stress and anti‑apoptotic activities. These protective effects of APN were eliminated in rats with type 2 diabetes mellitus, in which LIRI was exacerbated. The present study indicated that APN may be a potential therapeutic agent for LIRI in type 2 diabetes mellitus.
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Copy and paste a formatted citation
Spandidos Publications style
Li D, Song LL, Wang J, Meng C and Cui XG: Adiponectin protects against lung ischemia-reperfusion injury in rats with type 2 diabetes mellitus. Mol Med Rep 17: 7191-7201, 2018.
APA
Li, D., Song, L., Wang, J., Meng, C., & Cui, X. (2018). Adiponectin protects against lung ischemia-reperfusion injury in rats with type 2 diabetes mellitus. Molecular Medicine Reports, 17, 7191-7201. https://doi.org/10.3892/mmr.2018.8748
MLA
Li, D., Song, L., Wang, J., Meng, C., Cui, X."Adiponectin protects against lung ischemia-reperfusion injury in rats with type 2 diabetes mellitus". Molecular Medicine Reports 17.5 (2018): 7191-7201.
Chicago
Li, D., Song, L., Wang, J., Meng, C., Cui, X."Adiponectin protects against lung ischemia-reperfusion injury in rats with type 2 diabetes mellitus". Molecular Medicine Reports 17, no. 5 (2018): 7191-7201. https://doi.org/10.3892/mmr.2018.8748
Copy and paste a formatted citation
x
Spandidos Publications style
Li D, Song LL, Wang J, Meng C and Cui XG: Adiponectin protects against lung ischemia-reperfusion injury in rats with type 2 diabetes mellitus. Mol Med Rep 17: 7191-7201, 2018.
APA
Li, D., Song, L., Wang, J., Meng, C., & Cui, X. (2018). Adiponectin protects against lung ischemia-reperfusion injury in rats with type 2 diabetes mellitus. Molecular Medicine Reports, 17, 7191-7201. https://doi.org/10.3892/mmr.2018.8748
MLA
Li, D., Song, L., Wang, J., Meng, C., Cui, X."Adiponectin protects against lung ischemia-reperfusion injury in rats with type 2 diabetes mellitus". Molecular Medicine Reports 17.5 (2018): 7191-7201.
Chicago
Li, D., Song, L., Wang, J., Meng, C., Cui, X."Adiponectin protects against lung ischemia-reperfusion injury in rats with type 2 diabetes mellitus". Molecular Medicine Reports 17, no. 5 (2018): 7191-7201. https://doi.org/10.3892/mmr.2018.8748
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