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Article

Expression of Rv2031c-Rv2626c fusion protein in Mycobacterium smegmatis enhances bacillary survival and modulates innate immunity in macrophages

  • Authors:
    • Hong Jiang
    • Tai‑Lai Luo
    • Jian Kang
    • Zhi‑Kai Xu
    • Li‑Mei Wang
  • View Affiliations / Copyright

    Affiliations: Center for Infectious Diseases, Tangdu Hospital, The Fourth Military Medical University, Xi'an, Shaanxi 710038, P.R. China, Naval Medical Research Institute, Shanghai 200433, P.R. China, Department of Microbiology, School of Basic Medicine, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China
  • Pages: 7307-7312
    |
    Published online on: March 16, 2018
       https://doi.org/10.3892/mmr.2018.8758
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Abstract

Dormancy-associated antigens encoded by the dormancy survival regulon (DosR) genes are required for survival of Mycobacterium tuberculosis (Mtb) in macrophages. However, mechanisms underlying survival of Mtb in macrophages remains to be elucidated. A recombinant Mycobacterium smegmatis strain (rMs) expressing a fusion protein of two dormancy‑associated antigens Rv2031c and Rv2626c from Mtb was constructed in the present study. In an in vitro culture, growth rate of rMs was lower compared with Ms. A total of 24 h following infection of murine macrophages with rMs or Ms, percentage of viable cells decreased and the number of bacteria in viable cells increased compared with Ms, demonstrating that virulence and intracellular survival of rMs were enhanced. Compared with macrophages infected with Ms, necrosis of macrophages infected with rMs was increased, while apoptosis was inhibited. Macrophages infected with rMs secreted more interferon‑γ and interleukin‑6, but fewer nitric oxide and tumor necrosis factor‑α, compared with macrophages infected with Ms. The present study demonstrated that the fusion protein composed of dormancy‑associated antigens Rv2031c and Rv2626c in Ms serves a physiological function of a dormancy‑associated antigen and modulates innate immunity of host macrophages, therefore favoring intracellular bacillary survival.
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Copy and paste a formatted citation
Spandidos Publications style
Jiang H, Luo TL, Kang J, Xu ZK and Wang LM: Expression of Rv2031c-Rv2626c fusion protein in Mycobacterium smegmatis enhances bacillary survival and modulates innate immunity in macrophages. Mol Med Rep 17: 7307-7312, 2018.
APA
Jiang, H., Luo, T., Kang, J., Xu, Z., & Wang, L. (2018). Expression of Rv2031c-Rv2626c fusion protein in Mycobacterium smegmatis enhances bacillary survival and modulates innate immunity in macrophages. Molecular Medicine Reports, 17, 7307-7312. https://doi.org/10.3892/mmr.2018.8758
MLA
Jiang, H., Luo, T., Kang, J., Xu, Z., Wang, L."Expression of Rv2031c-Rv2626c fusion protein in Mycobacterium smegmatis enhances bacillary survival and modulates innate immunity in macrophages". Molecular Medicine Reports 17.5 (2018): 7307-7312.
Chicago
Jiang, H., Luo, T., Kang, J., Xu, Z., Wang, L."Expression of Rv2031c-Rv2626c fusion protein in Mycobacterium smegmatis enhances bacillary survival and modulates innate immunity in macrophages". Molecular Medicine Reports 17, no. 5 (2018): 7307-7312. https://doi.org/10.3892/mmr.2018.8758
Copy and paste a formatted citation
x
Spandidos Publications style
Jiang H, Luo TL, Kang J, Xu ZK and Wang LM: Expression of Rv2031c-Rv2626c fusion protein in Mycobacterium smegmatis enhances bacillary survival and modulates innate immunity in macrophages. Mol Med Rep 17: 7307-7312, 2018.
APA
Jiang, H., Luo, T., Kang, J., Xu, Z., & Wang, L. (2018). Expression of Rv2031c-Rv2626c fusion protein in Mycobacterium smegmatis enhances bacillary survival and modulates innate immunity in macrophages. Molecular Medicine Reports, 17, 7307-7312. https://doi.org/10.3892/mmr.2018.8758
MLA
Jiang, H., Luo, T., Kang, J., Xu, Z., Wang, L."Expression of Rv2031c-Rv2626c fusion protein in Mycobacterium smegmatis enhances bacillary survival and modulates innate immunity in macrophages". Molecular Medicine Reports 17.5 (2018): 7307-7312.
Chicago
Jiang, H., Luo, T., Kang, J., Xu, Z., Wang, L."Expression of Rv2031c-Rv2626c fusion protein in Mycobacterium smegmatis enhances bacillary survival and modulates innate immunity in macrophages". Molecular Medicine Reports 17, no. 5 (2018): 7307-7312. https://doi.org/10.3892/mmr.2018.8758
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