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Effects and mechanism of amyloid β1-42 on mitochondria in astrocytes

  • Authors:
    • Yunyi Yao
    • Jin‑Zhong Huang
    • Yingqi Chen
    • He‑Juan Hu
    • Xiying Tang
    • Xianhong Li
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry, Xuzhou Medical University, Xuzhou, Jiangsu 221004, P.R. China, Department of Neurology, Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu 213000, P.R. China, Department of Neurology, Suzhou Hospital of Traditional Chinese Medicine, Suzhou, Jiangsu 215009, P.R. China, Department of Medical Technology, Suzhou Vocational Health College, Suzhou, Jiangsu 215009, P.R. China
    Copyright: © Yao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 6997-7004
    |
    Published online on: March 16, 2018
       https://doi.org/10.3892/mmr.2018.8761
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Abstract

Amyloid β (Aβ)1-42 is strongly associated with Alzheimer's disease (AD). The effects of Aβ1‑42 on astrocytes remain largely unknown. The present study focused on the effects of Aβ1‑42 on U87 human glioblastoma cells as astrocytes for in vitro investigation and mouse brains for in vivo investigation. The mechanism and regulation of mitochondria and cytochrome P450 reductase (CPR) were also investigated. As determined by MTT assays, low doses of Aβ1‑42 (<1 µM) marginally promoted astrocytosis compared with the 0 µM group within 24 h, however, after 48 h treatment these doses reduced cellular growth compared with the 0 µM group. Furthermore, Aβ1‑42 doses >5 µM inhibited the growth of U87 cells compared with the 0 µM group after 24 and 48 h treatment. Immunofluorescence analysis demonstrated that astrocytosis was also observed in early stage AD mice compared with wild‑type (WT) mice. In addition, concentrations of Aβ1‑42 were also significantly higher in early stage AD mice compared with WT mice, however, the levels were markedly lower compared with later stage AD mice, as determined by ELISA. In addition to increased levels of Aβ1‑42 in mice with later stage AD, reduced astrocyte staining was observed compared with WT mice. Western blotting indicated that the effect of Aβ1‑42 on U87 cell apoptosis may be regulated via Bcl‑2 and caspase‑3 located in mitochondria, whose functions, including adenosine triphosphate generation, electron transport chain and mitochondrial membrane potential, were inhibited by Aβ1‑42. During this process, the expression and activity of cytochrome P450 reductase was also downregulated. The current study provides novel insight into the effects of Aβ1‑42 on astrocytes and highlights a potential role for astrocytes in the protection against AD.
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Copy and paste a formatted citation
Spandidos Publications style
Yao Y, Huang JZ, Chen Y, Hu HJ, Tang X and Li X: Effects and mechanism of amyloid β1-42 on mitochondria in astrocytes. Mol Med Rep 17: 6997-7004, 2018.
APA
Yao, Y., Huang, J., Chen, Y., Hu, H., Tang, X., & Li, X. (2018). Effects and mechanism of amyloid β1-42 on mitochondria in astrocytes. Molecular Medicine Reports, 17, 6997-7004. https://doi.org/10.3892/mmr.2018.8761
MLA
Yao, Y., Huang, J., Chen, Y., Hu, H., Tang, X., Li, X."Effects and mechanism of amyloid β1-42 on mitochondria in astrocytes". Molecular Medicine Reports 17.5 (2018): 6997-7004.
Chicago
Yao, Y., Huang, J., Chen, Y., Hu, H., Tang, X., Li, X."Effects and mechanism of amyloid β1-42 on mitochondria in astrocytes". Molecular Medicine Reports 17, no. 5 (2018): 6997-7004. https://doi.org/10.3892/mmr.2018.8761
Copy and paste a formatted citation
x
Spandidos Publications style
Yao Y, Huang JZ, Chen Y, Hu HJ, Tang X and Li X: Effects and mechanism of amyloid β1-42 on mitochondria in astrocytes. Mol Med Rep 17: 6997-7004, 2018.
APA
Yao, Y., Huang, J., Chen, Y., Hu, H., Tang, X., & Li, X. (2018). Effects and mechanism of amyloid β1-42 on mitochondria in astrocytes. Molecular Medicine Reports, 17, 6997-7004. https://doi.org/10.3892/mmr.2018.8761
MLA
Yao, Y., Huang, J., Chen, Y., Hu, H., Tang, X., Li, X."Effects and mechanism of amyloid β1-42 on mitochondria in astrocytes". Molecular Medicine Reports 17.5 (2018): 6997-7004.
Chicago
Yao, Y., Huang, J., Chen, Y., Hu, H., Tang, X., Li, X."Effects and mechanism of amyloid β1-42 on mitochondria in astrocytes". Molecular Medicine Reports 17, no. 5 (2018): 6997-7004. https://doi.org/10.3892/mmr.2018.8761
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