Ameliorative effect of nicergoline on cognitive function through the PI3K/AKT signaling pathway in mouse models of Alzheimer's disease

  • Authors:
    • Guoyao Zang
    • Lizheng Fang
    • Liying Chen
    • Chenyao Wang
  • View Affiliations

  • Published online on: March 20, 2018     https://doi.org/10.3892/mmr.2018.8786
  • Pages: 7293-7300
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Abstract

Alzheimer's disease is one of the most common age‑associated diseases that frequently leads to memory disorders, cognitive decline and dementia. Evidence suggests that nicergoline serves an important role in the apoptosis of hippocampal cells, memory recovery, cognitive function and neuronal survival. However, the signaling pathway affected by nicergoline treatment remains to be elucidated. The purpose of the present study was to investigate the role of nicergoline in the cognitive competence of a mouse model of Alzheimer's disease. The apoptosis rates of hippocampal cells were studied in mice with Alzheimer's disease treated with nicergoline compared with the negative control. Apoptosis‑associated gene expression levels in hippocampal cells, and hippocampus area, were analyzed in the experimental mice. Visual attention and inhibitory control were assessed and neural counting was performed in brain regions of interest. The phosphatidylinositol 3‑kinase (PI3K)/RAC‑α serine/threonine‑protein kinase (AKT) signaling pathway was additionally analyzed in hippocampal cells following treatment with nicergoline. The results of the present study demonstrated that nicergoline ameliorated apoptosis in hippocampal cells and hippocampus tissue in 3xTg‑AD mice with Alzheimer's disease. The data indicated that apoptosis‑associated genes, including caspase‑3, BCL2 associated X, BH3 interacting domain death agonist and caspase‑9, were downregulated in hippocampal cells isolated from nicergoline-treated experimental mice. In addition, the expression levels of inflammatory factors, in addition to oxidative stress, were decreased in hippocampal cells treated with nicergoline. Additionally, amyloid precursor protein accumulation was cleared in the hippocampal area in nicergoline‑treated mice. Nicergoline inhibited neuronal loss and prevented cognitive impairment through the restoration of learning/memory ability. It was additionally demonstrated in the present study that nicergoline improved motor attention impairment and cognitive competence in hippocampal cells by acting on the PI3K/AKT signaling pathway. Therefore, memory recovery, cognitive function and neuronal survival were repaired by nicergoline via inhibition of the PI3K/AKT signaling pathway, suggesting that nicergoline may be an efficient drug for the clinical treatment of patients with Alzheimer's disease.

Introduction

Senile dementia is a multifactorial syndrome characterized by cognitive functional disorders and cognitive decline (1). Alzheimer's disease is among the most common pathogeneses leading to senile dementia, with a highest incidence rate among the elderly (2,3). Alzheimer's disease is a progressive neurodegenerative syndrome characterized by the presence of neurodegenerative disorders (4). Alzheimer's disease primarily impairs hippocampus-associated memory and cognition, and leads to cognitive functional disorders and impairments in cognition, including memory, language or attention (5,6). Previous studies have suggested that Alzheimer's may directly affect hippocampal cells (79). The association between corticocerebral and hippocampal neuronal apoptosis has been demonstrated in a rat model of Alzheimer's disease and in a clinical study (10,11). Dysfunction and aberrant apoptosis of hippocampalcells has been observed in preclinical and clinical studies (12). Inhibition of neuronal apoptosis may attenuate the phosphorylation of microtubule-associated protein tau (MAPT) and protect memory, which may be beneficial for the treatment of Alzheimer's disease (13,14). Absalon et al (15) suggested that the activation of cell cycle entry, MAPT-phosphorylation and the inhibition of apoptosis in post-mitotic neurons through microRNA-regulated gene expression may contribute to the recovery of mice with Alzheimer's disease. These findings suggested that the apoptosis of hippocampal cells is associated with the progression of cognitive impairment, and thus with the onset and development of Alzheimer's disease.

The apoptosis of hippocampal cells is a diagnostic criterion for Alzheimer's disease (16,17). Decreased apoptosis in the hippocampus signifies an improvement in cognitive impairment. Previous studies have supported the hypothesis that inflammatory responses are the most important pathogenic factors for the initiation and progression of Alzheimer's disease (18,19). Although Alzheimer's disease has been extensively studied, comprehensive assessments have not been performed in an animal model. The majority of research in animal models of Alzheimer's disease has focused on the neuroprotective effects of anti-dementia drugs against inflammation, apoptosis and neuronal excitability (20). Oxidative stress has been associated with Alzheimer's disease and the development of neurodegenerative processes (21). Therefore, the development of pharmacological agents targeting apoptosis, inflammation and oxidative stress represents a novel prospect for the treatment of Alzheimer's disease.

Nicergoline is a derivative of ergot produced by semisynthesis that exhibits the potential to selectively block α-1A adrenergic receptors in the brain and periphery (22). Nicergoline may improve a number of aging-associated diseases, including dysphagia, ischemia and dizziness (2325). Nicergoline is beneficial for the treatment of cognitive impairment (2628). At present, nicergolineis a registered drug in >50 countries and, for the last 30 years, it has been used for the treatment of cognitive impairment and behavioral disorders in the elderly. Therefore, nicergoline may contribute to the treatment of Alzheimer's disease.

In the present study, it was hypothesized that nicergoline may be able to protect neurons against apoptosis by controlling the expression of apoptosis-associated genes. The efficacy of nicergoline in the prevention of cognitive impairment was evaluated in a rat model of Alzheimer's disease and determined by the assessment of the learning memory. The molecular mechanism of nicergoline-mediated improvement in hippocampal cells was studied. Although nicergoline demonstrated neuroprotective effects via stimulation of the phosphatidylinositol 3-kinase (PI3K)/RAC-α serine/threonine-protein kinase (AKT) signaling pathway, further analysis of larger volumes of preclinical data is required to understand the potential efficacy and tolerability.

Materials and methods

Ethical approval

The present study was approved by the Ethics Committee of Zhejiang University (Hangzhou, China). All surgery and euthanasia of experimental mice was performed under sodium pentobarbital anesthesia to minimize suffering.

Cells and reagents

Hippocampal cells were isolated from 3×Tg-AD mice and cultured in 1640 medium (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) supplemented with 10% fetal bovine serum (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA). Hippocampal cells were treated with PI3K inhibitor LY294002 (2 mg/ml) and cultured in for 12 h a 37°C humidified atmosphere with 5% CO2.

Animal study

A total of twenty 6-week-old 3×Tg-AD mice (male, 28–35 g) with the Alzheimer's disease were purchased from Vital River Laboratory Animal Technology Co., Ltd. (Shanghai, China). All animals were housed in a temperature-controlled facility at 23±1°C and relative humidity of 50±5% with a 12-h light/dark cycle with free access to food and water. The mice were divided into two group (n=10 in each group) and given intravenous injections of nicergoline (10 mg/kg body weight) or PBS (10 mg/kg body weight; both from Sigma-Aldrich; Merck KGaA) once a day. The treatment lasted 60 days.

Histological and immunohistochemical analysis

Hippocampal tissues were isolated from the CA1 regions of experimental mice following a 60-day treatment with nicergoline (10 mg/kg body weight) or PBS. The brains were frozen in liquid nitrogen and following perfusion, fixation and cryoprotection, coronal sections were cut to 4 µm in a cryostat. After blocking with 5% Bull Serum Albumin (Sigma-Aldrich; Merck KGaA) for 1 h at 37°C, free-floating sections were rinsed with PBS and placed in a solution with goat anti-mouse amyloid β (Aβ)-42 (1:1,000; cat. no. ab201060; Abcam, Cambridge, UK), Aβ-40 (1:1,000; cat. no. ab17295; Abcam) and amyloid precursor protein (APP; 1:1,000; cat. no. ab32136; Abcam) for 12 h at 4°C. Following incubation the sections were washed and incubated with a secondary rabbit anti-goat antibody conjugated with horseradish peroxidase (1:500; cat. no. A-11034; Chemicon International; Thermo Fisher Scientific, Inc.) for Aβ-42, Aβ-40 and APP staining for 1 h at 37°C. The sections were washed and observed using a fluorescent video microscope (BZ-9000; Keyence Corporation, Osaka, Japan) at magnification ×40. Immunohistochemical staining was used to examine the abundance of neuroprotection-associated proteins in the hippocampus. Immunohistochemical procedures were performed as previously described (29).

Immunofluorescence

Hippocampal CA1 regions were isolated from experimental mice following treatment with nicergoline. Hippocampal neural cells were fixed in situ with bromodeoxyuridine and NeuN for 24 h at 4°C. The impairment of neurogenesis was analyzed under a fluorescence microscope (Olympus BX61; Olympus Corporation, Tokyo, Japan) at magnification 40×.

Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay

ATUNEL assay was performed to analyze apoptotic neurons in the hippocampus as previously described (30). Brain sections were viewed under alight microscope and of apoptotic morphology was labeled in sections. Magnification, ×40.

ELISA analysis

ELISA kits [Huiying Chemical Industry (Xiamen) Co., Ltd., Shanghai, China] were used to determine interleukin (IL)-1 (cat. no. MLB00C; Bio-Rad Laboratories, Inc., Hercules, CA, USA), tumor necrosis factor (TNF)-α (cat. no. MTA00B; Bio-Rad Laboratories, Inc.) and IL-6 (cat. no. M6000B; Bio-Rad Laboratories, Inc.) serum levels in mice. The procedures were performed according to the manufacturer's protocol. The final results were recorded at a wavelength of 450 nm.

Flow cytometry

The apoptosis of hippocampal neural cells was evaluated using an Annexin V-fluorescein isothiocyanate (FITC) Apoptosis Staining/Detection kit (cat. no. ab14085; Abcam, Cambridge, UK) and a propidium iodide (PI) apoptosis detection kit (BD Biosciences, Franklin Lakes, NJ, USA). Hippocampal neural cells were isolated from experimental mice and suspended with Annexin V-FITC and PI, according to the manufacturer's protocols. Fluorescence was measured via fluorescence-activated cell sorting using a flow cytometer (BD Biosciences). Cell apoptosis was analyzed using Expo32-ADC v. 1.2B software (Beckman Coulter, Inc., Brea, CA, USA).

Fluorescent reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis

Total RNA was extracted from experimental mouse hippocampal are as using RNA Easy Mini Extract kit (Sigma-Aldrich; Merck KGaA) and the RNA served as a template for cDNA synthesis by RT reaction using a PrimeScript RT Master mix kit (Takara Biotechnology Co., Ltd., Dalian, China). A total of one-tenth of the cDNA was used in the fluorescent RT-qPCR reaction using iQ SYBR-Green (Invitrogen; Thermo Fisher Scientific, Inc.). The PCR conditions included an initial denaturation step of 94°C for 2 min, followed by 30 cycles of 94°C for 30 sec, 59°C for 30 sec, 72°C for 2 min and a final elongation step at 72°C for 10 min. All forward and reverse primers were summarized in Table I. Relative mRNA expression was calculated by the 2−ΔΔCq calculation (31). Results were presented as a fold difference relative to the housekeeping β-actin control gene.

Table I.

Sequences of primers used in this study.

Table I.

Sequences of primers used in this study.

Sequence

Gene nameReverseForward
P53 5′-TTAAGCTTTTTGCGTTCGGGCTGGGAGC-3′ 5′-ATGGTGGCATGAACCTGTGG-3′
Bcl-2 5′-CGTCATAACTAAAGACACCCC-3′ 5′-TTCATCTCCAGTATCCGACTC-3′
Caspase-3 5′-ATGGAGAACAACAAAACCTCAGT-3′ 5′-TTGCTCCCATGTATGGTCTTTAC-3′
Caspase-9 5′-GCCCTTGCCTCTGAGTAGTG-3′ 5′-CCAACCAAATGAAGCCAAGT-3′
Bax 5′-CACCAATCACCTGCGGTACA-3′ 5′-CAGATCACGTCATCGCAC-3′
Bid 5′-CGACGAGGTGAAGACATCCT-3′ 3′-AGCAGAGATGGTGCATGACT-3′
β-actin 5′-CCTTCCTGGGCATGGAGTCCT-3′ 5′-GGAGCAATGATCTTGATCTTC-3′

[i] Bcl-2, B-cell lymphoma-2; Bax, B-cell lymphoma-2 associated X-protein; Bid, BH3 interacting-domain death agonist.

Western blotting

Hippocampal cells were homogenized in radioimmunoprecipitation assay lysis buffer containing protease inhibitor (Sigma-Aldrich; Merck KGaA) and centrifuged at 6,000 × g at 4°C for 10 min. The supernatant was used for the analysis of target proteins using a protein extraction kit (Qiagen Sciences, Inc., Gaithersburg, MD, USA), according to manufacturer's protocol. The protein concentration was measured in samples by a bicinchoninic acid protein assay kit (Thermo Fisher Scientific, Inc.). Protein samples (10 µg) were separated on 12.5% SDS-PAGE gels and transferred onto polyvinylidene difluoride membranes (EMD Millipore, Billerica, MA, USA). SDS-PAGE was performed as previously described (32). For western blotting, primary antibodies: IL-1β (1:400; cat. no. ab200478), IL-6, TNF-α (1:500; cat. no. ab1793), Foxp2 (1:400; cat. no. ab16046), Src homology 2-containing inositol phosphatase (SxIP; 1:400; cat. no. ab45142), EB (1:400; cat. no. ab157217), P53 (1:400; cat. no. ab26), Bcl-2 (1:400; cat. no. ab32124), caspase-3 (1:500; cat. no. ab217), caspase-9 (1:500; cat. no. ab52298), Bid (1:400; cat. no. ab62469), Bax (1:400; cat. no. ab32124), Neprilysin (1:500; cat. no. ab79423), insulin (1:500; cat. no. ab32216), insulin-like growth factor-binding protein 3 (IGFBP-3; 1:500; cat. no. ab75988), vascular endothelial growth factor β (VEGF-β; 1:500; cat. no. ab53463), superoxide dismutase (SOD; 1:500; cat. no. ab13533), catalase (CAT; 1:500; cat. no. ab16731), glutathione (GSH; 1:500; cat. no. ab26255), PI3K (1:1,000; cat. no. ab182651; Thermo Fisher Scientific, Inc.), AKT (1:1,000; cat. no. ab8933; Thermo Fisher Scientific, Inc.), pAKT (1:1,000; cat. no. ab18260; Thermo Fisher Scientific, Inc.), ROS (1:1,000; cat. no. PA5-14732; Thermo Fisher Scientific, Inc.) and β-actin (1:2,000, cat. no. ab8226) were added following blocking with 5% skimmed milk for 1 h at 37°C, then the membranes incubated with primary antibodies overnight at 4°C and then incubated with secondary antibodies (1:5,000; PV-6001; OriGene Technologies, Inc., Rockville, MD, USA)for 1 h at 37°C. Finally, protein bands were visualized using WesternBright Enhanced Chemiluminescent substrate (Advansta, Inc., Menlo Park, CA, USA).

Cognitive and behavioral tests

The cognitive competence of mice was determined by measuring the open field activity levels of mice in black plexiglass boxes (60×60×25 cm), to analyze the therapeutic effects of nicergoline. Mice were placed in open black boxes for 10 min and the behavior of the mice was monitored and evaluated using an auto-tracking system (EthoVision 8.0; Noldus, Wageningen, the Netherlands). A Morris water maze test was performed prior to and post-treatment with nicergoline to measure cognitive function. The Morris water maze experiment was performed three times in a circular stainless steel tank (155 cm diameter, 60 cm depth) filled with water to a depth of 40 cm (27.0±1.0°C) that was made opaque by the addition of skimmed milk. Mice learned to find a hidden circular platform (10 cm diameter, 1 cm below the surface of the water) in a fixed area in one quadrant of the tank.

Thionin staining

Brain sections (30 µm thick) were prepared and stained with thionin for 2 h at room temperature. Images of stained tissues were digitally captured with an Olympus IX71 microscope controlled by DP manager software (Olympus Corporation, Tokyo, Japan) at a magnification of ×1.5.

Statistical analyses

All data are presented as the mean ± standard error of the mean. Statistical significance was determined using a two-tailed Student's t-test with SPSS 19.0 (IBM Corp., Armonk, NY, USA). One-way analysis of variance followed by Tukey's post hoc test, Kaplan-Meier analysis and log-rank statistical analysis were performed using GraphPad software version 5.0 (GraphPad Software, Inc., La Jolla, CA, USA). P<0.05 was considered to indicate a statistically significant difference.

Results

Nicergoline improves impaired neurogenesis and cognitive competence in mice with Alzheimer's disease

In order to analyze the efficacy of nicergolinein the treatment of Alzheimer's disease, mice with Alzheimer's disease received treatment with nicergoline (10 mg/kg body weight) or were administered PBS as a control. It was determined that nicergoline treatment markedly improved neurogenesis and cognitive competence compared with the control (Fig. 1A and B). In mice that received treatment with nicergoline, the degree of dementia was decreased following a 60-day course of treatment (Fig. 1C). Immunological staining indicated that pathogenic Aβ-42 and −40 peptides and APP were downregulated in the hippocampi of experimental mice (Fig. 1D). Levels of the neuroprotective forkhead box protein P2 (Foxp2), Src homology 2-containing inositol phosphatase (SxIP) and end-binding proteins (EB) were increased in hippocampi of nicergoline-treated mice (Fig. 1E). Additionally, marked differences in the dispersion of the pyramidal cell layer were observed between the nicergoline-treated and control groups, as determined by thionin staining (Fig. 1F). These results suggested that nicergoline exerted beneficial effects on neurogenesis and cognitive competence in mice with Alzheimer's disease.

Nicergoline inhibits apoptosis in hippocampal cells from mice with Alzheimer's disease

As previously indicated, apoptosis of hippocampal cells is ubiquitous in patients with Alzheimer's disease (33). Therefore, the present study examined the apoptosis of hippocampal cells in a mouse model of Alzheimer's disease following treatment with nicergoline or PBS. It was demonstrated that nicergoline inhibited apoptosis in hippocampal cells isolated from mice with Alzheimer's disease and analyzed by flow cytometry in vitro (Fig. 2A). The TUNEL assay demonstrated that apoptotic hippocampal cells were significantly less prevalent in nicergoline-treated mice in vivo (Fig. 2B). In addition, the expression levels of apoptotic factors in hippocampal cells isolated from nicergoline-treated mice were investigated. The mRNA and protein levels of pro-apoptotic caspase-3, BCL2 associated X (Bax), BH3 interacting domain death agonist (Bid) and caspase-9 were downregulated in the hippocampal cells of nicergoline-treated mice (Fig. 2C and D). However, the mRNA and protein levels of anti-apoptotic p53 and apoptosis regulator Bcl-2 were upregulated in the hippocampal cells of nicergoline-treated mice (Fig. 2E and F). The results of the present study indicated that nicergoline may inhibit apoptosis in hippocampal cells by regulating the activation of apoptosis-associated genes.

Nicergoline inhibits inflammation and oxidative stress in hippocampal cells from mice with Alzheimer's disease

The expression levels of inflammatory factors in hippocampal cells from experimental mice were examined. It was demonstrated that the plasma concentrations of IL-1, IL-6 and TNF-α were decreased in hippocampal cells treated with nicergoline (Fig. 3A-C). Neprilysin and insulin expression levels were upregulated in hippocampal cells in the mice with Alzheimer's disease treated with nicergoline for 60 days (Fig. 3D). In addition, insulin-like growth factor-binding protein 3 and vascular endothelial growth factor β protein levels were increased in hippocampal cells in the experimental mice treated with nicergoline (Fig. 3E). Oxidative stress differences between experimental mice we analyzed following treatment with nicergoline. As presented in Fig. 3F, the expression levels of reactive oxygen species, superoxide dismutase and glutathione were downregulated in hippocampal cells in the experimental mice treated with nicergoline. The results of the present study indicated that nicergoline mitigated Alzheimer's disease in mice via inhibition of inflammation and oxidative stress in hippocampal cells.

Nicergoline regulates the activity of hippocampal cells through the PI3K/AKT signaling pathway

In order to elucidate the nicergoline-mediated mitigation of Alzheimer's disease in mice, molecular alterations in the PI3K/AKT signaling pathway in hippocampal cells were studied. As presented in Fig. 4A, treatment with nicergoline significantly increased the expression of PI3K and AKT in hippocampal cells isolated from experimental mice. In addition, nicergoline significantly promoted the phosphorylation of AKT compared with the negative control (Fig. 4B). It was observed that inhibition of PI3K activity (PI3KI) by LY294002 obviated the AKT expression and phosphorylation stimulated by treatment with nicergoline (Nicergoline + PI3KI; NiPI3KI) in hippocampal cells (Fig. 4C). In addition, the expression levels of IL-1, IL-6 and TNF-α were upregulated in PI3K-inhibitedhippocampal cells in vitro (Fig. 4D). Additionally, oxidative stress was increased in PI3K-inhibited hippocampal cells in vitro (Fig. 4E). Notably, the apoptosis rate was increased in PI3K inhibitor-treated hippocampal cells in vitro (Fig. 4F). The aforementioned results demonstrated that nicergoline regulated the activity of hippocampal cells through stimulation of the PI3K/AKT signaling pathway.

Discussion

In the present study, the effect of nicergoline on Alzheimer's disease and the mechanism of action of nicergoline were analyzed. The results of the present study contributed to the efforts to develop an effective treatment for Alzheimer's disease. Nicergoline is as an efficient drug for the treatment of the cranial nerve symptoms associated with dementia, although there is no available data on its molecular mechanism of action in Alzheimer's disease. The PI3K/AKT pathway has been hypothesized to mediate the nicergoline-induced therapeutic effect on Alzheimer's disease in a mouse model. The results of the present study confirmed that nicergoline regulated PI3K/AKT expression and phosphorylation in the hippocampus of mice with Alzheimer's disease. The findings of the present study indicated that nicergoline inhibited inflammation, apoptosis and oxidative stress through upregulation of the PI3K/AKT signaling pathway.

The PI3K/AKT signaling pathway serves an essential role in cell growth, proliferation and survival under physiological conditions (34). There is scarce evidence of the association between the PI3K/AKT signaling pathway and Alzheimer's disease. Kong et al (35) reported that nicorandil serves a neuroprotective role in a cellular model of Alzheimer's disease through activation of the PI3K/AKT signaling pathway. Kitagishi et al (36) demonstrated that the PI3K/AKT/glycogen synthase kinase-3 β pathway is involved in cell signaling in neuronal cells affected by Alzheimer's disease. In addition, ginseng induced a neuroprotective effect on D-galactose/aluminum chloride via the PI3K/AKT signaling pathway in the hippocampus, improved memory and reduced the content of Aβ-42 and MAPT in rats with Alzheimer's disease (37). Additionally, following treatment with salidroside, the PI3K/AKT signaling pathway ameliorated toxicity in Aβ-treated primary brain neural cultures (38). These previous reports suggested that neuroprotection is associated with the activity of the PI3K/AKT signaling pathway in hippocampal cells in cellular and animals models of Alzheimer's disease. The results of the present study indicated that nicergoline was able to improve neurogenesis and cognitive competence through upregulation of the PI3K/AKT signaling pathway and inhibition of inflammation, apoptosis and oxidative stress in mice with Alzheimer's disease.

Alzheimer's disease is characterized by apoptosis of hippocampal cells, senile plaques, oxidative stress, neurofibrillary tangles and inflammation (39). Chronic inflammatory responses stimulate the progression of Alzheimer's disease (40). Quinn et al (41) studied the association between inflammation and cerebral amyloidosis in an animal model of Alzheimer's disease. Balez et al (20) reported that apigenin may induce neuroprotective effects in a pluripotent stem cell model of Alzheimer's disease via inhibition of inflammation, improvement of neuronal excitability and reduced apoptosis. The results of the present study demonstrated that nicergoline was able to reduce inflammation via downregulation of inflammatory factors in hippocampal cells. Downregulation of inflammation contributes to reduced apoptosis in hippocampal cells. In addition, chronic inflammatory responses and oxidative stress are associated with the expression of Aβ protein during the progression of Alzheimer's disease (42). The results of the present study suggested that nicergoline was able to improve Aβ protein accumulation in the hippocampi of experimental mice. Additionally, apoptosis, a ubiquitous biological phenomenon in cells, is genetically controlled. Bcl-2 and caspase-3 are two key molecular apoptosis regulators and their function in the regulation of brain cell apoptosis was previously reported (4346). One study demonstrated that downregulation of Bcl-2 and simultaneous upregulation of Bax and Bid activated p53 and caspase-3, and consequently induced neuronal apoptosis in the rat hippocampus (47). Bcl-2 and caspase-3 are two important molecular regulators of apoptosis and overexpression of Bcl-2 inhibited the activation of caspase-3 and apoptosis (48). Therefore, the data presented in the present study may contribute to the understanding of the therapeutic properties of nicergoline for the treatment of Alzheimer's disease.

In conclusion, the results of the present preclinical study suggested that nicergoline may be an effective drug for the treatment of impaired neurogenesis and cognitive competence in Alzheimer's disease. Treatment with nicergoline markedly improved visual attention, inhibitory control and the neural cell count via inhibition of apoptosis in hippocampal cells and prevention of the expression of inflammatory mediators in mice with Alzheimer's disease. Notably, nicergoline-mediated activation of the PI3K/AKT signaling pathway was investigated in mice with Alzheimer's disease. The results suggested that nicergoline regulated neurogenesis and cognitive competence through the PI3K/AKT signaling pathway in the hippocampal cells of experimental mice. In conclusion, the results of the present study suggested that nicergoline may be a promising drug for the treatment of Alzheimer's disease.

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May-2018
Volume 17 Issue 5

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Spandidos Publications style
Zang G, Fang L, Chen L and Wang C: Ameliorative effect of nicergoline on cognitive function through the PI3K/AKT signaling pathway in mouse models of Alzheimer's disease. Mol Med Rep 17: 7293-7300, 2018
APA
Zang, G., Fang, L., Chen, L., & Wang, C. (2018). Ameliorative effect of nicergoline on cognitive function through the PI3K/AKT signaling pathway in mouse models of Alzheimer's disease. Molecular Medicine Reports, 17, 7293-7300. https://doi.org/10.3892/mmr.2018.8786
MLA
Zang, G., Fang, L., Chen, L., Wang, C."Ameliorative effect of nicergoline on cognitive function through the PI3K/AKT signaling pathway in mouse models of Alzheimer's disease". Molecular Medicine Reports 17.5 (2018): 7293-7300.
Chicago
Zang, G., Fang, L., Chen, L., Wang, C."Ameliorative effect of nicergoline on cognitive function through the PI3K/AKT signaling pathway in mouse models of Alzheimer's disease". Molecular Medicine Reports 17, no. 5 (2018): 7293-7300. https://doi.org/10.3892/mmr.2018.8786