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Article

IL-10 inhibits retinal pigment epithelium cell proliferation and migration through regulation of VEGF in rhegmatogenous retinal detachment

  • Authors:
    • Quiqing Zhao
    • Mingli Ji
    • Xuemei Wang
  • View Affiliations / Copyright

    Affiliations: Department of Ophthalmology, The Affiliated Hospital of Yan'an University, Yan'an, Shaanxi 716000, P.R. China, Department of Ophthalmology, The Second Affiliated Hospital of Xi'an Medical University, Xi'an, Shaanxi 710004, P.R. China, Department of Ophthalmology, The Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine, Xianyang, Shaanxi 712046, P.R. China
  • Pages: 7301-7306
    |
    Published online on: March 20, 2018
       https://doi.org/10.3892/mmr.2018.8787
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Abstract

Rhegmatogenous retinal detachment (RRD) is a disorder of the eye that affects physical and mental health. Retinal pigment epithelium (RPE) is closely associated with RRD, and it is hypothesized that RPE-secreted inflammatory cytokines may induce early pathological changes of RRD and may participate in RPE proliferation and migration. The present study determined a role for interleukin (IL)‑10 as an RPE‑secreted immune regulatory factor that contributes to RRD. A rat RRD model was established and RPE cells were isolated and cultivated. RPE cells were randomly divided into four groups, three treated with different concentrations of IL‑10 (100, 50, and 20 mM) and one untreated. RPE cell proliferation was evaluated by MTT assay and the activity of caspase‑3 was also measured. RPE cell invasion was determined by Transwell assay. Vascular endothelial growth factor A (VEGF) expression was examined by reverse transcription‑quantitative polymerase chain reaction and western blotting; IL‑1 and IL‑6 levels were measured by ELISA. IL‑10 treatment suppressed RPE cell proliferation and migration, promoted caspase‑3 activity, inhibited VEGF mRNA and protein expression, and downregulated the secretion of inflammatory cytokines IL‑1 and IL‑6 in RRD group compared with the untreated Model group. The aforementioned effects of IL‑10 became more evident with increasing IL‑10 concentration. IL‑10 suppressed inflammation, facilitated RPE cell apoptosis and inhibited cell proliferation and migration through the regulation of VEGF expression.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao Q, Ji M and Wang X: IL-10 inhibits retinal pigment epithelium cell proliferation and migration through regulation of VEGF in rhegmatogenous retinal detachment. Mol Med Rep 17: 7301-7306, 2018.
APA
Zhao, Q., Ji, M., & Wang, X. (2018). IL-10 inhibits retinal pigment epithelium cell proliferation and migration through regulation of VEGF in rhegmatogenous retinal detachment. Molecular Medicine Reports, 17, 7301-7306. https://doi.org/10.3892/mmr.2018.8787
MLA
Zhao, Q., Ji, M., Wang, X."IL-10 inhibits retinal pigment epithelium cell proliferation and migration through regulation of VEGF in rhegmatogenous retinal detachment". Molecular Medicine Reports 17.5 (2018): 7301-7306.
Chicago
Zhao, Q., Ji, M., Wang, X."IL-10 inhibits retinal pigment epithelium cell proliferation and migration through regulation of VEGF in rhegmatogenous retinal detachment". Molecular Medicine Reports 17, no. 5 (2018): 7301-7306. https://doi.org/10.3892/mmr.2018.8787
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao Q, Ji M and Wang X: IL-10 inhibits retinal pigment epithelium cell proliferation and migration through regulation of VEGF in rhegmatogenous retinal detachment. Mol Med Rep 17: 7301-7306, 2018.
APA
Zhao, Q., Ji, M., & Wang, X. (2018). IL-10 inhibits retinal pigment epithelium cell proliferation and migration through regulation of VEGF in rhegmatogenous retinal detachment. Molecular Medicine Reports, 17, 7301-7306. https://doi.org/10.3892/mmr.2018.8787
MLA
Zhao, Q., Ji, M., Wang, X."IL-10 inhibits retinal pigment epithelium cell proliferation and migration through regulation of VEGF in rhegmatogenous retinal detachment". Molecular Medicine Reports 17.5 (2018): 7301-7306.
Chicago
Zhao, Q., Ji, M., Wang, X."IL-10 inhibits retinal pigment epithelium cell proliferation and migration through regulation of VEGF in rhegmatogenous retinal detachment". Molecular Medicine Reports 17, no. 5 (2018): 7301-7306. https://doi.org/10.3892/mmr.2018.8787
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