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Integrated analysis of B‑cell and T‑cell receptors by high‑throughput sequencing reveals conserved repertoires in IgA nephropathy

  • Authors:
    • Minglin Ou
    • Fengping Zheng
    • Xinzhou Zhang
    • Song Liu
    • Donge Tang
    • Peng Zhu
    • Jingjun Qiu
    • Yong Dai
  • View Affiliations / Copyright

    Affiliations: Clinical Medical Research Center of Second Clinical Medical College, Jinan University, Shenzhen People's Hospital, Shenzhen, Guangdong 518020, P.R. China, Lab Center of Shenzhen Pingshan People's Hospital, Shenzhen, Guangdong 518118, P.R. China
    Copyright: © Ou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 7027-7036
    |
    Published online on: March 20, 2018
       https://doi.org/10.3892/mmr.2018.8793
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Abstract

Immunoglobulin A nephropathy (IgAN) is a type of glomerular disorder associated with immune dysregulation, and understanding B‑/T‑cell receptors (BCRs/TCRs) may be valuable for the development of specific immunotherapeutic interventions. In the present study, B and T cells were isolated from IgAN patients and healthy controls, and the composition of the BCR/TCR complementarity‑determining region (CDR)3 was analyzed by multiplex polymerase chain reaction, high‑throughput sequencing and bioinformatics. The present results revealed that the BCR/TCR CDR3 clones were expressed at very low frequencies, and the composition of clone types in patients with IgAN was skewed; the majority of clones were unique, and only 12 BCR and 228 TCR CDR3 clones were public ones, of which 16 were expressed at a significantly higher frequency in patients with IgAN (P<0.001). There were also certain conserved amino acid residues between unique clones or groups, and the residues GMDV, EQY and EQF were recurring only in the IgAN group. In addition, some VDJ gene recombinations indicated great variation between groups, including 4 high‑frequency VDJ gene recombinations in the IgAN patients (P<0.001). Immune repertoires provide novel information, and conserved BCR/TCR CDR3 clones and VDJ gene recombinations with great variation may be potential therapeutic targets for IgAN patients.
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Copy and paste a formatted citation
Spandidos Publications style
Ou M, Zheng F, Zhang X, Liu S, Tang D, Zhu P, Qiu J and Dai Y: Integrated analysis of B‑cell and T‑cell receptors by high‑throughput sequencing reveals conserved repertoires in IgA nephropathy. Mol Med Rep 17: 7027-7036, 2018.
APA
Ou, M., Zheng, F., Zhang, X., Liu, S., Tang, D., Zhu, P. ... Dai, Y. (2018). Integrated analysis of B‑cell and T‑cell receptors by high‑throughput sequencing reveals conserved repertoires in IgA nephropathy. Molecular Medicine Reports, 17, 7027-7036. https://doi.org/10.3892/mmr.2018.8793
MLA
Ou, M., Zheng, F., Zhang, X., Liu, S., Tang, D., Zhu, P., Qiu, J., Dai, Y."Integrated analysis of B‑cell and T‑cell receptors by high‑throughput sequencing reveals conserved repertoires in IgA nephropathy". Molecular Medicine Reports 17.5 (2018): 7027-7036.
Chicago
Ou, M., Zheng, F., Zhang, X., Liu, S., Tang, D., Zhu, P., Qiu, J., Dai, Y."Integrated analysis of B‑cell and T‑cell receptors by high‑throughput sequencing reveals conserved repertoires in IgA nephropathy". Molecular Medicine Reports 17, no. 5 (2018): 7027-7036. https://doi.org/10.3892/mmr.2018.8793
Copy and paste a formatted citation
x
Spandidos Publications style
Ou M, Zheng F, Zhang X, Liu S, Tang D, Zhu P, Qiu J and Dai Y: Integrated analysis of B‑cell and T‑cell receptors by high‑throughput sequencing reveals conserved repertoires in IgA nephropathy. Mol Med Rep 17: 7027-7036, 2018.
APA
Ou, M., Zheng, F., Zhang, X., Liu, S., Tang, D., Zhu, P. ... Dai, Y. (2018). Integrated analysis of B‑cell and T‑cell receptors by high‑throughput sequencing reveals conserved repertoires in IgA nephropathy. Molecular Medicine Reports, 17, 7027-7036. https://doi.org/10.3892/mmr.2018.8793
MLA
Ou, M., Zheng, F., Zhang, X., Liu, S., Tang, D., Zhu, P., Qiu, J., Dai, Y."Integrated analysis of B‑cell and T‑cell receptors by high‑throughput sequencing reveals conserved repertoires in IgA nephropathy". Molecular Medicine Reports 17.5 (2018): 7027-7036.
Chicago
Ou, M., Zheng, F., Zhang, X., Liu, S., Tang, D., Zhu, P., Qiu, J., Dai, Y."Integrated analysis of B‑cell and T‑cell receptors by high‑throughput sequencing reveals conserved repertoires in IgA nephropathy". Molecular Medicine Reports 17, no. 5 (2018): 7027-7036. https://doi.org/10.3892/mmr.2018.8793
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