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Identification of Streptococcus mitis321A vaccine antigens based on reverse vaccinology

  • Authors:
    • Qiao Zhang
    • Kexiong Lin
    • Changzheng Wang
    • Zhi Xu
    • Li Yang
    • Qianli Ma
  • View Affiliations / Copyright

    Affiliations: Institute of Respiratory Disease, Xinqiao Hospital of Third Military Medical University, Chongqing 400037, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 7477-7486
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    Published online on: March 28, 2018
       https://doi.org/10.3892/mmr.2018.8799
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Abstract

Streptococcus mitis (S. mitis) may transform into highly pathogenic bacteria. The aim of the present study was to identify potential antigen targets for designing an effective vaccine against the pathogenic S. mitis321A. The genome of S. mitis321A was sequenced using an Illumina Hiseq2000 instrument. Subsequently, Glimmer 3.02 and Tandem Repeat Finder (TRF) 4.04 were used to predict genes and tandem repeats, respectively, with DNA sequence function analysis using the Basic Local Alignment Search Tool (BLAST) in the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Cluster of Orthologous Groups of proteins (COG) databases. Putative gene antigen candidates were screened with BLAST ahead of phylogenetic tree analysis. The DNA sequence assembly size was 2,110,680 bp with 40.12% GC, 6 scaffolds and 9 contig. Consequently, 1,944 genes were predicted, and 119 TRF, 56 microsatellite DNA, 10 minisatellite DNA and 154 transposons were acquired. The predicted genes were associated with various pathways and functions concerning membrane transport and energy metabolism. Multiple putative genes encoding surface proteins, secreted proteins and virulence factors, as well as essential genes were determined. The majority of essential genes belonged to a phylogenetic lineage, while 321AGL000129 and 321AGL000299 were on the same branch. The current study provided useful information regarding the biological function of the S. mitis321A genome and recommends putative antigen candidates for developing a potent vaccine against S. mitis.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang Q, Lin K, Wang C, Xu Z, Yang L and Ma Q: Identification of Streptococcus mitis321A vaccine antigens based on reverse vaccinology. Mol Med Rep 17: 7477-7486, 2018.
APA
Zhang, Q., Lin, K., Wang, C., Xu, Z., Yang, L., & Ma, Q. (2018). Identification of Streptococcus mitis321A vaccine antigens based on reverse vaccinology. Molecular Medicine Reports, 17, 7477-7486. https://doi.org/10.3892/mmr.2018.8799
MLA
Zhang, Q., Lin, K., Wang, C., Xu, Z., Yang, L., Ma, Q."Identification of Streptococcus mitis321A vaccine antigens based on reverse vaccinology". Molecular Medicine Reports 17.6 (2018): 7477-7486.
Chicago
Zhang, Q., Lin, K., Wang, C., Xu, Z., Yang, L., Ma, Q."Identification of Streptococcus mitis321A vaccine antigens based on reverse vaccinology". Molecular Medicine Reports 17, no. 6 (2018): 7477-7486. https://doi.org/10.3892/mmr.2018.8799
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Q, Lin K, Wang C, Xu Z, Yang L and Ma Q: Identification of Streptococcus mitis321A vaccine antigens based on reverse vaccinology. Mol Med Rep 17: 7477-7486, 2018.
APA
Zhang, Q., Lin, K., Wang, C., Xu, Z., Yang, L., & Ma, Q. (2018). Identification of Streptococcus mitis321A vaccine antigens based on reverse vaccinology. Molecular Medicine Reports, 17, 7477-7486. https://doi.org/10.3892/mmr.2018.8799
MLA
Zhang, Q., Lin, K., Wang, C., Xu, Z., Yang, L., Ma, Q."Identification of Streptococcus mitis321A vaccine antigens based on reverse vaccinology". Molecular Medicine Reports 17.6 (2018): 7477-7486.
Chicago
Zhang, Q., Lin, K., Wang, C., Xu, Z., Yang, L., Ma, Q."Identification of Streptococcus mitis321A vaccine antigens based on reverse vaccinology". Molecular Medicine Reports 17, no. 6 (2018): 7477-7486. https://doi.org/10.3892/mmr.2018.8799
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