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Article Open Access

TRPM2 promotes the proliferation and invasion of pancreatic ductal adenocarcinoma

  • Authors:
    • Rui Lin
    • Yufeng Wang
    • Quanning Chen
    • Zhongyan Liu
    • Shuai Xiao
    • Bingyi Wang
    • Baomin Shi
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, P.R. China
    Copyright: © Lin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 7537-7544
    |
    Published online on: March 28, 2018
       https://doi.org/10.3892/mmr.2018.8816
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Abstract

The aim of the present study was to investigate transient receptor potential cation channel subfamily M member 2 (TRPM2), a promising therapeutic target and biomarker for pancreatic ductal adenocarcinoma (PDAC) prognosis, in addition to determining its effects regarding tumor progression and invasion. PDAC is a fatal disease with a poor prognosis, and its associated pathogenic molecular mechanisms remain to be determined. In the present study, combined analysis using genomic and transcriptomic data from two PDAC studies was performed to discover a survival‑associated biomarker of PDAC. Survival analysis for genes mutated in ≥10 patients was performed using a Kaplan‑Meier curve and tested for significance using a log‑rank test. Furthermore, gene‑expression correlation analysis was performed to determine the genes with the strongest correlations to TRPM2. In addition, a Cell Counting Kit‑8 assay, a scratch wound‑healing assay and a Transwell assay were performed in the present study to investigate the proliferative, invasive and metastatic ability of PANC‑1 cells in TRPM2‑overexpressing and downregulated groups. The mutated TRPM2 gene had a strong negative correlation with patient survival probability compared with the normal control group (P=1.06x10‑4). Expression of TRPM2 was strongly correlated with expression of probable phospholipid‑transporting ATPase IM, γ‑parvin, tudor domain containing 9, Toll‑like receptor 7 and Scm‑like with four MBT domains protein 2 according to the criterion of a correlation coefficient >0.5. Furthermore, the results of the present study demonstrated that the TRPM2 overexpression in a PDAC cell line (PANC‑1) promoted cell proliferation, invasion and metastatic ability. TRPM2 represents a potential therapeutic target and prognostic marker for patients with PDAC. TRPM2 regulates cell proliferation, invasion and migration; however, the underlying mechanism requires further investigation in future studies.
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Copy and paste a formatted citation
Spandidos Publications style
Lin R, Wang Y, Chen Q, Liu Z, Xiao S, Wang B and Shi B: TRPM2 promotes the proliferation and invasion of pancreatic ductal adenocarcinoma. Mol Med Rep 17: 7537-7544, 2018.
APA
Lin, R., Wang, Y., Chen, Q., Liu, Z., Xiao, S., Wang, B., & Shi, B. (2018). TRPM2 promotes the proliferation and invasion of pancreatic ductal adenocarcinoma. Molecular Medicine Reports, 17, 7537-7544. https://doi.org/10.3892/mmr.2018.8816
MLA
Lin, R., Wang, Y., Chen, Q., Liu, Z., Xiao, S., Wang, B., Shi, B."TRPM2 promotes the proliferation and invasion of pancreatic ductal adenocarcinoma". Molecular Medicine Reports 17.6 (2018): 7537-7544.
Chicago
Lin, R., Wang, Y., Chen, Q., Liu, Z., Xiao, S., Wang, B., Shi, B."TRPM2 promotes the proliferation and invasion of pancreatic ductal adenocarcinoma". Molecular Medicine Reports 17, no. 6 (2018): 7537-7544. https://doi.org/10.3892/mmr.2018.8816
Copy and paste a formatted citation
x
Spandidos Publications style
Lin R, Wang Y, Chen Q, Liu Z, Xiao S, Wang B and Shi B: TRPM2 promotes the proliferation and invasion of pancreatic ductal adenocarcinoma. Mol Med Rep 17: 7537-7544, 2018.
APA
Lin, R., Wang, Y., Chen, Q., Liu, Z., Xiao, S., Wang, B., & Shi, B. (2018). TRPM2 promotes the proliferation and invasion of pancreatic ductal adenocarcinoma. Molecular Medicine Reports, 17, 7537-7544. https://doi.org/10.3892/mmr.2018.8816
MLA
Lin, R., Wang, Y., Chen, Q., Liu, Z., Xiao, S., Wang, B., Shi, B."TRPM2 promotes the proliferation and invasion of pancreatic ductal adenocarcinoma". Molecular Medicine Reports 17.6 (2018): 7537-7544.
Chicago
Lin, R., Wang, Y., Chen, Q., Liu, Z., Xiao, S., Wang, B., Shi, B."TRPM2 promotes the proliferation and invasion of pancreatic ductal adenocarcinoma". Molecular Medicine Reports 17, no. 6 (2018): 7537-7544. https://doi.org/10.3892/mmr.2018.8816
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