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Article

Knockdown of peroxiredoxin V increases glutamate‑induced apoptosis in HT22 hippocampal neuron cells

  • Authors:
    • Gui‑Nan Shen
    • Lei Liu
    • Li Feng
    • Yu Jin
    • Mei‑Hua Jin
    • Ying‑Hao Han
    • Cheng‑Hao Jin
    • Yong‑Zhe Jin
    • Dong‑Soek Lee
    • Tae Ho Kwon
    • Yu‑Dong Cui
    • Hu‑Nan Sun
  • View Affiliations / Copyright

    Affiliations: Department of Disease Model Animal Research Center, College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, P.R. China, Pharmaron Beijing Co., Ltd., Beijing 100176, P.R. China, Laboratory of Anatomy and Histology, Yanbian University Health Science Center, Yanji, Jilin 133000, P.R. China, Laboratory of Molecular Neurobiology, School of Life Sciences, KNU Creative Bio Research Group (BK21 Plus Project), Kyungpook National University, Daegu 41566, Republic of Korea, Laboratory of Animal Genetic Engineering and Stem Cell Biology, Subtropical/Tropical Organism Gene Bank, Jeju National University, Jeju, Jeju 63243, Republic of Korea
  • Pages: 7827-7834
    |
    Published online on: March 29, 2018
       https://doi.org/10.3892/mmr.2018.8826
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Abstract

High concentrations of glutamate may mediate neuronal cell apoptosis by increasing intracellular reactive oxygen species (ROS) levels. Peroxiredoxin V (Prx V), a member of the Prx family, serves crucial roles in protecting cells from oxidative stress. The present study investigated the regulatory effect of Prx V on glutamate‑induced effects on viability and apoptosis in HT22 cells. Western blotting was used for protein expression analysis and Annexin V/PI staining and flow cytometry for determination of apoptosis. The results demonstrated that glutamate may ROS‑dependently increase HT22 cell apoptosis and upregulate Prx V protein levels. Furthermore, knockdown of Prx V protein expression with a lentivirus significantly enhanced HT22 cell apoptosis mediated by glutamate, which was reversed by inhibition of ROS with N‑acetyl‑L‑cysteine. Inhibiting the extracellular signal‑regulated kinase (ERK) signaling pathway with PD98059, a specific inhibitor for ERK phosphorylation, markedly decreased glutamate‑induced HT22 cell apoptosis in Prx V knockdown cells, indicating the potential involvement of ERK signaling in glutamate‑induced HT22 cell apoptosis. In addition, an increase in nuclear apoptosis‑inducing factor was observed in Prx V knockdown HT22 cells following glutamate treatment, compared with mock cells, whereas no differences in B‑cell lymphoma‑2 and cleaved‑caspase‑3 protein expression levels were observed between mock and Prx V knockdown cells. The results of the present study indicated that Prx V may have potential as a therapeutic molecular target for glutamate‑induced neuronal cell death and provide novel insight into the role of Prx V in oxidative‑stress induced neuronal cell death.
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Copy and paste a formatted citation
Spandidos Publications style
Shen GN, Liu L, Feng L, Jin Y, Jin MH, Han YH, Jin CH, Jin YZ, Lee DS, Kwon T, Kwon T, et al: Knockdown of peroxiredoxin V increases glutamate‑induced apoptosis in HT22 hippocampal neuron cells. Mol Med Rep 17: 7827-7834, 2018.
APA
Shen, G., Liu, L., Feng, L., Jin, Y., Jin, M., Han, Y. ... Sun, H. (2018). Knockdown of peroxiredoxin V increases glutamate‑induced apoptosis in HT22 hippocampal neuron cells. Molecular Medicine Reports, 17, 7827-7834. https://doi.org/10.3892/mmr.2018.8826
MLA
Shen, G., Liu, L., Feng, L., Jin, Y., Jin, M., Han, Y., Jin, C., Jin, Y., Lee, D., Kwon, T., Cui, Y., Sun, H."Knockdown of peroxiredoxin V increases glutamate‑induced apoptosis in HT22 hippocampal neuron cells". Molecular Medicine Reports 17.6 (2018): 7827-7834.
Chicago
Shen, G., Liu, L., Feng, L., Jin, Y., Jin, M., Han, Y., Jin, C., Jin, Y., Lee, D., Kwon, T., Cui, Y., Sun, H."Knockdown of peroxiredoxin V increases glutamate‑induced apoptosis in HT22 hippocampal neuron cells". Molecular Medicine Reports 17, no. 6 (2018): 7827-7834. https://doi.org/10.3892/mmr.2018.8826
Copy and paste a formatted citation
x
Spandidos Publications style
Shen GN, Liu L, Feng L, Jin Y, Jin MH, Han YH, Jin CH, Jin YZ, Lee DS, Kwon T, Kwon T, et al: Knockdown of peroxiredoxin V increases glutamate‑induced apoptosis in HT22 hippocampal neuron cells. Mol Med Rep 17: 7827-7834, 2018.
APA
Shen, G., Liu, L., Feng, L., Jin, Y., Jin, M., Han, Y. ... Sun, H. (2018). Knockdown of peroxiredoxin V increases glutamate‑induced apoptosis in HT22 hippocampal neuron cells. Molecular Medicine Reports, 17, 7827-7834. https://doi.org/10.3892/mmr.2018.8826
MLA
Shen, G., Liu, L., Feng, L., Jin, Y., Jin, M., Han, Y., Jin, C., Jin, Y., Lee, D., Kwon, T., Cui, Y., Sun, H."Knockdown of peroxiredoxin V increases glutamate‑induced apoptosis in HT22 hippocampal neuron cells". Molecular Medicine Reports 17.6 (2018): 7827-7834.
Chicago
Shen, G., Liu, L., Feng, L., Jin, Y., Jin, M., Han, Y., Jin, C., Jin, Y., Lee, D., Kwon, T., Cui, Y., Sun, H."Knockdown of peroxiredoxin V increases glutamate‑induced apoptosis in HT22 hippocampal neuron cells". Molecular Medicine Reports 17, no. 6 (2018): 7827-7834. https://doi.org/10.3892/mmr.2018.8826
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