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Article Open Access

Ubiquitin‑like protein FAT10 regulates DNA damage repair via modification of proliferating cell nuclear antigen

  • Authors:
    • Zhenchuan Chen
    • Wei Zhang
    • Zhimin Yun
    • Xue Zhang
    • Feng Gong
    • Yunfang Wang
    • Shouping Ji
    • Ling Leng
  • View Affiliations / Copyright

    Affiliations: Stem Cell and Tissue Engineering Lab, Beijing Institute of Transfusion Medicine, Beijing 100850, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 7487-7496
    |
    Published online on: April 5, 2018
       https://doi.org/10.3892/mmr.2018.8843
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Abstract

In response to DNA damage, proliferating cell nuclear antigen (PCNA) has an important role as a positive regulator and as a scaffold protein associated with DNA damage bypass and repair pathways by serving as a platform for the recruitment of associated components. As demonstrated in the present study, the ubiquitin‑like modifier human leukocyte antigen F locus adjacent transcript 10 (FAT10), which binds to PCNA but has not previously been demonstrated to be associated with the DNA damage response (DDR), is induced by ultraviolet/ionizing radiation and VP‑16 treatment in HeLa cells. Furthermore, DNA damage enhances FAT10 expression. Immunoprecipitation analysis suggested PCNA is modified by FAT10, and the degradation of FATylated PCNA located in the cytoplasm is regulated by the 26S proteasome, which is also responsible for the upregulation of nuclear foci formation. Furthermore, immunofluorescence experiment suggested FAT10 co‑localizes with PCNA in nuclear foci, thus suggesting that FATylation of PCNA may affect DDR via the induction of PCNA degradation in the cytoplasm or nucleus. In addition, immunohistochemistry experiment suggested the expression levels of FAT10 and PCNA are enhanced in HCC tissues compared with healthy liver tissues; however, the expression of FAT10 is suppressed in regenerated liver tissues, which express high levels of PCNA, thus suggesting that the association between FAT10 and PCNA expression is only exhibited in tumor tissues. In conclusion, the results of the present study suggest that FAT10 may be involved in DDR and therefore the progression of tumorigenesis.
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Copy and paste a formatted citation
Spandidos Publications style
Chen Z, Zhang W, Yun Z, Zhang X, Gong F, Wang Y, Ji S and Leng L: Ubiquitin‑like protein FAT10 regulates DNA damage repair via modification of proliferating cell nuclear antigen. Mol Med Rep 17: 7487-7496, 2018.
APA
Chen, Z., Zhang, W., Yun, Z., Zhang, X., Gong, F., Wang, Y. ... Leng, L. (2018). Ubiquitin‑like protein FAT10 regulates DNA damage repair via modification of proliferating cell nuclear antigen. Molecular Medicine Reports, 17, 7487-7496. https://doi.org/10.3892/mmr.2018.8843
MLA
Chen, Z., Zhang, W., Yun, Z., Zhang, X., Gong, F., Wang, Y., Ji, S., Leng, L."Ubiquitin‑like protein FAT10 regulates DNA damage repair via modification of proliferating cell nuclear antigen". Molecular Medicine Reports 17.6 (2018): 7487-7496.
Chicago
Chen, Z., Zhang, W., Yun, Z., Zhang, X., Gong, F., Wang, Y., Ji, S., Leng, L."Ubiquitin‑like protein FAT10 regulates DNA damage repair via modification of proliferating cell nuclear antigen". Molecular Medicine Reports 17, no. 6 (2018): 7487-7496. https://doi.org/10.3892/mmr.2018.8843
Copy and paste a formatted citation
x
Spandidos Publications style
Chen Z, Zhang W, Yun Z, Zhang X, Gong F, Wang Y, Ji S and Leng L: Ubiquitin‑like protein FAT10 regulates DNA damage repair via modification of proliferating cell nuclear antigen. Mol Med Rep 17: 7487-7496, 2018.
APA
Chen, Z., Zhang, W., Yun, Z., Zhang, X., Gong, F., Wang, Y. ... Leng, L. (2018). Ubiquitin‑like protein FAT10 regulates DNA damage repair via modification of proliferating cell nuclear antigen. Molecular Medicine Reports, 17, 7487-7496. https://doi.org/10.3892/mmr.2018.8843
MLA
Chen, Z., Zhang, W., Yun, Z., Zhang, X., Gong, F., Wang, Y., Ji, S., Leng, L."Ubiquitin‑like protein FAT10 regulates DNA damage repair via modification of proliferating cell nuclear antigen". Molecular Medicine Reports 17.6 (2018): 7487-7496.
Chicago
Chen, Z., Zhang, W., Yun, Z., Zhang, X., Gong, F., Wang, Y., Ji, S., Leng, L."Ubiquitin‑like protein FAT10 regulates DNA damage repair via modification of proliferating cell nuclear antigen". Molecular Medicine Reports 17, no. 6 (2018): 7487-7496. https://doi.org/10.3892/mmr.2018.8843
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