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Article Open Access

Calotropin regulates the apoptosis of non‑small cell cancer by regulating the cytotoxic T‑lymphocyte associated antigen 4‑mediated TGF‑β/ERK signaling pathway

  • Authors:
    • Lu Tian
    • Xiao‑Hong Xie
    • Ze‑Hao Zhu
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    Affiliations: Department of Respiratory Medicine, The Fourth People's Hospital of Guiyang, Guiyang, Guizhou 550002, P.R. China, Department of Respiratory Diseases, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Institute of Respiratory Disease, Guangzhou, Guangdong 510120, P.R. China, Department of Respiratory Medicine, The Affiliated Zhoushan Hospital of Wenzhou Medical University, Zhoushan, Zhejiang 316000, P.R. China
    Copyright: © Tian et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 7683-7691
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    Published online on: April 5, 2018
       https://doi.org/10.3892/mmr.2018.8853
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Abstract

Non‑small‑cell lung cancer (NSCLC) is one of the most common malignancies that is responsible for a high level of cancer‑associated mortalities worldwide. Previous evidence has shown that Calotropin is an upstream activator of protein kinase B, which can further inhibit the growth and promote the apoptosis of NSCLC cells. In the present study, the efficacy of Calotropin on growth, aggressiveness and apoptosis of NSCLC cells was investigated, as well as the potential underlying mechanism. The results demonstrated that Calotropin inhibited H358 cell growth, migration and invasion. Flow cytometry assay showed that Calotropin promoted the apoptosis of H358 cells in vitro. Western blot analysis demonstrated that Calotropin inhibited fibronectin (FN), Vimentin (VIM) and E‑cadherin (Eca) protein expression levels in H358 cells in vitro. In addition, Calotropin treatment upregulated pro‑apoptosis gene expression, including caspase‑3, caspase‑8 and apoptotic protease activating factor‑1, and downregulated anti‑apoptosis gene expression, including P53, B‑cell lymphoma (Bcl) 2 and Bcl‑2‑like protein 2 in H358 cells. The results also revealed that the expression levels of cytotoxic T‑lymphocyte associated antigen 4 (CTLA‑4) were decreased by Calotropin treatment in H358 cells. Analyses of the underlying mechanism indicated that Calotropin inhibited transforming growth factor‑β (TGF‑β) and extracellular signal‑regulated kinase (ERK) expression. Overexpression of CTLA‑4 inhibited Calotropin‑mediated downregulation of TGF‑β and ERK expression in H358 cells. In vivo assay revealed that Calotropin administration significantly inhibited tumor growth and prolonged animal survival over the 120‑day observation period. Immunohistochemistry demonstrated that the number of apoptotic cells increased and the expression levels of CTLA‑4 were decreased in the Calotropin‑treated tumor group when compared with control. In addition, the expression levels of TGF‑β and ERK were downregulated in the Calotropin‑treated tumor group compared with control. In conclusion, the results of the present study indicated that Calotropin administration regulated NSCLC apoptosis by downregulating the CTLA‑4‑mediated TGF‑β/ERK signaling pathway, suggesting that Calotropin may be a potential anti‑cancer agent for the treatment of NSCLC.
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1 

Paesmans M, Grigoriu B, Ocak S, Roelandts M, Lafitte JJ, Holbrechts S, Berghmans T, Meert AP, Moretti L, Danyi S, et al: Systematic qualitative review of randomised trials conducted in nonsmall cell lung cancer with a noninferiority or equivalence design. Eur Respir J. 45:511–524. 2015. View Article : Google Scholar : PubMed/NCBI

2 

D'Antonio C, Milano A, Righini R, Onesti CE, Bassanelli M, Falcone R, Paris I, Lauro S and Marchetti P: Pharmacogenomics in lung cancer chemotherapy: A review of what the oncologist should know. Anticancer Res. 34:5241–5250. 2014.PubMed/NCBI

3 

Brody H: Lung cancer. Nature. 513:S12014. View Article : Google Scholar : PubMed/NCBI

4 

Moro-Sibilot D, Smit E, de Castro Carpeño J, Lesniewski-Kmak K, Aerts JG, Villatoro R, Kraaij K, Nacerddine K, Dyachkova Y, Smith KT, et al: Non-small cell lung cancer patients with brain metastases treated with first-line platinum-doublet chemotherapy: Analysis from the European FRAME study. Lung Cancer. 90:427–432. 2015. View Article : Google Scholar : PubMed/NCBI

5 

Barnett SA, Downey RJ, Zheng J, Plourde G, Shen R, Chaft J, Akhurst T, Park BJ and Rusch VW: Utility of routine PET imaging to predict response and survival after induction therapy for non-small cell lung cancer. Ann Thorac Surg. 101:1052–1059. 2016. View Article : Google Scholar : PubMed/NCBI

6 

Fenton-Ambrose L and Kazerooni EA: Preventative care: Lung-cancer screens now worth the cost. Nature. 514:352014. View Article : Google Scholar : PubMed/NCBI

7 

Sawabata N, Nagayasu T, Kadota Y, Goto T, Horio H, Mori T, Yamashita S and Iwasaki A: Risk assessment of lung resection for lung cancer according to pulmonary function: Republication of systematic review and proposals by guideline committee of the Japanese association for chest surgery 2014. Gen Thorac Cardiovasc Surg. 63:14–21. 2015. View Article : Google Scholar : PubMed/NCBI

8 

Sekiguchi Y, Shimada A, Imai H, Wakabayashi M, Sugimoto K, Nakamura N, Sawada T, Komatsu N and Noguchi M: Patient with refractory multiple myeloma developing eosinophilia after lenalidomide treatment and lung cancer 9 months later: Case report and review of the literature. Indian J Hematol Blood Transfus. 30 Suppl 1:S264–S270. 2014. View Article : Google Scholar

9 

Schild SE, Rule WG, Ashman JB, Vora SA, Keole S, Anand A, Liu W and Bues M: Proton beam therapy for locally advanced lung cancer: A review. World J Clin Oncol. 5:568–575. 2014. View Article : Google Scholar : PubMed/NCBI

10 

Kato T, Fujii T, Ide M, Takada T, Sutoh T, Morita H, Yajima R, Yamaguchi S, Tsutsumi S, Asao T, et al: Effect of long interval between hyperthermochemoradiation therapy and surgery for rectal cancer on apoptosis, proliferation and tumor response. Anticancer Res. 34:3141–3146. 2014.PubMed/NCBI

11 

Mo EP, Zhang RR, Xu J, Zhang H, Wang XX, Tan QT, Liu FL, Jiang RW and Cai SH: Calotropin from Asclepias curasavica induces cell cycle arrest and apoptosis in cisplatin-resistant lung cancer cells. Biochem Biophys Res Commun. 478:710–715. 2016. View Article : Google Scholar : PubMed/NCBI

12 

Park HY, Toume K, Arai MA, Sadhu SK, Ahmed F and Ishibashi M: Calotropin: A cardenolide from calotropis gigantea that inhibits Wnt signaling by increasing casein kinase 1alpha in colon cancer cells. Chembiochem. 15:872–878. 2014. View Article : Google Scholar : PubMed/NCBI

13 

Wang SC, Lu MC, Chen HL, Tseng HI, Ke YY, Wu YC and Yang PY: Cytotoxicity of calotropin is through caspase activation and downregulation of anti-apoptotic proteins in K562 cells. Cell Biol Int. 33:1230–1236. 2009. View Article : Google Scholar : PubMed/NCBI

14 

Davey G and Wu Z: Attitudes in China toward the use of animals in laboratory research. Altern Lab Anim. 35:313–316. 2007.PubMed/NCBI

15 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

16 

Renshaw A and Elsheikh TM: A validation study of the Focalpoint GS imaging system for gynecologic cytology screening. Cancer Cytopathol. 121:737–738. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Zhuang T, Djemil T, Qi P, Magnelli A, Stephans K, Videtic G and Xia P: Dose calculation differences between Monte Carlo and pencil beam depend on the tumor locations and volumes for lung stereotactic body radiation therapy. J Appl Clin Med Phys. 14:40112013. View Article : Google Scholar : PubMed/NCBI

18 

Kalyuzhny AE: Combination of TUNEL assay with immunohistochemistry for simultaneous detection of DNA fragmentation and oxidative cell damage. Methods Mol Biol. 682:15–27. 2011. View Article : Google Scholar : PubMed/NCBI

19 

Gharavi N and El-Kadi AO: Expression of cytochrome P450 in lung tumor. Curr Drug Metab. 5:203–210. 2004. View Article : Google Scholar : PubMed/NCBI

20 

Song W, Ma Y, Wang J, Brantley-Sieders D and Chen J: JNK signaling mediates EPHA2-dependent tumor cell proliferation, motility, and cancer stem cell-like properties in non-small cell lung cancer. Cancer Res. 74:2444–2454. 2014. View Article : Google Scholar : PubMed/NCBI

21 

Magnuson WJ, Yeung JT, Guillod PD, Gettinger SN, Yu JB and Chiang VL: Impact of deferring radiation therapy in patients with epidermal growth factor receptor-mutant non-small cell lung cancer who develop brain metastases. Int J Radiat Oncol Biol Phys. 95:673–679. 2016. View Article : Google Scholar : PubMed/NCBI

22 

Jiang SY, Zhao J, Wang MZ, Huo Z, Zhang J, Zhong W and Xu Y: Small-cell lung cancer transformation in patients with pulmonary adenocarcinoma: A case report and review of literature. Medicine (Baltimore). 95:e27522016. View Article : Google Scholar : PubMed/NCBI

23 

Shivapurkar N, Reddy J, Chaudhary PM and Gazdar AF: Apoptosis and lung cancer: A review. J Cell Biochem. 88:885–898. 2003. View Article : Google Scholar : PubMed/NCBI

24 

Drebert Z, MacAskill M, Doughty-Shenton D, De Bosscher K, Bracke M, Hadoke PWF and Beck IM: Colon cancer-derived myofibroblasts increase endothelial cell migration by glucocorticoid-sensitive secretion of a pro-migratory factor. Vascul Pharmacol. 89:19–30. 2017. View Article : Google Scholar : PubMed/NCBI

25 

Kim CW, Go RE, Lee HM, Hwang KA, Lee K, Kim B, Lee MY and Choi KC: Cigarette smoke extracts induced the colon cancer migration via regulating epithelial mesenchymal transition and metastatic genes in human colon cancer cells. Environ Toxicol. 32:690–704. 2017. View Article : Google Scholar : PubMed/NCBI

26 

Klyushnenkova EN, Riabov VB, Kouiavskaia DV, Wietsma A, Zhan M and Alexander RB: Breaking immune tolerance by targeting CD25+ regulatory T cells is essential for the anti-tumor effect of the CTLA-4 blockade in an HLA-DR transgenic mouse model of prostate cancer. Prostate. 74:1423–1432. 2014. View Article : Google Scholar : PubMed/NCBI

27 

Duraiswamy J, Freeman GJ and Coukos G: Dual blockade of PD-1 and CTLA-4 combined with tumor vaccine effectively restores T-cell rejection function in tumors-response. Cancer Res. 74:633–635. 2014. View Article : Google Scholar : PubMed/NCBI

28 

Son CH, Bae JH, Shin DY, Lee HR, Choi YJ, Jo WS, Jung Ho M, Kang CD, Yang K and Park YS: CTLA-4 blockade enhances antitumor immunity of intratumoral injection of immature dendritic cells into irradiated tumor in a mouse colon cancer model. J Immunother. 37:1–7. 2014. View Article : Google Scholar : PubMed/NCBI

29 

Gardner D, Jeffery LE and Sansom DM: Understanding the CD28/CTLA-4 (CD152) pathway and its implications for costimulatory blockade. Am J Transplant. 14:1985–1991. 2014. View Article : Google Scholar : PubMed/NCBI

30 

Li F, Guo Z, Yu H, Zhang X, Si T, Liu C, Yang X and Qi L: Anti-tumor immunological response induced by cryoablation and anti-CTLA-4 antibody in an in vivo RM-1 cell prostate cancer murine model. Neoplasma. 61:659–671. 2014. View Article : Google Scholar : PubMed/NCBI

31 

Slattery ML, Herrick JS, Lundgreen A and Wolff RK: Genetic variation in the TGF-β signaling pathway and colon and rectal cancer risk. Cancer Epidemiol Biomarkers Prev. 20:57–69. 2011. View Article : Google Scholar : PubMed/NCBI

32 

Slattery ML, Lundgreen A, Herrick JS, Caan BJ, Potter JD and Wolff RK: Associations between genetic variation in RUNX1, RUNX2, RUNX3, MAPK1 and eIF4E and riskof colon and rectal cancer: Additional support for a TGF-β-signaling pathway. Carcinogenesis. 32:318–326. 2011. View Article : Google Scholar : PubMed/NCBI

33 

Becker C, Fantini MC and Neurath MF: TGF-beta as a T cell regulator in colitis and colon cancer. Cytokine Growth Factor Rev. 17:97–106. 2006. View Article : Google Scholar : PubMed/NCBI

34 

Becker C, Fantini MC, Schramm C, Lehr HA, Wirtz S, Nikolaev A, Burg J, Strand S, Kiesslich R, Huber S, et al: TGF-beta suppresses tumor progression in colon cancer by inhibition of IL-6 trans-signaling. Immunity. 21:491–501. 2004. View Article : Google Scholar : PubMed/NCBI

35 

Kuchta-Noctor AM, Murray BA, Stanton C, Devery R and Kelly PM: Anticancer activity of buttermilk against SW480 colon cancer cells is associated with caspase-independent cell death and attenuation of Wnt, Akt, and ERK signaling. Nutr Cancer. 68:1234–1246. 2016. View Article : Google Scholar : PubMed/NCBI

36 

Wang CY, Guo ST, Wang JY, Yan XG, Farrelly M, Zhang YY, Liu F, Yari H, La T, Lei FX, et al: Reactivation of ERK and Akt confers resistance of mutant BRAF colon cancer cells to the HSP90 inhibitor AUY922. Oncotarget. 7:49597–49610. 2016.PubMed/NCBI

37 

Beck SE, Jung BH, Del Rosario E, Gomez J and Carethers JM: BMP-induced growth suppression in colon cancer cells is mediated by p21WAF1 stabilization and modulated by RAS/ERK. Cell Signal. 19:1465–1472. 2007. View Article : Google Scholar : PubMed/NCBI

38 

Grijelmo C, Rodrigue C, Svrcek M, Bruyneel E, Hendrix A, de Wever O and Gespach C: Proinvasive activity of BMP-7 through SMAD4/src-independent and ERK/Rac/JNK-dependent signaling pathways in colon cancer cells. Cell Signal. 19:1722–1732. 2007. View Article : Google Scholar : PubMed/NCBI

39 

de Oca J, Azuara D, Sanchez-Santos R, Navarro M, Capella G, Moreno V, Sola A, Hotter G, Biondo S, Osorio A, et al: Caspase-3 activity, response to chemotherapy and clinical outcome in patients with colon cancer. Int J Colorectal Dis. 23:21–27. 2008. View Article : Google Scholar : PubMed/NCBI

40 

Vadde R, Radhakrishnan S, Reddivari L and Vanamala JK: Triphala extract suppresses proliferation and induces apoptosis in human colon cancer stem cells via suppressing c-Myc/Cyclin D1 and elevation of Bax/Bcl-2 ratio. Biomed Res Int. 2015:6492632015. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Tian L, Xie XH and Zhu ZH: Calotropin regulates the apoptosis of non‑small cell cancer by regulating the cytotoxic T‑lymphocyte associated antigen 4‑mediated TGF‑β/ERK signaling pathway. Mol Med Rep 17: 7683-7691, 2018.
APA
Tian, L., Xie, X., & Zhu, Z. (2018). Calotropin regulates the apoptosis of non‑small cell cancer by regulating the cytotoxic T‑lymphocyte associated antigen 4‑mediated TGF‑β/ERK signaling pathway. Molecular Medicine Reports, 17, 7683-7691. https://doi.org/10.3892/mmr.2018.8853
MLA
Tian, L., Xie, X., Zhu, Z."Calotropin regulates the apoptosis of non‑small cell cancer by regulating the cytotoxic T‑lymphocyte associated antigen 4‑mediated TGF‑β/ERK signaling pathway". Molecular Medicine Reports 17.6 (2018): 7683-7691.
Chicago
Tian, L., Xie, X., Zhu, Z."Calotropin regulates the apoptosis of non‑small cell cancer by regulating the cytotoxic T‑lymphocyte associated antigen 4‑mediated TGF‑β/ERK signaling pathway". Molecular Medicine Reports 17, no. 6 (2018): 7683-7691. https://doi.org/10.3892/mmr.2018.8853
Copy and paste a formatted citation
x
Spandidos Publications style
Tian L, Xie XH and Zhu ZH: Calotropin regulates the apoptosis of non‑small cell cancer by regulating the cytotoxic T‑lymphocyte associated antigen 4‑mediated TGF‑β/ERK signaling pathway. Mol Med Rep 17: 7683-7691, 2018.
APA
Tian, L., Xie, X., & Zhu, Z. (2018). Calotropin regulates the apoptosis of non‑small cell cancer by regulating the cytotoxic T‑lymphocyte associated antigen 4‑mediated TGF‑β/ERK signaling pathway. Molecular Medicine Reports, 17, 7683-7691. https://doi.org/10.3892/mmr.2018.8853
MLA
Tian, L., Xie, X., Zhu, Z."Calotropin regulates the apoptosis of non‑small cell cancer by regulating the cytotoxic T‑lymphocyte associated antigen 4‑mediated TGF‑β/ERK signaling pathway". Molecular Medicine Reports 17.6 (2018): 7683-7691.
Chicago
Tian, L., Xie, X., Zhu, Z."Calotropin regulates the apoptosis of non‑small cell cancer by regulating the cytotoxic T‑lymphocyte associated antigen 4‑mediated TGF‑β/ERK signaling pathway". Molecular Medicine Reports 17, no. 6 (2018): 7683-7691. https://doi.org/10.3892/mmr.2018.8853
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