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Article

Silencing of casein kinase 2 inhibits PKC‑induced cell invasion by targeting MMP‑9 in MCF‑7 cells

  • Authors:
    • Jeong‑Mi Kim
    • Eun‑Mi Noh
    • Hyun‑Kyung Song
    • Yong‑Ouk You
    • Sung Hoo Jung
    • Jong‑Suk Kim
    • Kang‑Beom Kwon
    • Young‑Rae Lee
    • Hyun Jo Youn
  • View Affiliations / Copyright

    Affiliations: Center for Metabolic Function Regulation, Wonkwang University School of Medicine, Iksan, Jeollabuk 570‑749, Republic of Korea, Department of Oral Biochemistry, School of Dentistry, Wonkwang University, Iksan, Jeollabuk 570‑749, Republic of Korea, Department of Surgery, Division of Breast and Thyroid Surgery, Chonbuk National University Medical School, Jeonju, Jeollabuk 560‑182, Republic of Korea, Department of Biochemistry, Institute of Medical Science, Chonbuk National University Medical School, Jeonju, Jeollabuk 560‑182, Republic of Korea, Department of Korean Physiology, Wonkwang University School of Korean Medicine, Iksan, Jeonbuk 570‑749, Republic of Korea
  • Pages: 8397-8402
    |
    Published online on: April 13, 2018
       https://doi.org/10.3892/mmr.2018.8885
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Abstract

Casein kinase 2 (CK2) is a serine/threonine protein kinase that has been considered to represent an important factor in mammary tumorigenesis. Increased expression of matrix metalloproteinase‑9 (MMP‑9) via nuclear factor‑κB (NF‑κB) activation has been demonstrated to promote breast cancer cell invasion. In the present study, the involvement of CK2 in protein kinase C (PKC) induced cell invasion in MCF‑7 breast cancer cells was investigated as well as the underlying molecular mechanisms. The mRNA and protein levels of MMP‑9 in MCF‑7 cells were investigated using reverse transcription‑quantitative polymerase chain reaction, western blot analyses and a zymography assay. Cell invasiveness was investigated using a Matrigel invasion assay, and it was revealed that small interfering RNA specific for CK2 suppressed PKC induced cell invasion by regulating MMP‑9 expression via activation of the p38 kinase/c‑Jun N‑terminal kinase/NF‑κB pathway. In addition, it was demonstrated that CK2 inhibitors [apigenin (20 µM), emodin (20 µM) or 2‑dimethylamino‑4,5,6,7‑tetrabromo‑1H‑benzimidazole (2 µM)] suppressed PKC induced cell invasion and MMP‑9 expression. The results of the present study suggested that CK2 is an important factor involved in the induction of MCF‑7 breast cancer cell invasion by PKC. Therefore, CK2 may represent novel candidates for therapy intended to inhibit invasion in breast cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Kim JM, Noh EM, Song HK, You YO, Jung S, Kim JS, Kwon KB, Lee YR and Youn H: Silencing of casein kinase 2 inhibits PKC‑induced cell invasion by targeting MMP‑9 in MCF‑7 cells. Mol Med Rep 17: 8397-8402, 2018.
APA
Kim, J., Noh, E., Song, H., You, Y., Jung, S., Kim, J. ... Youn, H. (2018). Silencing of casein kinase 2 inhibits PKC‑induced cell invasion by targeting MMP‑9 in MCF‑7 cells. Molecular Medicine Reports, 17, 8397-8402. https://doi.org/10.3892/mmr.2018.8885
MLA
Kim, J., Noh, E., Song, H., You, Y., Jung, S., Kim, J., Kwon, K., Lee, Y., Youn, H."Silencing of casein kinase 2 inhibits PKC‑induced cell invasion by targeting MMP‑9 in MCF‑7 cells". Molecular Medicine Reports 17.6 (2018): 8397-8402.
Chicago
Kim, J., Noh, E., Song, H., You, Y., Jung, S., Kim, J., Kwon, K., Lee, Y., Youn, H."Silencing of casein kinase 2 inhibits PKC‑induced cell invasion by targeting MMP‑9 in MCF‑7 cells". Molecular Medicine Reports 17, no. 6 (2018): 8397-8402. https://doi.org/10.3892/mmr.2018.8885
Copy and paste a formatted citation
x
Spandidos Publications style
Kim JM, Noh EM, Song HK, You YO, Jung S, Kim JS, Kwon KB, Lee YR and Youn H: Silencing of casein kinase 2 inhibits PKC‑induced cell invasion by targeting MMP‑9 in MCF‑7 cells. Mol Med Rep 17: 8397-8402, 2018.
APA
Kim, J., Noh, E., Song, H., You, Y., Jung, S., Kim, J. ... Youn, H. (2018). Silencing of casein kinase 2 inhibits PKC‑induced cell invasion by targeting MMP‑9 in MCF‑7 cells. Molecular Medicine Reports, 17, 8397-8402. https://doi.org/10.3892/mmr.2018.8885
MLA
Kim, J., Noh, E., Song, H., You, Y., Jung, S., Kim, J., Kwon, K., Lee, Y., Youn, H."Silencing of casein kinase 2 inhibits PKC‑induced cell invasion by targeting MMP‑9 in MCF‑7 cells". Molecular Medicine Reports 17.6 (2018): 8397-8402.
Chicago
Kim, J., Noh, E., Song, H., You, Y., Jung, S., Kim, J., Kwon, K., Lee, Y., Youn, H."Silencing of casein kinase 2 inhibits PKC‑induced cell invasion by targeting MMP‑9 in MCF‑7 cells". Molecular Medicine Reports 17, no. 6 (2018): 8397-8402. https://doi.org/10.3892/mmr.2018.8885
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