Age‑dependent decreases in insulin‑like growth factor‑I and its receptor expressions in the gerbil olfactory bulb

Lee,Tae‑Kyeong; Chen,Bai Hui; Lee,Jae‑Chul; Shin,Myoung Cheol; Cho,Jun Hwi; Lee,Hyang‑Ah; Choi,Jung Hoon; Hwang,In Koo; Kang,Il Jun; Ahn,Ji Hyeon; Park,Joon Ha; Choi,Soo Young; Won,Moo‑Ho

doi:10.3892/mmr.2018.8886

Age‑dependent decreases in insulin‑like growth factor‑I and its receptor expressions in the gerbil olfactory bulb

Authors:
- Tae‑Kyeong Lee
- Bai Hui Chen
- Jae‑Chul Lee
- Myoung Cheol Shin
- Jun Hwi Cho
- Hyang‑Ah Lee
- Jung Hoon Choi
- In Koo Hwang
- Il Jun Kang
- Ji Hyeon Ahn
- Joon Ha Park
- Soo Young Choi
- Moo‑Ho Won
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Affiliations: Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea, Department of Histology and Embryology, Institute of Neuroscience, Wenzhou Medical University, Wenzhou, Zhejiang 325035, P.R. China, Department of Emergency Medicine, School of Medicine, Chuncheon, Gangwon 24341, Republic of Korea, Department of Obstetrics and Gynecology, School of Medicine, Chuncheon, Gangwon 24341, Republic of Korea, Department of Anatomy, College of Veterinary Medicine, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea, Department of Anatomy and Cell Biology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Republic of Korea, Department of Food Science and Nutrition, Hallym University, Chuncheon, Gangwon 24252, Republic of Korea, Department of Biomedical Science, Research Institute of Bioscience and Biotechnology, Hallym University, Chuncheon, Gangwon 24252, Republic of Korea
Published online on: April 13, 2018 https://doi.org/10.3892/mmr.2018.8886
Pages: 8161-8166
Copyright: © Lee et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Insulin‑like growth factor‑I (IGF‑I) is a multifunctional protein present in the central nervous system. A number of previous studies have revealed alterations in IGF‑I and its receptor (IGF‑IR) expression in various regions of the brain. However, there are few reports on age‑dependent alterations in IGF‑I and IGF‑IR expressions in the olfactory bulb, which contains the secondary neurons of the olfactory system. The present study examined the cellular morphology in the olfactory bulb by using cresyl violet (CV) staining at postnatal month (PM) 3 in the young group, PM 6 in the adult group and PM 24 in the aged group in gerbils. In addition, detailed examinations were performed of the protein levels and immunoreactivities of IGF‑I and IGF‑IR in the olfactory bulb in each group. There were no significant changes in the cellular morphology between the three groups. The protein levels and immunoreactivities of the IGF‑I and IGF‑IR were the highest in the young group and they decreased with age. He protein levels and immunoreactivities of the IGF‑I and IGF‑IR were the lowest in the aged group. In brief, our results indicate that IGF‑I and IGF‑IR expressions are strong in young olfactory bulbs and significantly reduced in aged olfactory bulbs. In conclusion, subsequent decreases in IGF‑I and IGF‑IR expression with age may be associated with olfactory decline. Further studies are required to investigate the roles of IFG‑I and IGF‑IR in disorders of the olfactory system.

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