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Article

Effects of triptolide on the expression of MHC II in microglia in kainic acid‑induced epilepsy

  • Authors:
    • Zheng Sun
    • Meng Du
    • Yao Lu
    • Chang‑Qian Zeng
  • View Affiliations / Copyright

    Affiliations: Medical College, Dalian University, Dalian, Liaoning 116622, P.R. China, Neonatal Screening Center, Maternal and Child Health Care Hospital of Dalian, Dalian, Liaoning 116033, P.R. China
  • Pages: 8357-8362
    |
    Published online on: April 18, 2018
       https://doi.org/10.3892/mmr.2018.8891
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Abstract

The purpose of the present study was to determine whether triptolide (T10) had any effect on major histocompatibility complex class II (MHC II) expression in kainic acid (KA)‑activated microglia, and to investigate the underlying molecular mechanism. BV‑2 microglia were pretreated with T10 prior to activation with KA. The expression level of MHC II and class II transactivator (CIITA) mRNA was determined via reverse transcription‑polymerase chain reaction. The expression of MHC II, CIITA and the phosphorylation level of c‑Jun and proto‑oncogene c‑Fos (c‑Fos) was determined by western blotting. The protein expression level of MHC II was determined by immunocytochemistry. It was observed that the mRNA and protein levels of MHC II and CIITA were increased in KA‑activated BV‑2 microglia, and that this increase was almost completely eliminated by T10. AP‑1 is a family of homodimers or heterodimers, composed of Jun family and Fos family proteins. Sequence analysis revealed an AP‑1 DNA binding site in the promoter of CIITA. The phosphorylation of c‑Jun and c‑Fos was increased in KA‑activated microglia, while T10 was able to suppress the phosphorylation of c‑Jun and c‑Fos in KA‑activated microglia. These data suggested that T10 may exert suppressive effects on MHC II expression in KA‑activated microglia, and that the mechanism may involve the regulation of AP‑1 activity.
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Copy and paste a formatted citation
Spandidos Publications style
Sun Z, Du M, Lu Y and Zeng CQ: Effects of triptolide on the expression of MHC II in microglia in kainic acid‑induced epilepsy. Mol Med Rep 17: 8357-8362, 2018.
APA
Sun, Z., Du, M., Lu, Y., & Zeng, C. (2018). Effects of triptolide on the expression of MHC II in microglia in kainic acid‑induced epilepsy. Molecular Medicine Reports, 17, 8357-8362. https://doi.org/10.3892/mmr.2018.8891
MLA
Sun, Z., Du, M., Lu, Y., Zeng, C."Effects of triptolide on the expression of MHC II in microglia in kainic acid‑induced epilepsy". Molecular Medicine Reports 17.6 (2018): 8357-8362.
Chicago
Sun, Z., Du, M., Lu, Y., Zeng, C."Effects of triptolide on the expression of MHC II in microglia in kainic acid‑induced epilepsy". Molecular Medicine Reports 17, no. 6 (2018): 8357-8362. https://doi.org/10.3892/mmr.2018.8891
Copy and paste a formatted citation
x
Spandidos Publications style
Sun Z, Du M, Lu Y and Zeng CQ: Effects of triptolide on the expression of MHC II in microglia in kainic acid‑induced epilepsy. Mol Med Rep 17: 8357-8362, 2018.
APA
Sun, Z., Du, M., Lu, Y., & Zeng, C. (2018). Effects of triptolide on the expression of MHC II in microglia in kainic acid‑induced epilepsy. Molecular Medicine Reports, 17, 8357-8362. https://doi.org/10.3892/mmr.2018.8891
MLA
Sun, Z., Du, M., Lu, Y., Zeng, C."Effects of triptolide on the expression of MHC II in microglia in kainic acid‑induced epilepsy". Molecular Medicine Reports 17.6 (2018): 8357-8362.
Chicago
Sun, Z., Du, M., Lu, Y., Zeng, C."Effects of triptolide on the expression of MHC II in microglia in kainic acid‑induced epilepsy". Molecular Medicine Reports 17, no. 6 (2018): 8357-8362. https://doi.org/10.3892/mmr.2018.8891
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