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Article

Bioinformatics analysis of the molecular mechanisms underlying traumatic spinal cord injury

  • Authors:
    • Shu‑Jie Zhao
    • Wei Zhou
    • Jian Chen
    • Yong‑Jun Luo
    • Guo‑Yong Yin
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China
  • Pages: 8484-8492
    |
    Published online on: April 23, 2018
       https://doi.org/10.3892/mmr.2018.8918
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Abstract

Spinal cord injury (SCI) is a cause of disability. The present study aimed to investigate the molecular mechanisms involved in traumatic SCI. Transcriptome data under accession no. GSE5296, including 96 chips, were downloaded from the Gene Expression Omnibus database. The raw data were normalized and differentially expressed genes (DEGs) were identified. Furthermore, Kyoto Encyclopedia of Genes and Genomes pathway and Gene Ontology enrichment analysis of up‑ and downregulated DEGs was performed. Additionally, a protein‑protein interaction network was constructed and the expression patterns of different genes were determined. Compared with sham samples, there were 374, 707, 1,322, 1,475, 1,724 and 1,342 DEGs identified at 0.5, 4, 24 and 72 h, and 7 and 28 days post‑injury, respectively. At 24 and 72 h, and 7 days following injury, the upregulated DEGs were markedly enriched in ‘inflammatory response’ and ‘immune process’. Downregulated DEGs were predominantly enriched in neuronal function‑associated pathways and ‘steroid biosynthesis’ process. Protein‑protein interaction network analysis demonstrated similar results. Trend charts further demonstrated that the inflammatory and neuronal functions were altered in a temporal and site‑specific manner. The present study provided an insight into the molecular mechanisms underlying traumatic SCI, which may benefit future SCI research and aid in therapy development.
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1 

Ahuja CS, Wilson JR, Nori S, Kotter MRN, Druschel C, Curt A and Fehlings MG: Traumatic spinal cord injury. Nat Rev Dis Primers. 3:170182017. View Article : Google Scholar : PubMed/NCBI

2 

Jia X, Kowalski RG, Sciubba DM and Geocadin RG: Critical care of traumatic spinal cord injury. J Intensive Care Med. 28:12–23. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Siddiqui AM, Khazaei M and Fehlings MG: Translating mechanisms of neuroprotection, regeneration and repair to treatment of spinal cord injury. Prog Brain Res. 218:15–54. 2015. View Article : Google Scholar : PubMed/NCBI

4 

Ahuja CS and Fehlings M: Concise review: Bridging the gap: Novel neuroregenerative and neuroprotective strategies in spinal cord injury. Stem Cells Transl Med. 5:914–924. 2016. View Article : Google Scholar : PubMed/NCBI

5 

Kim YH, Ha KY and Kim SI: Spinal cord injury and related clinical trials. Clin Orthop Surg. 9:1–9. 2017. View Article : Google Scholar : PubMed/NCBI

6 

Bareyre FM and Schwab ME: Inflammation, degeneration and regeneration in the injured spinal cord: Insights from DNA microarrays. Trends Neurosci. 26:555–563. 2003. View Article : Google Scholar : PubMed/NCBI

7 

Ydens E, Palmers I, Hendrix S and Somers V: The next generation of biomarker research in spinal cord injury. Mol Neurobiol. 54:1482–1499. 2017. View Article : Google Scholar : PubMed/NCBI

8 

Wang W, Liu R, Xu Z, Niu X, Mao Z, Meng Q and Cao X: Further insight into molecular mechanism underlying thoracic spinal cord injury using bioinformatics methods. Mol Med Rep. 12:7851–7858. 2015. View Article : Google Scholar : PubMed/NCBI

9 

Chen G, Fang X and Yu M: Regulation of gene expression in rats with spinal cord injury based on microarray data. Mol Med Rep. 12:2465–2472. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Wen T, Hou J, Wang F, Zhang Y, Zhang T and Sun T: Comparative analysis of molecular mechanism of spinal cord injury with time based on bioinformatics data. Spinal Cord. 54:431–438. 2016. View Article : Google Scholar : PubMed/NCBI

11 

Zhu Z, Shen Q, Zhu L and Wei X: Identification of pivotal genes and pathways for spinal cord injury via bioinformatics analysis. Mol Med Rep. 16:3929–3937. 2017. View Article : Google Scholar : PubMed/NCBI

12 

Duan H, Ge W, Zhang A, Xi Y, Chen Z, Luo D, Cheng Y, Fan KS, Horvath S, Sofroniew MV, et al: Transcriptome analyses reveal molecular mechanisms underlying functional recovery after spinal cord injury. Proc Natl Acad Sci USA. 112:13360–13365. 2015. View Article : Google Scholar : PubMed/NCBI

13 

Carvalho BS and Irizarry RA: A framework for oligonucleotide microarray preprocessing. Bioinformatics. 26:2363–2367. 2010. View Article : Google Scholar : PubMed/NCBI

14 

da Huang W, Sherman BT and Lempicki RA: Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nat Protoc. 4:44–57. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Shannon P, Markiel A, Ozier O, Baliga NS, Wang JT, Ramage D, Amin N, Schwikowski B and Ideker T: Cytoscape: A software environment for integrated models of biomolecular interaction networks. Genome Res. 13:2498–2504. 2003. View Article : Google Scholar : PubMed/NCBI

16 

Sun N, Sun W, Li S, Yang J, Yang L, Quan G, Gao X, Wang Z, Cheng X, Li Z, et al: Proteomics analysis of cellular proteins co-immunoprecipitated with nucleoprotein of influenza a virus (H7N9). Int J Mol Sci. 16:25982–25998. 2015. View Article : Google Scholar : PubMed/NCBI

17 

Di Giovanni S, Knoblach SM, Brandoli C, Aden SA, Hoffman EP and Faden AI: Gene profiling in spinal cord injury shows role of cell cycle in neuronal death. Ann Neurol. 53:454–468. 2003. View Article : Google Scholar : PubMed/NCBI

18 

Carmel JB, Galante A, Soteropoulos P, Tolias P, Recce M, Young W and Hart RP: Gene expression profiling of acute spinal cord injury reveals spreading inflammatory signals and neuron loss. Physiol Genomics. 7:201–213. 2001. View Article : Google Scholar : PubMed/NCBI

19 

Kjell J and Olson L: Rat models of spinal cord injury: From pathology to potential therapies. Dis Model Mech. 9:1125–1137. 2016. View Article : Google Scholar : PubMed/NCBI

20 

Saghazadeh A and Rezaei N: The role of timing in the treatment of spinal cord injury. Biomed Pharmacother. 92:128–139. 2017. View Article : Google Scholar : PubMed/NCBI

21 

Kinoshita T, Nakamura T, Umemoto Y, Kojima D, Moriki T, Mitsui T, Goto M, Ishida Y and Tajima F: Increase in interleukin-6 immediately after wheelchair basketball games in persons with spinal cord injury: Preliminary report. Spinal Cord. 51:508–510. 2013. View Article : Google Scholar : PubMed/NCBI

22 

Hu JZ, Huang JH, Xiao ZM, Li JH, Li XM and Lu HB: Tetramethylpyrazine accelerates the function recovery of traumatic spinal cord in rat model by attenuating inflammation. J Neurol Sci. 324:94–99. 2013. View Article : Google Scholar : PubMed/NCBI

23 

David BT, Ratnayake A, Amarante MA, Reddy NP, Dong W, Sampath S, Heary RF and Elkabes S: A toll-like receptor 9 antagonist reduces pain hypersensitivity and the inflammatory response in spinal cord injury. Neurobiol Dis. 54:194–205. 2013. View Article : Google Scholar : PubMed/NCBI

24 

Jin W, Wang J, Zhu T, Yuan B, Ni H, Jiang J, Wang H and Liang W: Anti-inflammatory effects of curcumin in experimental spinal cord injury in rats. Inflamm Res. 63:381–387. 2014. View Article : Google Scholar : PubMed/NCBI

25 

Schroepfer GJ Jr: Sterol biosynthesis. Ann Rev Biochem. 50:585–621. 1981. View Article : Google Scholar : PubMed/NCBI

26 

Ghayee HK and Auchus RJ: Basic concepts and recent developments in human steroid hormone biosynthesis. Rev Endocr Metab Disord. 8:289–300. 2007. View Article : Google Scholar : PubMed/NCBI

27 

Elkabes S and Nicot AB: Sex steroids and neuroprotection in spinal cord injury: A review of preclinical investigations. Exp Neurol. 259:28–37. 2014. View Article : Google Scholar : PubMed/NCBI

28 

Brotfain E, Gruenbaum SE, Boyko M, Kutz R, Zlotnik A and Klein M: Neuroprotection by estrogen and progesterone in traumatic brain injury and spinal cord injury. Curr Neuropharmacol. 14:641–653. 2016. View Article : Google Scholar : PubMed/NCBI

29 

Eftekharpour E, Nagakannan P, Iqbal MA and Chen QM: Mevalonate cascade and small Rho GTPase in spinal cord injury. Curr Mol Pharmacol. 10:141–151. 2017. View Article : Google Scholar : PubMed/NCBI

30 

Flueck JL and Perret C: Vitamin D deficiency in individuals with a spinal cord injury: A literature review. Spinal Cord. 55:428–434. 2017. View Article : Google Scholar : PubMed/NCBI

31 

Bauman WA, Morrison NG and Spungen AM: Vitamin D replacement therapy in persons with spinal cord injury. J Spinal Cord Med. 28:203–207. 2005. View Article : Google Scholar : PubMed/NCBI

32 

Oleson CV, Patel PH and Wuermser LA: Influence of season, ethnicity and chronicity on vitamin D deficiency in traumatic spinal cord injury. J Spinal Cord Med. 33:202–213. 2010. View Article : Google Scholar : PubMed/NCBI

33 

Giatti S, Garcia-Segura LM and Melcangi RC: New steps forward in the neuroactive steroid field. J Steroid Biochem Mol Biol. 153:127–134. 2015. View Article : Google Scholar : PubMed/NCBI

34 

Tuem KB and Atey TM: Neuroactive steroids: Receptor interactions and responses. Front Neurol. 8:4422017. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhao SJ, Zhou W, Chen J, Luo YJ and Yin GY: Bioinformatics analysis of the molecular mechanisms underlying traumatic spinal cord injury. Mol Med Rep 17: 8484-8492, 2018.
APA
Zhao, S., Zhou, W., Chen, J., Luo, Y., & Yin, G. (2018). Bioinformatics analysis of the molecular mechanisms underlying traumatic spinal cord injury. Molecular Medicine Reports, 17, 8484-8492. https://doi.org/10.3892/mmr.2018.8918
MLA
Zhao, S., Zhou, W., Chen, J., Luo, Y., Yin, G."Bioinformatics analysis of the molecular mechanisms underlying traumatic spinal cord injury". Molecular Medicine Reports 17.6 (2018): 8484-8492.
Chicago
Zhao, S., Zhou, W., Chen, J., Luo, Y., Yin, G."Bioinformatics analysis of the molecular mechanisms underlying traumatic spinal cord injury". Molecular Medicine Reports 17, no. 6 (2018): 8484-8492. https://doi.org/10.3892/mmr.2018.8918
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao SJ, Zhou W, Chen J, Luo YJ and Yin GY: Bioinformatics analysis of the molecular mechanisms underlying traumatic spinal cord injury. Mol Med Rep 17: 8484-8492, 2018.
APA
Zhao, S., Zhou, W., Chen, J., Luo, Y., & Yin, G. (2018). Bioinformatics analysis of the molecular mechanisms underlying traumatic spinal cord injury. Molecular Medicine Reports, 17, 8484-8492. https://doi.org/10.3892/mmr.2018.8918
MLA
Zhao, S., Zhou, W., Chen, J., Luo, Y., Yin, G."Bioinformatics analysis of the molecular mechanisms underlying traumatic spinal cord injury". Molecular Medicine Reports 17.6 (2018): 8484-8492.
Chicago
Zhao, S., Zhou, W., Chen, J., Luo, Y., Yin, G."Bioinformatics analysis of the molecular mechanisms underlying traumatic spinal cord injury". Molecular Medicine Reports 17, no. 6 (2018): 8484-8492. https://doi.org/10.3892/mmr.2018.8918
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