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Article Open Access

Cell‑specific and roasting‑dependent regulation of the Keap1/Nrf2 pathway by coffee extracts

  • Authors:
    • Alexandros Priftis
    • Antonia‑Eugenia Angeli‑Terzidou
    • Aristidis S. Veskoukis
    • Demetrios A. Spandidos
    • Dimitrios Kouretas
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry and Biotechnology, University of Thessaly, 41500 Larissa, Greece, Laboratory of Clinical Virology, University of Crete, Medical School, 71409 Heraklion, Crete, Greece
    Copyright: © Priftis et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 8325-8331
    |
    Published online on: April 24, 2018
       https://doi.org/10.3892/mmr.2018.8924
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Abstract

Coffee is a popular beverage that contains various bioactive compounds. However, its molecular mechanism of action is not fully elucidated. In this context, two previously characterized coffee extracts, a lightly roasted and the corresponding green one, were investigated for their effect on nuclear factor erythroid 2‑related factor 2 (Nrf2) target gene expression in myoblasts and endothelial cells using quantitative PCR. The tested concentrations were non‑cytotoxic and led to improved redox cell status, as was evident by increased reduced glutathione (GSH) levels. In both cell lines, the roasted extract upregulated gene expression more readily than its green counterpart leading to increased NAD(P)H quinone dehydrogenase 1 and γ‑glutamyl cysteine ligase catalytic subunit, among others. The green extract had a mixed effect on the endothelial cells, while, as regards the myoblasts it caused the downregulation of some Nrf‑target genes. Therefore, a potential dose‑ and roasting‑dependent mechanism is proposed in the current study, accounting for coffee's antioxidant activity.
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1 

Ray PD, Huang BW and Tsuji Y: Reactive oxygen species (ROS) homeostasis and redox regulation in cellular signaling. Cell Signal. 24:981–990. 2012. View Article : Google Scholar : PubMed/NCBI

2 

Halliwell B: Free radicals and other reactive species in diseaseeLS. John Wiley & Sons, Ltd.; Hoboken, NJ: 2001

3 

Sosa V, Moliné T, Somoza R, Paciucci R, Kondoh H and LLeonart ME: Oxidative stress and cancer: An overview. Ageing Res Rev. 12:376–390. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Rochette L, Zeller M, Cottin Y and Vergely C: Diabetes, oxidative stress and therapeutic strategies. Biochim Biophys Acta. 1840:2709–2729. 2014. View Article : Google Scholar : PubMed/NCBI

5 

Veskoukis AS, Goutianos G, Paschalis V, Margaritelis NV, Tzioura A, Dipla K, Zafeiridis A, Vrabas IS, Kyparos A and Nikolaidis MG: The rat closely mimics oxidative stress and inflammation in humans after exercise but not after exercise combined with vitamin C administration. Eur J Appl Physiol. 116:791–804. 2016. View Article : Google Scholar : PubMed/NCBI

6 

Spanidis Y, Goutzourelas N, Stagos D, Mpesios A, Priftis A, Bar-Or D, Spandidos DA, Tsatsakis AM, Leon G and Kouretas D: Variations in oxidative stress markers in elite basketball players at the beginning and end of a season. Exp Ther Med. 11:147–153. 2016. View Article : Google Scholar : PubMed/NCBI

7 

Deanfield JE, Halcox JP and Rabelink TJ: Endothelial function and dysfunction: Testing and clinical relevance. Circulation. 115:1285–1295. 2007.PubMed/NCBI

8 

Victor VM, Rocha M, Solá E, Bañuls C, Garcia-Malpartida K and Hernández-Mijares A: Oxidative stress, endothelial dysfunction and atherosclerosis. Curr Pharm Des. 15:2988–3002. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Kokura S, Wolf RE, Yoshikawa T, Granger DN and Aw TY: Molecular mechanisms of neutrophil-endothelial cell adhesion induced by redox imbalance. Circ Res. 84:516–524. 1999. View Article : Google Scholar : PubMed/NCBI

10 

Rhee SG: Cell signaling. H2O2, a necessary evil for cell signaling. Science. 312:1882–1883. 2006. View Article : Google Scholar : PubMed/NCBI

11 

Woywodt A, Bahlmann FH, De Groot K, Haller H and Haubitz M: Circulating endothelial cells: Life, death, detachment and repair of the endothelial cell layer. Nephrol Dial Transplant. 17:1728–1730. 2002. View Article : Google Scholar : PubMed/NCBI

12 

Birben E, Sahiner UM, Sackesen C, Erzurum S and Kalayci O: Oxidative stress and antioxidant defense. World Allergy Organ J. 5:9–19. 2012. View Article : Google Scholar : PubMed/NCBI

13 

Pastore A, Federici G, Bertini E and Piemonte F: Analysis of glutathione: Implication in redox and detoxification. Clin Chim Acta. 333:19–39. 2003. View Article : Google Scholar : PubMed/NCBI

14 

Landete JM: Dietary intake of natural antioxidants: Vitamins and polyphenols. Crit Rev Food Sci Nutr. 53:706–721. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Pandey KB and Rizvi SI: Plant polyphenols as dietary antioxidants in human health and disease. Oxid Med Cell Longev. 2:270–278. 2009. View Article : Google Scholar : PubMed/NCBI

16 

Goutzourelas N, Stagos D, Spanidis Y, Liosi M, Apostolou A, Priftis A, Haroutounian S, Spandidos DA, Tsatsakis AM and Kouretas D: Polyphenolic composition of grape stem extracts affects antioxidant activity in endothelial and muscle cells. Mol Med Rep. 12:5846–5856. 2015. View Article : Google Scholar : PubMed/NCBI

17 

Heckman MA, Weil J and de Mejia Gonzalez E: Caffeine (1, 3, 7-trimethylxanthine) in foods: A comprehensive review on consumption, functionality, safety, and regulatory matters. J Food Sci. 75:R77–R87. 2010. View Article : Google Scholar : PubMed/NCBI

18 

Higdon JV and Frei B: Coffee and health: A review of recent human research. Crit Rev Food Sci Nutr. 46:101–123. 2006. View Article : Google Scholar : PubMed/NCBI

19 

Murthy PS and Naidu MM: Recovery of phenolic antioxidants and functional compounds from coffee industry by-productsFood and Bioprocess Technology. 5. Springer; Berlin: pp. 897–903. 2010, View Article : Google Scholar

20 

Henry-Vitrac C, Ibarra A, Roller M, Mérillon JM and Vitrac X: Contribution of chlorogenic acids to the inhibition of human hepatic glucose-6-phosphatase activity in vitro by Svetol, a standardized decaffeinated green coffee extract. J Agric Food Chem. 58:4141–4144. 2010. View Article : Google Scholar : PubMed/NCBI

21 

Park JB: Isolation and quantification of major chlorogenic acids in three major instant coffee brands and their potential effects on H2O2-induced mitochondrial membrane depolarization and apoptosis in PC-12 cells. Food Funct. 4:1632–1638. 2013. View Article : Google Scholar : PubMed/NCBI

22 

Xu J-G, Hu Q-P and Liu Y: Antioxidant and DNA-protective activities of chlorogenic acid isomers. J Agric Food Chem. 60:11625–11630. 2012. View Article : Google Scholar : PubMed/NCBI

23 

Jaiswal R, Matei MF, Golon A, Witt M and Kuhnert N: Understanding the fate of chlorogenic acids in coffee roasting using mass spectrometry based targeted and non-targeted analytical strategies. Food Funct. 3:976–984. 2012. View Article : Google Scholar : PubMed/NCBI

24 

Daglia M, Racchi M, Papetti A, Lanni C, Govoni S and Gazzani G: In vitro and ex vivo antihydroxyl radical activity of green and roasted coffee. J Agric Food Chem. 52:1700–1704. 2004. View Article : Google Scholar : PubMed/NCBI

25 

Priftis A, Papikinos K, Koukoulanaki M, Kerasioti E, Stagos D, Konstantinopoulos K, Spandidos DA, Kermenidou M, Karakitsios S, Sarigiannis D, et al: Development of an assay to assess genotoxicity by particulate matter extract. Mol Med Rep. 15:1738–1746. 2017. View Article : Google Scholar : PubMed/NCBI

26 

Kamiyama M, Moon J-K, Jang HW and Shibamoto T: Role of degradation products of chlorogenic acid in the antioxidant activity of roasted coffee. J Agric Food Chem. 63:1996–2005. 2015. View Article : Google Scholar : PubMed/NCBI

27 

Gawlik-Dziki U, Świeca M, Dziki D, Kowalska I, Pecio Ł, Durak A and Sęczyk Ł: Lipoxygenase inhibitors and antioxidants from green coffee - Mechanism of action in the light of potential bioaccessibility. Food Res Int. 61:48–55. 2014. View Article : Google Scholar

28 

Priftis A, Panagiotou EM, Lakis K, Plika C, Halabalaki M, Ntasi G, Veskoukis AS, Stagos D, Skaltsounis LA and Kouretas D: Roasted and green coffee extracts show antioxidant and cytotoxic activity in myoblast and endothelial cell lines in a cell specific manner. Food Chem Toxicol. 114:119–127. 2018. View Article : Google Scholar : PubMed/NCBI

29 

Aquilano K, Baldelli S and Ciriolo MR: Glutathione: New roles in redox signaling for an old antioxidant. Front Pharmacol. 5:1962014. View Article : Google Scholar : PubMed/NCBI

30 

Sakihama Y, Cohen MF, Grace SC and Yamasaki H: Plant phenolic antioxidant and prooxidant activities: Phenolics-induced oxidative damage mediated by metals in plants. Toxicology. 177:67–80. 2002. View Article : Google Scholar : PubMed/NCBI

31 

Volz N, Boettler U, Winkler S, Teller N, Schwarz C, Bakuradze T, Eisenbrand G, Haupt L, Griffiths LR, Stiebitz H, et al: Effect of coffee combining green coffee bean constituents with typical roasting products on the Nrf2/ARE pathway in vitro and in vivo. J Agric Food Chem. 60:9631–9641. 2012. View Article : Google Scholar : PubMed/NCBI

32 

Higgins LG, Cavin C, Itoh K, Yamamoto M and Hayes JD: Induction of cancer chemopreventive enzymes by coffee is mediated by transcription factor Nrf2. Evidence that the coffee-specific diterpenes cafestol and kahweol confer protection against acrolein. Toxicol Appl Pharmacol. 226:328–337. 2008. View Article : Google Scholar : PubMed/NCBI

33 

Boettler U, Sommerfeld K, Volz N, Pahlke G, Teller N, Somoza V, Lang R, Hofmann T and Marko D: Coffee constituents as modulators of Nrf2 nuclear translocation and ARE (EpRE)-dependent gene expression. J Nutr Biochem. 22:426–440. 2011. View Article : Google Scholar : PubMed/NCBI

34 

Priftis A, Stagos D, Konstantinopoulos K, Tsitsimpikou C, Spandidos DA, Tsatsakis AM, Tzatzarakis MN and Kouretas D: Comparison of antioxidant activity between green and roasted coffee beans using molecular methods. Mol Med Rep. 12:7293–7302. 2015. View Article : Google Scholar : PubMed/NCBI

35 

Priftis A, Goutzourelas N, Halabalaki M, Ntasi G, Stagos D, Amoutzias GD, Skaltsounis LA and Kouretas D: Effect of polyphenols from coffee and grape on gene expression in myoblasts. Mech Ageing Dev. (In press). PubMed/NCBI

36 

Liang N and Kitts DD: Role of chlorogenic acids in controlling oxidative and inflammatory stress conditions. Nutrients. 8:1–20. 2015. View Article : Google Scholar :

37 

Sirota R, Gibson D and Kohen R: The role of the catecholic and the electrophilic moieties of caffeic acid in Nrf2/Keap1 pathway activation in ovarian carcinoma cell lines. Redox Biol. 4:48–59. 2015. View Article : Google Scholar : PubMed/NCBI

38 

Houghton CA, Fassett RG and Coombes JS: Sulforaphane and other nutrigenomic Nrf2 activators: Can the clinician's expectation be matched by the reality? Oxid Med Cell Longev. 2016:78571862016. View Article : Google Scholar : PubMed/NCBI

39 

Alvarez S and Boveris A: Antioxidant adaptive response of human mononuclear cells to UV-B: Effect of lipoic acid. J Photochem Photobiol B. 55:113–119. 2000. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Priftis A, Angeli‑Terzidou AE, Veskoukis AS, Spandidos DA and Kouretas D: Cell‑specific and roasting‑dependent regulation of the Keap1/Nrf2 pathway by coffee extracts. Mol Med Rep 17: 8325-8331, 2018.
APA
Priftis, A., Angeli‑Terzidou, A., Veskoukis, A.S., Spandidos, D.A., & Kouretas, D. (2018). Cell‑specific and roasting‑dependent regulation of the Keap1/Nrf2 pathway by coffee extracts. Molecular Medicine Reports, 17, 8325-8331. https://doi.org/10.3892/mmr.2018.8924
MLA
Priftis, A., Angeli‑Terzidou, A., Veskoukis, A. S., Spandidos, D. A., Kouretas, D."Cell‑specific and roasting‑dependent regulation of the Keap1/Nrf2 pathway by coffee extracts". Molecular Medicine Reports 17.6 (2018): 8325-8331.
Chicago
Priftis, A., Angeli‑Terzidou, A., Veskoukis, A. S., Spandidos, D. A., Kouretas, D."Cell‑specific and roasting‑dependent regulation of the Keap1/Nrf2 pathway by coffee extracts". Molecular Medicine Reports 17, no. 6 (2018): 8325-8331. https://doi.org/10.3892/mmr.2018.8924
Copy and paste a formatted citation
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Spandidos Publications style
Priftis A, Angeli‑Terzidou AE, Veskoukis AS, Spandidos DA and Kouretas D: Cell‑specific and roasting‑dependent regulation of the Keap1/Nrf2 pathway by coffee extracts. Mol Med Rep 17: 8325-8331, 2018.
APA
Priftis, A., Angeli‑Terzidou, A., Veskoukis, A.S., Spandidos, D.A., & Kouretas, D. (2018). Cell‑specific and roasting‑dependent regulation of the Keap1/Nrf2 pathway by coffee extracts. Molecular Medicine Reports, 17, 8325-8331. https://doi.org/10.3892/mmr.2018.8924
MLA
Priftis, A., Angeli‑Terzidou, A., Veskoukis, A. S., Spandidos, D. A., Kouretas, D."Cell‑specific and roasting‑dependent regulation of the Keap1/Nrf2 pathway by coffee extracts". Molecular Medicine Reports 17.6 (2018): 8325-8331.
Chicago
Priftis, A., Angeli‑Terzidou, A., Veskoukis, A. S., Spandidos, D. A., Kouretas, D."Cell‑specific and roasting‑dependent regulation of the Keap1/Nrf2 pathway by coffee extracts". Molecular Medicine Reports 17, no. 6 (2018): 8325-8331. https://doi.org/10.3892/mmr.2018.8924
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