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Article Open Access

Effect of combined chondroitinase ABC and hyperbaric oxygen therapy in a rat model of spinal cord injury

  • Authors:
    • Xiaoyang Liu
    • Jiefeng Wang
    • Guangkuo Li
    • Honglin Lv
  • View Affiliations / Copyright

    Affiliations: Department of Spine Surgery, Yantai Yuhuangding Hospital, Yantai, Shandong 264000, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 25-30
    |
    Published online on: April 26, 2018
       https://doi.org/10.3892/mmr.2018.8933
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Abstract

The present study aimed to investigate the effect of combined hyperbaric oxygen (HBO) and chondroitinase ABC (ChABC) enzyme therapy in a rat model of spinal cord injury (SCI) and to explore the underlying mechanisms. A total of 48 healthy male Wistar rats were randomly divided into six groups: Sham, SCI, vehicle, HBO, ChABC enzyme and HBO + ChABC. Excluding the sham group, SCI was established in rats by a clip compression injury and rats subsequently received HBO treatment for 2 weeks with or without an intraspinal injection of 0.1 U/µl ChABC. Neuromotor functions were examined using the Basso‑Beattie‑Bresnahan locomotor rating scale and the inclined plane assessment at baseline and for 4 weeks following SCI establishment. Superoxide dismutase (SOD) and malondialdehyde (MDA) levels were also measured, in addition to the expression of glycogen synthase kinase‑3β (GSK3β) and aquaporin 4 (AQP4). Results revealed that combined HBO and ChABC treatment significantly improved neuromotor function compared with the HBO or ChABC treatments alone. HBO and/or ChABC treatment significantly increased SOD and decreased MDA levels, as well as GSK3β expression, compared with the sham and SCI rats. The combined HBO and ChABC treatment significantly inhibited SCI‑induced AQP4 expression, but ChABC alone did not. Functional recovery in the HBO + ChABC group was significantly increased compared with the HBO or ChABC groups. These results indicate that combined HBO and ChABC treatment is more effective in treating SCI than either therapy alone.
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Copy and paste a formatted citation
Spandidos Publications style
Liu X, Wang J, Li G and Lv H: Effect of combined chondroitinase ABC and hyperbaric oxygen therapy in a rat model of spinal cord injury. Mol Med Rep 18: 25-30, 2018.
APA
Liu, X., Wang, J., Li, G., & Lv, H. (2018). Effect of combined chondroitinase ABC and hyperbaric oxygen therapy in a rat model of spinal cord injury. Molecular Medicine Reports, 18, 25-30. https://doi.org/10.3892/mmr.2018.8933
MLA
Liu, X., Wang, J., Li, G., Lv, H."Effect of combined chondroitinase ABC and hyperbaric oxygen therapy in a rat model of spinal cord injury". Molecular Medicine Reports 18.1 (2018): 25-30.
Chicago
Liu, X., Wang, J., Li, G., Lv, H."Effect of combined chondroitinase ABC and hyperbaric oxygen therapy in a rat model of spinal cord injury". Molecular Medicine Reports 18, no. 1 (2018): 25-30. https://doi.org/10.3892/mmr.2018.8933
Copy and paste a formatted citation
x
Spandidos Publications style
Liu X, Wang J, Li G and Lv H: Effect of combined chondroitinase ABC and hyperbaric oxygen therapy in a rat model of spinal cord injury. Mol Med Rep 18: 25-30, 2018.
APA
Liu, X., Wang, J., Li, G., & Lv, H. (2018). Effect of combined chondroitinase ABC and hyperbaric oxygen therapy in a rat model of spinal cord injury. Molecular Medicine Reports, 18, 25-30. https://doi.org/10.3892/mmr.2018.8933
MLA
Liu, X., Wang, J., Li, G., Lv, H."Effect of combined chondroitinase ABC and hyperbaric oxygen therapy in a rat model of spinal cord injury". Molecular Medicine Reports 18.1 (2018): 25-30.
Chicago
Liu, X., Wang, J., Li, G., Lv, H."Effect of combined chondroitinase ABC and hyperbaric oxygen therapy in a rat model of spinal cord injury". Molecular Medicine Reports 18, no. 1 (2018): 25-30. https://doi.org/10.3892/mmr.2018.8933
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