Expression analysis of ST3GAL4 transcripts in cervical cancer cells

Roa‑de La Cruz,Lorena; Martínez‑Morales,Patricia; Morán‑Cruz,Irene; Milflores‑Flores,Lorena; Rosas‑Murrieta,Nora; González‑Ramírez,César; Ortiz‑Mateos,Claudia; Monterrosas‑Santamaría,Ricardo; González‑Frías,Celestina; Rodea‑Ávila,Carlos; Apresa‑García,Teresa; Aguilar‑Lemarroy,Adriana; Jave‑Suarez,Luis; Santos‑López,Gerardo; Reyes‑Leyva,Julio; Vallejo‑Ruiz,Verónica

doi:10.3892/mmr.2018.8938

July-2018 Volume 18 Issue 1

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July-2018 Volume 18 Issue 1

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Article

Expression analysis of ST3GAL4 transcripts in cervical cancer cells

Authors:
- Lorena Roa‑de La Cruz
- Patricia Martínez‑Morales
- Irene Morán‑Cruz
- Lorena Milflores‑Flores
- Nora Rosas‑Murrieta
- César González‑Ramírez
- Claudia Ortiz‑Mateos
- Ricardo Monterrosas‑Santamaría
- Celestina González‑Frías
- Carlos Rodea‑Ávila
- Teresa Apresa‑García
- Adriana Aguilar‑Lemarroy
- Luis Jave‑Suarez
- Gerardo Santos‑López
- Julio Reyes‑Leyva
- Verónica Vallejo‑Ruiz
View Affiliations / Copyright

Affiliations: Instituto Mexicano del Seguro Social, Centro de Investigación Biomédica de Oriente, Laboratorio de Biología Molecular, C.P. Metepec, Puebla 74360, Mexico, CONACYT‑ Centro de Investigación Biomédica de Oriente (CIBIOR), Metepec, Puebla 74360, Mexico, Escuela de Biología, Benemérita Universidad Autónoma de Puebla, Puebla, Puebla 72570, Mexico, Laboratorio de Bioquímica y Biología Molecular, Instituto de Ciencias Benemérita Universidad Autónoma de Puebla, Puebla, Puebla 72570, Mexico, Instituto Mexicano del Seguro Social, Hospital General de Zona No. 15, Tehuacán, Puebla 75710, Mexico, Instituto Mexicano del Seguro Social, Hospital General Regional No. 36, Puebla, Puebla 72090, Mexico, Instituto Mexicano del Seguro Social, Centro Médico Nacional sXXI, Mexico City 06720, Mexico, Instituto Mexicano del Seguro Social, Centro de Investigación Biomédica de Occidente, Guadalajara, Jalisco 44340, Mexico
Pages: 617-621
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Published online on: April 27, 2018

https://doi.org/10.3892/mmr.2018.8938
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Abstract

ST3GAL4 gene expression is altered in different cancer types, including cervical cancer. Several mRNA transcripts have been reported for this gene; however, their expression levels in cervical cancer have not been analyzed. ST3GAL4 encodes for β‑galactosidase α‑2,3‑sialyltransferase 4, involved in the biosynthesis of the tumour antigens sLe(x) and sulfo‑sLe(x). The present study evaluated the presence of three mRNA variants (V1, V2 and V3) in cervical cancer cell lines, detecting the three variants. Additionally, the expression level of the V1 transcript of the ST3GAL4 gene was determined by reverse transcription‑quantitative polymerase chain reaction in cervical cell lines and in normal, premalignant and cervical cancer tissue. The V1 transcript of the ST3GAL4 demonstrated significant decreased expression in premalignant and malignant cervical tissues. The results suggested that deregulation of this gene could occur prior to the presence of cancer and demonstrated the importance of evaluating the expression level of V1, and its association with disease progression.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Expression analysis of ST3GAL4 transcripts in cervical cancer cells

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