Placental protein 14 as a potential biomarker for diagnosis of preterm premature rupture of membranes

Wang,Yanyun; Luo,Haibo; Che,Guanglu; Li,Yanqin; Gao,Jun; Yang,Qiongli; Zhou,Bin; Gao,Linbo; Wang,Tao; Liang,Yujie; Zhang,Lin

doi:10.3892/mmr.2018.8967

July-2018 Volume 18 Issue 1

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July-2018 Volume 18 Issue 1

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Article Open Access

Placental protein 14 as a potential biomarker for diagnosis of preterm premature rupture of membranes

Authors:
- Yanyun Wang
- Haibo Luo
- Guanglu Che
- Yanqin Li
- Jun Gao
- Qiongli Yang
- Bin Zhou
- Linbo Gao
- Tao Wang
- Yujie Liang
- Lin Zhang
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Affiliations: Laboratory of Molecular Translational Medicine, Center for Translational Medicine, Key Laboratory of Birth Defects and Related Diseases of Women and Children, Ministry of Education, Department of Obstetrics and Gynecology, West China Second University Hospital, Chengdu, Sichuan 610041, P.R. China, Institute of Forensic Medicine, West China School of Basic Sciences and Forensic Medicine, Sichuan University, Chengdu, Sichuan 610041, P.R. China, Department of Perinatal Healthcare, Shuangliu District Maternal and Child Health Hospital, Chengdu, Sichuan 610200, P.R. China

Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 113-122
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Published online on: May 3, 2018

https://doi.org/10.3892/mmr.2018.8967
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Abstract

Premature rupture of membranes (PROM) is a common pregnancy complication that frequently results in maternal and perinatal morbidity. The present methods for diagnosing PROM do not satisfy clinical requirements. The present study aimed to examine the proteome profile of amniotic fluid (AF) and maternal plasma, screen unique proteins in AF, and evaluate their diagnostic value for diagnosing PROM. The proteome profiles of AF and maternal plasma were examined via liquid chromatography coupled with tandem mass spectrometry‑based proteomic techniques. The protein expression levels of diagnostic candidates in AF, maternal plasma and vaginal fluid were determined by ELISA analysis and Magnetic Luminex® screening assays. The diagnostic value of potential biomarkers was evaluated using receiver operating characteristic curves. A lateral flow assay was developed based on colloidal gold immunochromatography technology. The present study identified 540 unique proteins in AF, 12 of which were chosen for further detection. The present results demonstrated that expression levels of pulmonary surfactant‑associated protein B, BPI fold‑containing family A member 1, zymogen granule protein 16 homolog B, EGF‑containing fibulin‑like extracellular matrix protein 1, keratin, type II cytoskeletal 4, keratin, type I cytoskeletal 19, placental protein 14 (PP14), insulin‑like growth factor‑binding protein 2, mesothelin and serpin family B member 3 were significantly higher in AF compared with in maternal plasma (P<0.01). Furthermore, PP14 was observed to have excellent diagnostic accuracy for preterm PROM (PPROM), with a respective sensitivity and specificity of 100 and 87.5% when the cutoff value was 0.008 µg/ml. The PP14‑based lateral flow assay demonstrated a visual detection threshold of 0.008 µg/ml. The results from the present study suggested that PP14 may be a novel potential biomarker for PPROM, and may be developed into a lateral flow assay for bedside application to rapidly diagnose PPROM.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Placental protein 14 as a potential biomarker for diagnosis of preterm premature rupture of membranes

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