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Toll‑Like receptor 4 promotes the phosphorylation of CRMP2 via the activation of Rho‑kinase in MCAO rats

  • Authors:
    • Xue‑Bo Li
    • Ming‑Xia Ding
    • Chun‑Li Ding
    • Liang‑Liang Li
    • Jinzhou Feng
    • Xiao‑Jun Yu
  • View Affiliations / Copyright

    Affiliations: Department of Forensic Medicine, Shantou University Medical College, Shantou, Guangdong 515041, P.R. China, Department of Obstetrics and Gynecology, The Second Hospital of Shandong University, Jinan, Shandong 250014, P.R. China, Key Laboratory of Evidence Identification in Universities of Shandong, Shandong University of Political Science and Law, Chongqing 400016, P.R. China, Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 342-348
    |
    Published online on: May 3, 2018
       https://doi.org/10.3892/mmr.2018.8968
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Abstract

The mechanism associated with Toll‑like receptor 4 (TLR4) in neurological injury remains unclear. The aim of the present study was to investigate the pathology of TLR4 in middle cerebral artery occlusion (MCAO)/reperfusion rat models via the regulation of collapsin response mediator protein 2 (CRMP2) phosphorylation. The modified neurological severity score (mNSS) was applied to assess neurological recovery. Immunofluorescence and western blotting were used to detect the protein expressions of TLR4, Rho‑associated protein kinase 2 (ROCK‑II) and CRMP2 following the intracerebroventricular administration of TLR4‑specific agonist, lipopolysaccharide (LPS) and TLR4‑neutralizing antibody, the ROCK‑II specific inhibitor Y‑27632 or LPS+Y‑27632 30 min prior to MCAO. The expression levels of TLR4 and the phosphorylation of CRMP2 significantly increased in response to LPS‑mediated induction and/or MCAO; however, they were reversed by treatment with LPS+TLR4‑neutralizing antibody. Y‑27632 decreased the expression of ROCK‑II and phosphorylated (p)‑CRMP2, and suppressed the increased ROCK‑II and p‑CRMP2 induced by LPS; however, no effect on the levels of TLR4 expression was observed. The neurological function as measured by mNSS score was reduced in the LPS group when compared with the MCAO group, whereas the LPS+Y‑27632 group reversed the reduced neurological function at 7 and 14 days post‑MCAO. The results of the present study suggested that TLR4 may promote the phosphorylation of CRMP2 via the activation of ROCK‑II in MCAO rats, which further characterizes the pathological mechanism of TLR4 in stroke, and that modulation of TLR4 could be a potential target to limit secondary post‑stroke brain damage.
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Copy and paste a formatted citation
Spandidos Publications style
Li XB, Ding MX, Ding CL, Li LL, Feng J and Yu XJ: Toll‑Like receptor 4 promotes the phosphorylation of CRMP2 via the activation of Rho‑kinase in MCAO rats. Mol Med Rep 18: 342-348, 2018.
APA
Li, X., Ding, M., Ding, C., Li, L., Feng, J., & Yu, X. (2018). Toll‑Like receptor 4 promotes the phosphorylation of CRMP2 via the activation of Rho‑kinase in MCAO rats. Molecular Medicine Reports, 18, 342-348. https://doi.org/10.3892/mmr.2018.8968
MLA
Li, X., Ding, M., Ding, C., Li, L., Feng, J., Yu, X."Toll‑Like receptor 4 promotes the phosphorylation of CRMP2 via the activation of Rho‑kinase in MCAO rats". Molecular Medicine Reports 18.1 (2018): 342-348.
Chicago
Li, X., Ding, M., Ding, C., Li, L., Feng, J., Yu, X."Toll‑Like receptor 4 promotes the phosphorylation of CRMP2 via the activation of Rho‑kinase in MCAO rats". Molecular Medicine Reports 18, no. 1 (2018): 342-348. https://doi.org/10.3892/mmr.2018.8968
Copy and paste a formatted citation
x
Spandidos Publications style
Li XB, Ding MX, Ding CL, Li LL, Feng J and Yu XJ: Toll‑Like receptor 4 promotes the phosphorylation of CRMP2 via the activation of Rho‑kinase in MCAO rats. Mol Med Rep 18: 342-348, 2018.
APA
Li, X., Ding, M., Ding, C., Li, L., Feng, J., & Yu, X. (2018). Toll‑Like receptor 4 promotes the phosphorylation of CRMP2 via the activation of Rho‑kinase in MCAO rats. Molecular Medicine Reports, 18, 342-348. https://doi.org/10.3892/mmr.2018.8968
MLA
Li, X., Ding, M., Ding, C., Li, L., Feng, J., Yu, X."Toll‑Like receptor 4 promotes the phosphorylation of CRMP2 via the activation of Rho‑kinase in MCAO rats". Molecular Medicine Reports 18.1 (2018): 342-348.
Chicago
Li, X., Ding, M., Ding, C., Li, L., Feng, J., Yu, X."Toll‑Like receptor 4 promotes the phosphorylation of CRMP2 via the activation of Rho‑kinase in MCAO rats". Molecular Medicine Reports 18, no. 1 (2018): 342-348. https://doi.org/10.3892/mmr.2018.8968
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