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Article Open Access

MEF2‑activated long non‑coding RNA PCGEM1 promotes cell proliferation in hormone‑refractory prostate cancer through downregulation of miR‑148a

  • Authors:
    • Shibao Zhang
    • Zongwu Li
    • Longyang Zhang
    • Zhonghua Xu
  • View Affiliations / Copyright

    Affiliations: Department of Urology, Ji'nan Central Hospital Affiliated to Shandong University, Ji'nan, Shandong 250013, P.R. China, Department of Urology, Qilu Hospital of Shandong University, Ji'nan, Shandong 250012, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 202-208
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    Published online on: May 4, 2018
       https://doi.org/10.3892/mmr.2018.8977
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Abstract

Prostate cancer gene expression marker 1 (PCGEM1) is a prostate‑specific gene overexpressed in prostate cancer cells that promotes cell proliferation. To study the molecular mechanism of PCGEM1 function in hormone‑refractory prostate cancer, the interaction between myocyte enhancer factor 2 (MEF2) and PCGEM1 was assessed by a luciferase reporter assay and chromatin immunoprecipitation (ChIP) assay. In addition, the underlying mechanism of PCGEM1 regulating expression of microRNA (miR)‑148a in PC3 prostate cancer cells was evaluated. Relative expression levels were measured by reverse transcription‑quantitative polymerase chain reaction, and early apoptosis was measured by flow cytometry. PCGEM1 was demonstrated to be overexpressed in prostate cancer tissues compared with noncancerous tissues. Expression levels of PCGEM1 in PC3 cancer cells were demonstrated to be regulated by MEF2, as PCGME1 mRNA was increased by MEF2 overexpression but decreased by MEF2 silencing. MEF2 was also demonstrated to enhance the activity of PCGEM1 promoter and thus promote PCGEM1 transcription. In addition, downregulation of PCGEM1 expression in PC3 cells increased expression of miR‑148a. By contrast, overexpression of PCGEM1 decreased miR‑148a expression. Finally, PCGME1 silencing by small interfering RNA significantly induced early cell apoptosis but this effect was reduced by a miR‑148a inhibitor. In conclusion, the present study demonstrated a positive regulatory association between MEF2 and PCGEM1, and a reciprocal negative regulatory association between PCGEM1 and miR‑148a that controls cell apoptosis. The present study, therefore, provides new insights into the mechanism of PCGEM1 function in prostate cancer development.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang S, Li Z, Zhang L and Xu Z: MEF2‑activated long non‑coding RNA PCGEM1 promotes cell proliferation in hormone‑refractory prostate cancer through downregulation of miR‑148a. Mol Med Rep 18: 202-208, 2018.
APA
Zhang, S., Li, Z., Zhang, L., & Xu, Z. (2018). MEF2‑activated long non‑coding RNA PCGEM1 promotes cell proliferation in hormone‑refractory prostate cancer through downregulation of miR‑148a. Molecular Medicine Reports, 18, 202-208. https://doi.org/10.3892/mmr.2018.8977
MLA
Zhang, S., Li, Z., Zhang, L., Xu, Z."MEF2‑activated long non‑coding RNA PCGEM1 promotes cell proliferation in hormone‑refractory prostate cancer through downregulation of miR‑148a". Molecular Medicine Reports 18.1 (2018): 202-208.
Chicago
Zhang, S., Li, Z., Zhang, L., Xu, Z."MEF2‑activated long non‑coding RNA PCGEM1 promotes cell proliferation in hormone‑refractory prostate cancer through downregulation of miR‑148a". Molecular Medicine Reports 18, no. 1 (2018): 202-208. https://doi.org/10.3892/mmr.2018.8977
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang S, Li Z, Zhang L and Xu Z: MEF2‑activated long non‑coding RNA PCGEM1 promotes cell proliferation in hormone‑refractory prostate cancer through downregulation of miR‑148a. Mol Med Rep 18: 202-208, 2018.
APA
Zhang, S., Li, Z., Zhang, L., & Xu, Z. (2018). MEF2‑activated long non‑coding RNA PCGEM1 promotes cell proliferation in hormone‑refractory prostate cancer through downregulation of miR‑148a. Molecular Medicine Reports, 18, 202-208. https://doi.org/10.3892/mmr.2018.8977
MLA
Zhang, S., Li, Z., Zhang, L., Xu, Z."MEF2‑activated long non‑coding RNA PCGEM1 promotes cell proliferation in hormone‑refractory prostate cancer through downregulation of miR‑148a". Molecular Medicine Reports 18.1 (2018): 202-208.
Chicago
Zhang, S., Li, Z., Zhang, L., Xu, Z."MEF2‑activated long non‑coding RNA PCGEM1 promotes cell proliferation in hormone‑refractory prostate cancer through downregulation of miR‑148a". Molecular Medicine Reports 18, no. 1 (2018): 202-208. https://doi.org/10.3892/mmr.2018.8977
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