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Genome‑wide DNA methylation profiling in a rat model with vascular dementia

  • Authors:
    • Jong‑Min Park
    • Yoon Ju Kim
    • Min Kyung Song
    • Jae‑Min Lee
    • Youn‑Jung Kim
  • View Affiliations / Copyright

    Affiliations: Department of Nursing, Graduate School, Kyung Hee University, Seoul 02447, Republic of Korea, Department of Physiology, College of Medicine, Kyung Hee University, Seoul 02447, Republic of Korea, College of Nursing Science, Kyung Hee University, Seoul 02447, Republic of Korea
    Copyright: © Park et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 123-130
    |
    Published online on: May 8, 2018
       https://doi.org/10.3892/mmr.2018.8990
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Abstract

Vascular dementia (VaD), the second most prevalent type of dementia, is caused by reduced blood supply to the brain that results in cognitive impairment. Despite the efforts of numerous studies, the pathological mechanisms behind VaD remain unclear. The aim of the present study was to identify candidate genes that undergo changes in hippocampal DNA methylation owing to VaD. A genome‑wide DNA methylation analysis was performed, using methylated DNA‑binding domain sequencing. VaD model rats with cognitive impairment induced by bilateral common carotid artery occlusion were confirmed using the radial arm maze test. A total of 1,180 differentially methylated genes (DMGs) were identified, and functional annotation analysis revealed the DMGs to be enriched in 10 Gene Ontology biological processes. Network analysis using the STRING database indicated that seven genes were closely connected. Rats in the VaD model group demonstrated relative hypomethylation in the promoter region and increased mRNA expression of the hippocampal genes vascular endothelial growth factor (VEGFA) and kinase insert domain receptor, but only differences in VEGFA mRNA expression levels were determined to be statistically significant. In conclusion, these preliminary data from the functional annotation of hippocampal DMGs in the promoter region highlighted candidate genes for VaD that may contribute to the elucidation of its pathophysiology.
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Copy and paste a formatted citation
Spandidos Publications style
Park JM, Kim YJ, Song MK, Lee JM and Kim YJ: Genome‑wide DNA methylation profiling in a rat model with vascular dementia. Mol Med Rep 18: 123-130, 2018.
APA
Park, J., Kim, Y.J., Song, M.K., Lee, J., & Kim, Y. (2018). Genome‑wide DNA methylation profiling in a rat model with vascular dementia. Molecular Medicine Reports, 18, 123-130. https://doi.org/10.3892/mmr.2018.8990
MLA
Park, J., Kim, Y. J., Song, M. K., Lee, J., Kim, Y."Genome‑wide DNA methylation profiling in a rat model with vascular dementia". Molecular Medicine Reports 18.1 (2018): 123-130.
Chicago
Park, J., Kim, Y. J., Song, M. K., Lee, J., Kim, Y."Genome‑wide DNA methylation profiling in a rat model with vascular dementia". Molecular Medicine Reports 18, no. 1 (2018): 123-130. https://doi.org/10.3892/mmr.2018.8990
Copy and paste a formatted citation
x
Spandidos Publications style
Park JM, Kim YJ, Song MK, Lee JM and Kim YJ: Genome‑wide DNA methylation profiling in a rat model with vascular dementia. Mol Med Rep 18: 123-130, 2018.
APA
Park, J., Kim, Y.J., Song, M.K., Lee, J., & Kim, Y. (2018). Genome‑wide DNA methylation profiling in a rat model with vascular dementia. Molecular Medicine Reports, 18, 123-130. https://doi.org/10.3892/mmr.2018.8990
MLA
Park, J., Kim, Y. J., Song, M. K., Lee, J., Kim, Y."Genome‑wide DNA methylation profiling in a rat model with vascular dementia". Molecular Medicine Reports 18.1 (2018): 123-130.
Chicago
Park, J., Kim, Y. J., Song, M. K., Lee, J., Kim, Y."Genome‑wide DNA methylation profiling in a rat model with vascular dementia". Molecular Medicine Reports 18, no. 1 (2018): 123-130. https://doi.org/10.3892/mmr.2018.8990
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