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RNA‑sequencing analysis of aberrantly expressed long non‑coding RNAs and mRNAs in a mouse model of ventilator‑induced lung injury

  • Authors:
    • Bo Xu
    • Yizhou Wang
    • Xiujuan Li
    • Yanfei Mao
    • Xiaoming Deng
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology and Intensive Care, Changhai Hospital, Second Military Medical University, Shanghai 200433, P.R. China, Department of Hepatic Surgery IV, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, P.R. China, Department of Anesthesiology and SICU, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200092, P.R. China
    Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 882-892
    |
    Published online on: May 17, 2018
       https://doi.org/10.3892/mmr.2018.9034
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Abstract

Long non-coding RNAs (lncRNAs) are closely associated with the regulation of various biological processes and are involved in the pathogenesis of numerous diseases. However, to the best of our knowledge, the role of lncRNAs in ventilator‑induced lung injury (VILI) has yet to be evaluated. In the present study, high‑throughput sequencing was applied to investigate differentially expressed lncRNAs and mRNAs (fold change >2; false discovery rate <0.05). Bioinformatics analysis was employed to predict the functions of differentially expressed lncRNAs. A total of 104 lncRNAs (74 upregulated and 30 downregulated) and 809 mRNAs (521 upregulated and 288 downregulated) were differentially expressed in lung tissues from the VILI group. Gene ontology analysis demonstrated that the differentially expressed lncRNAs and mRNAs were mainly associated with biological functions, including apoptosis, angiogenesis, neutrophil chemotaxis and skeletal muscle cell differentiation. The top four enriched pathways were the tumor necrosis factor (TNF) signaling pathway, P53 signaling pathway, neuroactive ligand‑receptor interaction and the forkhead box O signaling pathway. Several lncRNAs were predicted to serve a vital role in VILI. Subsequently, three lncRNAs [mitogen‑activated protein kinase kinase 3, opposite strand (Map2k3os), dynamin 3, opposite strand and abhydrolase domain containing 11, opposite strand] and three mRNAs (growth arrest and DNA damage‑inducible α, claudin 4 and thromboxane A2 receptor) were measured by reverse transcription‑quantitative polymerase chain reaction, in order to confirm the veracity of RNA‑sequencing analysis. In addition, Map2k3os small interfering RNA transfection inhibited the expression of stretch‑induced cytokines [TNF‑α, interleukin (IL)‑1β and IL‑6] in MLE12 cells. In conclusion, the results of the present study provided a profile of differentially expressed lncRNAs in VILI. Several important lncRNAs may be involved in the pathological process of VILI, which may be useful to guide further investigation into the pathogenesis for this disease.
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Copy and paste a formatted citation
Spandidos Publications style
Xu B, Wang Y, Li X, Mao Y and Deng X: RNA‑sequencing analysis of aberrantly expressed long non‑coding RNAs and mRNAs in a mouse model of ventilator‑induced lung injury. Mol Med Rep 18: 882-892, 2018.
APA
Xu, B., Wang, Y., Li, X., Mao, Y., & Deng, X. (2018). RNA‑sequencing analysis of aberrantly expressed long non‑coding RNAs and mRNAs in a mouse model of ventilator‑induced lung injury. Molecular Medicine Reports, 18, 882-892. https://doi.org/10.3892/mmr.2018.9034
MLA
Xu, B., Wang, Y., Li, X., Mao, Y., Deng, X."RNA‑sequencing analysis of aberrantly expressed long non‑coding RNAs and mRNAs in a mouse model of ventilator‑induced lung injury". Molecular Medicine Reports 18.1 (2018): 882-892.
Chicago
Xu, B., Wang, Y., Li, X., Mao, Y., Deng, X."RNA‑sequencing analysis of aberrantly expressed long non‑coding RNAs and mRNAs in a mouse model of ventilator‑induced lung injury". Molecular Medicine Reports 18, no. 1 (2018): 882-892. https://doi.org/10.3892/mmr.2018.9034
Copy and paste a formatted citation
x
Spandidos Publications style
Xu B, Wang Y, Li X, Mao Y and Deng X: RNA‑sequencing analysis of aberrantly expressed long non‑coding RNAs and mRNAs in a mouse model of ventilator‑induced lung injury. Mol Med Rep 18: 882-892, 2018.
APA
Xu, B., Wang, Y., Li, X., Mao, Y., & Deng, X. (2018). RNA‑sequencing analysis of aberrantly expressed long non‑coding RNAs and mRNAs in a mouse model of ventilator‑induced lung injury. Molecular Medicine Reports, 18, 882-892. https://doi.org/10.3892/mmr.2018.9034
MLA
Xu, B., Wang, Y., Li, X., Mao, Y., Deng, X."RNA‑sequencing analysis of aberrantly expressed long non‑coding RNAs and mRNAs in a mouse model of ventilator‑induced lung injury". Molecular Medicine Reports 18.1 (2018): 882-892.
Chicago
Xu, B., Wang, Y., Li, X., Mao, Y., Deng, X."RNA‑sequencing analysis of aberrantly expressed long non‑coding RNAs and mRNAs in a mouse model of ventilator‑induced lung injury". Molecular Medicine Reports 18, no. 1 (2018): 882-892. https://doi.org/10.3892/mmr.2018.9034
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