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Article

G protein‑coupled estrogen receptor/miR‑148a/human leukocyte antigen‑G signaling pathway mediates cell apoptosis of ovarian endometriosis

  • Authors:
    • Shun Zhi He
    • Jing Li
    • Hong Chu Bao
    • Mei Mei Wang
    • Xin Rong Wang
    • Xin Huang
    • Feng Hua Li
    • Wei Zhang
    • An Li Xu
    • Hao Cui Fang
    • Yang Xing Sheng
  • View Affiliations / Copyright

    Affiliations: Department of Gynecology and Obstetrics, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China, Electrocardiogram Room, Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong 264000, P.R. China, Reproductive Medicine Center, Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong 264000, P.R. China, Department of Gynecology, Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong 264000, P.R. China
  • Pages: 1141-1148
    |
    Published online on: May 21, 2018
       https://doi.org/10.3892/mmr.2018.9039
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Abstract

The focus of the current study was a G protein‑coupled estrogen receptor (GPER)/microRNA (miR)‑148a/human leukocyte antigen‑G (HLA‑G) signaling pathway in ovarian endometriosis. Reverse transcription‑quantitative polymerase chain reaction was performed to analyze the changes in miR‑148a expression. A MTT assay, flow cytometry and caspase‑3/9 activity assays were performed to analyze cell proliferation, apoptosis and caspase‑3/9 activity levels, respectively. Protein expression was measured using western blot analysis. In tissue samples from healthy controls, and patients with endometriosis and endometriosis‑associated ovarian cancer, the expression of miR‑148a was lower in in endometriosis and EAOC samples compared with healthy controls. Overexpression of miR‑148a using miR mimics significantly decreased proliferation, promoted apoptosis, increased the Bcl‑2 associated X apoptosis regulator (Bax)/Bcl‑2 apoptosis regulator (Bcl‑2) ratio and caspase3/9 activity, and suppressed HLA‑G protein expression in Hs 832(C).T cells. miR‑148a downregulation using miR inhibitor significantly increased cell viability, inhibited apoptosis, and reduced the Bax/Bcl‑2 ratio and caspase3/9 activity, and induced HLA‑G protein expression in Hs 832(C).T cells. The GPER inhibitor, G15, suppressed GPER protein expression, upregulated miR‑148a expression, decreased cell proliferation, promoted apoptosis, increased the Bax/Bcl‑2 ratio and caspase3 activity, and suppressed HLA‑G protein expression in Hs 832(C).T cells. The findings indicate that GPER/miR‑148a/HLA‑G signaling pathway may mediates the development of ovarian endometriosis and may become a potential therapeutic target for the treatment of endometriosis.
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1 

Chen M, Huang L, Zhang W, Shi J, Lin X, Lv Z, Zhang W, Liang R and Jiang S: MiR-23b controls TGF-β1 induced airway smooth muscle cell proliferation via TGFβR2/p-Smad3 signals. Mol Immunol. 70:84–93. 2016. View Article : Google Scholar : PubMed/NCBI

2 

Zhu S, Pan W, Song X, Liu Y, Shao X, Tang Y, Liang D, He D, Wang H, Liu W, et al: The microRNA miR-23b suppresses IL-17-associated autoimmune inflammation by targeting TAB2, TAB3 and IKK-α. Nat Med. 18:1077–1086. 2012. View Article : Google Scholar : PubMed/NCBI

3 

Oguiza A, Recio C, Lazaro I, Mallavia B, Blanco J, Egido J and Gomez-Guerrero C: Peptide-based inhibition of IκB kinase/nuclear factor-κB pathway protects against diabetes-associated nephropathy and atherosclerosis in a mouse model of type 1 diabetes. Diabetologia. 58:1656–1667. 2015. View Article : Google Scholar : PubMed/NCBI

4 

Ahmed S, Mundhe N, Borgohain M, Chowdhury L, Kwatra M, Bolshette N, Ahmed A and Lahkar M: Diosmin modulates the NF-kB signal transduction pathways and downregulation of various oxidative stress markers in alloxan-induced diabetic nephropath. Inflammation. 39:1783–1797. 2016. View Article : Google Scholar : PubMed/NCBI

5 

Yu GY, Zheng GZ, Chang B, Hu QX, Lin FX, Liu DZ, Wu CC, Du SX and Li XD: Naringin stimulates osteogenic differentiation of rat bone marrow stromal cells via activation of the notch signaling pathway. Stem Cells Int. 2016:71306532016. View Article : Google Scholar : PubMed/NCBI

6 

Crane CA, Han SJ, Ahn B, Oehlke J, Kivett V, Fedoroff A, Butowski N, Chang SM, Clarke J, Berger MS, et al: Individual patient-specific immunity against high-grade glioma after vaccination with autologous tumor derived peptides bound to the 96 KD chaperone protein. Clin Cancer Res. 19:205–214. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Pan E, Yu D, Yue B, Potthast L, Chowdhary S, Smith P and Chamberlain M: A prospective phase II single-institution trial of sunitinib for recurrent malignant glioma. J Neurooncol. 110:111–118. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Buckner JC, Shaw EG, Pugh SL, Chakravarti A, Gilbert MR, Barger GR, Coons S, Ricci P, Bullard D, Brown PD, et al: Radiation plus procarbazine, CCNU and vincristine in low-grade glioma. N Engl J Med. 374:1344–1355. 2016. View Article : Google Scholar : PubMed/NCBI

9 

Karremann M, Hoffmann M, Benesch M, Kwiecien R, von Bueren AO and Kramm CM: Secondary solid malignancies after high-grade glioma treatment in pediatric patients. Pediatr Hematol Oncol. 32:467–473. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Ducassou A, Uro-Coste E, Verrelle P, Filleron T, Benouaich-Amiel A, Lubrano V, Sol JC, Delisle MB, Favre G, Ken S, et al: αvβ3 Integrin and Fibroblast growth factor receptor 1 (FGFR1): Prognostic factors in a phase I–II clinical trial associating continuous administration of Tipifarnib with radiotherapy for patients with newly diagnosed glioblastoma. Eur J Cancer. 49:2161–2169. 2013. View Article : Google Scholar : PubMed/NCBI

11 

Bischof J, Westhoff MA, Wagner JE, Halatsch ME, Trentmann S, Knippschild U, Wirtz CR and Burster T: Cancer stem cells: The potential role of autophagy, proteolysis, and cathepsins in glioblastoma stem cells. Tumour Biol. 39:10104283176922272017. View Article : Google Scholar : PubMed/NCBI

12 

Mazurowski MA, Clark K, Czarnek NM, Shamsesfandabadi P, Peters KB and Saha A: Radiogenomics of lower-grade glioma: Algorithmically-assessed tumor shape is associated with tumor genomic subtypes and patient outcomes in a multi-institutional study with the cancer genome atlas data. J Neurooncol. 133:27–35. 2017. View Article : Google Scholar : PubMed/NCBI

13 

Hernández-Tiedra S, Fabriàs G, Dávila D, Salanueva ÍJ, Casas J, Montes LR, Antón Z, García-Taboada E, Salazar-Roa M, Lorente M, et al: Dihydroceramide accumulation mediates cytotoxic autophagy of cancer cells via autolysosome destabilization. Autophagy. 12:2213–2229. 2016. View Article : Google Scholar : PubMed/NCBI

14 

Quinlan S, Kenny A, Medina M, Engel T and Jimenez-Mateos EM: MicroRNAs in neurodegenerative diseases. Int Rev Cell Mol Biol. 334:309–343. 2017. View Article : Google Scholar : PubMed/NCBI

15 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

16 

Ohga S, Shikata K, Yozai K, Okada S, Ogawa D, Usui H, Wada J, Shikata Y and Makino H: Thiazolidinedione ameliorates renal injury in experimental diabetic rats through anti-inflammatory effects mediated by inhibition of NF-kappaB activation. Am J Physiol Renal Physiol. 292:F1141–F1150. 2007. View Article : Google Scholar : PubMed/NCBI

17 

Jiang Q, Liu P, Wu X, Liu W, Shen X, Lan T, Xu S, Peng J, Xie X and Huang H: Berberine attenuates lipopolysaccharide-induced extracelluar matrix accumulation and inflammation in rat mesangial cells: Involvement of NF-κB signaling pathway. Mol Cell Endocrinol. 331:34–40. 2011. View Article : Google Scholar : PubMed/NCBI

18 

Li HL, Cui XL, Zhang JN and Lin S: Chemotherapy alleviates subacute recurrent glioma-associated refractory cerebral edema by downregulating vascular endothelial growth factor. Med Oncol. 31:132014. View Article : Google Scholar : PubMed/NCBI

19 

Bartels U, Wolff J, Gore L, Dunkel I, Gilheeney S, Allen J, Goldman S, Yalon M, Packer RJ, Korones DN, et al: Phase 2 study of safety and efficacy of nimotuzumab in pediatric patients with progressive diffuse intrinsic pontine glioma. Neuro Oncol. 16:1554–1559. 2014. View Article : Google Scholar : PubMed/NCBI

20 

Luo X, Zheng X and Huang H: Protective effects of dexmedetomidine on brain function of glioma patients undergoing craniotomy resection and its underlying mechanism. Clin Neurol Neurosurg. 146:105–108. 2016. View Article : Google Scholar : PubMed/NCBI

21 

Chen CM, Syu JP, Way TD, Huang LJ, Kuo SC, Lin CT and Lin CL: BC3EE2,9B, a synthetic carbazole derivative, upregulates autophagy and synergistically sensitizes human GBM8901 glioblastoma cells to temozolomide. Int J Mol Med. 36:1244–1252. 2015. View Article : Google Scholar : PubMed/NCBI

22 

Ding X, Ma M, Teng J, Teng RK, Zhou S, Yin J, Fonkem E, Huang JH, Wu E and Wang X: Exposure to ALS-FTD-CSF generates TDP-43 aggregates in glioblastoma cells through exosomes and TNTs-like structure. Oncotarget. 6:24178–24191. 2015. View Article : Google Scholar : PubMed/NCBI

23 

Lin CJ, Chen TL, Tseng YY, Wu GJ, Hsieh MH, Lin YW and Chen RM: Honokiol induces autophagic cell death in malignant glioma through reactive oxygen species-mediated regulation of the p53/PI3K/Akt/mTOR signaling pathway. Toxicol Appl Pharmacol. 304:59–69. 2016. View Article : Google Scholar : PubMed/NCBI

24 

Li P, Wang X, Shan Q, Wu Y and Wang Z: MicroRNA-130b promotes cell migration and invasion by inhibiting peroxisome proliferator-activated receptor-γ in human glioma. Oncol Lett. 13:2615–2622. 2017. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
He SZ, Li J, Bao HC, Wang MM, Wang XR, Huang X, Li FH, Zhang W, Xu AL, Fang HC, Fang HC, et al: G protein‑coupled estrogen receptor/miR‑148a/human leukocyte antigen‑G signaling pathway mediates cell apoptosis of ovarian endometriosis. Mol Med Rep 18: 1141-1148, 2018.
APA
He, S.Z., Li, J., Bao, H.C., Wang, M.M., Wang, X.R., Huang, X. ... Sheng, Y.X. (2018). G protein‑coupled estrogen receptor/miR‑148a/human leukocyte antigen‑G signaling pathway mediates cell apoptosis of ovarian endometriosis. Molecular Medicine Reports, 18, 1141-1148. https://doi.org/10.3892/mmr.2018.9039
MLA
He, S. Z., Li, J., Bao, H. C., Wang, M. M., Wang, X. R., Huang, X., Li, F. H., Zhang, W., Xu, A. L., Fang, H. C., Sheng, Y. X."G protein‑coupled estrogen receptor/miR‑148a/human leukocyte antigen‑G signaling pathway mediates cell apoptosis of ovarian endometriosis". Molecular Medicine Reports 18.1 (2018): 1141-1148.
Chicago
He, S. Z., Li, J., Bao, H. C., Wang, M. M., Wang, X. R., Huang, X., Li, F. H., Zhang, W., Xu, A. L., Fang, H. C., Sheng, Y. X."G protein‑coupled estrogen receptor/miR‑148a/human leukocyte antigen‑G signaling pathway mediates cell apoptosis of ovarian endometriosis". Molecular Medicine Reports 18, no. 1 (2018): 1141-1148. https://doi.org/10.3892/mmr.2018.9039
Copy and paste a formatted citation
x
Spandidos Publications style
He SZ, Li J, Bao HC, Wang MM, Wang XR, Huang X, Li FH, Zhang W, Xu AL, Fang HC, Fang HC, et al: G protein‑coupled estrogen receptor/miR‑148a/human leukocyte antigen‑G signaling pathway mediates cell apoptosis of ovarian endometriosis. Mol Med Rep 18: 1141-1148, 2018.
APA
He, S.Z., Li, J., Bao, H.C., Wang, M.M., Wang, X.R., Huang, X. ... Sheng, Y.X. (2018). G protein‑coupled estrogen receptor/miR‑148a/human leukocyte antigen‑G signaling pathway mediates cell apoptosis of ovarian endometriosis. Molecular Medicine Reports, 18, 1141-1148. https://doi.org/10.3892/mmr.2018.9039
MLA
He, S. Z., Li, J., Bao, H. C., Wang, M. M., Wang, X. R., Huang, X., Li, F. H., Zhang, W., Xu, A. L., Fang, H. C., Sheng, Y. X."G protein‑coupled estrogen receptor/miR‑148a/human leukocyte antigen‑G signaling pathway mediates cell apoptosis of ovarian endometriosis". Molecular Medicine Reports 18.1 (2018): 1141-1148.
Chicago
He, S. Z., Li, J., Bao, H. C., Wang, M. M., Wang, X. R., Huang, X., Li, F. H., Zhang, W., Xu, A. L., Fang, H. C., Sheng, Y. X."G protein‑coupled estrogen receptor/miR‑148a/human leukocyte antigen‑G signaling pathway mediates cell apoptosis of ovarian endometriosis". Molecular Medicine Reports 18, no. 1 (2018): 1141-1148. https://doi.org/10.3892/mmr.2018.9039
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