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Article Open Access

Quercetin protects against ox‑LDL‑induced injury via regulation of ABCAl, LXR‑α and PCSK9 in RAW264.7 macrophages

  • Authors:
    • Shanshan Li
    • Hui Cao
    • Dingzhu Shen
    • Qingling Jia
    • Chuan Chen
    • San Li Xing
  • View Affiliations / Copyright

    Affiliations: Shanghai Geriatrics Institute of Chinese Medicine, Shanghai 200031, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 799-806
    |
    Published online on: May 22, 2018
       https://doi.org/10.3892/mmr.2018.9048
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Abstract

Quercetin is a flavonoid that has anti‑inflammatory, anti‑oxidant and lipid metabolic effects. It has also been reported to reduce the risk of cardiovascular disease. The present study measured the effects of quercetin on the expression of ATP‑binding cassette transporter 1 (ABCAl), ATP‑binding cassette sub‑family G member 1 (ABCG1), liver X receptor‑α (LXR‑α), proprotein convertase subtilisin/kexin type 9 (PCSK9), p53, p21 and p16 induced by oxidized low density lipoprotein (ox‑LDL). RAW264.7 macrophages were exposed to ox‑LDL with or without 20 µmol/l quercetin and cell proliferation and senescence were quantified using β‑gal staining. Superoxide dismutase (SOD), malondialdehyde (MDA) and lipid droplets were measured in the cytoplasm using oil red staining, while intracellular and total cholesterol (TC) were measured using filipin staining and a TC kit. Immunofluorescent studies and western blot analysis were performed to quantify the expression of ABCAl, ABCG1, LXR‑α, PCSK9, p53, p21 and p16. Quercetin increased RAW264.7 cell viability and reduced lipid accumulation, senescence, lipid droplets, intracellular cholesterol and TC. It was concluded that quercetin inhibits ox‑LDL‑induced lipid droplets in RAW264.7 cells by upregulation of ABCAl, ABCG1, LXR‑α and downregulation of PCSK9, p53, p21 and p16.
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Copy and paste a formatted citation
Spandidos Publications style
Li S, Cao H, Shen D, Jia Q, Chen C and Xing SL: Quercetin protects against ox‑LDL‑induced injury via regulation of ABCAl, LXR‑α and PCSK9 in RAW264.7 macrophages. Mol Med Rep 18: 799-806, 2018.
APA
Li, S., Cao, H., Shen, D., Jia, Q., Chen, C., & Xing, S.L. (2018). Quercetin protects against ox‑LDL‑induced injury via regulation of ABCAl, LXR‑α and PCSK9 in RAW264.7 macrophages. Molecular Medicine Reports, 18, 799-806. https://doi.org/10.3892/mmr.2018.9048
MLA
Li, S., Cao, H., Shen, D., Jia, Q., Chen, C., Xing, S. L."Quercetin protects against ox‑LDL‑induced injury via regulation of ABCAl, LXR‑α and PCSK9 in RAW264.7 macrophages". Molecular Medicine Reports 18.1 (2018): 799-806.
Chicago
Li, S., Cao, H., Shen, D., Jia, Q., Chen, C., Xing, S. L."Quercetin protects against ox‑LDL‑induced injury via regulation of ABCAl, LXR‑α and PCSK9 in RAW264.7 macrophages". Molecular Medicine Reports 18, no. 1 (2018): 799-806. https://doi.org/10.3892/mmr.2018.9048
Copy and paste a formatted citation
x
Spandidos Publications style
Li S, Cao H, Shen D, Jia Q, Chen C and Xing SL: Quercetin protects against ox‑LDL‑induced injury via regulation of ABCAl, LXR‑α and PCSK9 in RAW264.7 macrophages. Mol Med Rep 18: 799-806, 2018.
APA
Li, S., Cao, H., Shen, D., Jia, Q., Chen, C., & Xing, S.L. (2018). Quercetin protects against ox‑LDL‑induced injury via regulation of ABCAl, LXR‑α and PCSK9 in RAW264.7 macrophages. Molecular Medicine Reports, 18, 799-806. https://doi.org/10.3892/mmr.2018.9048
MLA
Li, S., Cao, H., Shen, D., Jia, Q., Chen, C., Xing, S. L."Quercetin protects against ox‑LDL‑induced injury via regulation of ABCAl, LXR‑α and PCSK9 in RAW264.7 macrophages". Molecular Medicine Reports 18.1 (2018): 799-806.
Chicago
Li, S., Cao, H., Shen, D., Jia, Q., Chen, C., Xing, S. L."Quercetin protects against ox‑LDL‑induced injury via regulation of ABCAl, LXR‑α and PCSK9 in RAW264.7 macrophages". Molecular Medicine Reports 18, no. 1 (2018): 799-806. https://doi.org/10.3892/mmr.2018.9048
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