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Article

Effect of co‑culture with amniotic epithelial cells on the biological characteristics of amniotic mesenchymal stem cells

  • Authors:
    • Li‑Jing Ran
    • Yun Zeng
    • Shao‑Chun Wang
    • Di‑Si Zhang
    • Min Hong
    • Shao‑You Li
    • Jian Dong
    • Ming‑Xia Shi
  • View Affiliations / Copyright

    Affiliations: Department of Hematology, The First Affiliated Hospital of Kunming Medical University, Hematology Research Center of Yunnan Province, Kunming, Yunnan 650032, P.R. China, Department of Ultrasonography, The Affiliated Hospital of Jining Medical College, Jining, Shandong 272000, P.R. China, Department of Oncology, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650032, P.R. China
    Copyright: © Ran et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 723-732
    |
    Published online on: May 23, 2018
       https://doi.org/10.3892/mmr.2018.9053
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Abstract

The aim of the present study was to investigate the effect of co‑culture with amniotic epithelial cells (AECs) on the biological characteristics of amniotic mesenchymal stem cells (AMSCs), to compare the expression of C‑X‑C motif chemokine receptor 4 (CXCR4) in co‑cultured AMSCs and to investigate the roles of the stromal cell‑derived factor‑1 (SDF‑1)/CXCR4 axis in the homing and migration of AMSCs. AMSCs were isolated from human amniotic membranes, purified and then differentiated into osteoblasts and adipocytes in vitro, which was verified by von Kossa Staining and Oil Red O staining. Cell viability was measured by Cell Counting kit‑8 and trypan blue assays at 24, 48 and 72 h, the expression of CXCR4 was analyzed by immunofluorescence‑based flow cytometry and reverse transcription‑quantitative polymerase chain reaction, and the migration ability of AMSCs in vitro was observed by a migration assay. The results demonstrated that cell viability (at 48 and 72 h) and survival (at 24, 48 and 72 h) in the co‑culture and serum groups were higher compared with the serum‑free group. Furthermore, CXCR4 mRNA and protein expression, and migration along the SDF‑1 gradient, in the co‑culture and serum‑free groups were higher compared with the serum group. Overall, the results indicated that AMSCs co‑cultured with AECs exhibited enhanced proliferation activity and survival rate. In conclusion, the present study demonstrated that co‑culture of AMSCs with AECs upregulated CXCR4 on the surface of AMSCs and enhanced the migration ability of AMSCs in vitro. This result may improve the directional migration and homing ability of AMSCs, as well as provide a theoretical basis for the application of AMSCs in clinical practice as a novel strategy to increase the success of hematopoietic stem cell transplantation.
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View References

1 

Castro-Manrreza ME and Montesinos JJ: Immunoregulation by mesenchymal stem cells: Biological aspects and clinical applications. J Immunol Res. 2015:3949172015. View Article : Google Scholar : PubMed/NCBI

2 

Bernardo ME and Locatelli F: Mesenchymal stromal cells in hematopoietic stem cell transplantation. Methods Mol Biol. 1416:3–20. 2016. View Article : Google Scholar : PubMed/NCBI

3 

Larsen S and Lewis ID: Potential therapeutic applications of mesenchymal stromal cells. Pathology. 43:592–604. 2011. View Article : Google Scholar : PubMed/NCBI

4 

Manuelpillai U, Moodley Y, Borlongan CV and Parolini O: Amniotic membrane and amniotic cells: Potential therapeutic tools to combat tissue inflammation and fibrosis? Placenta. 32 Suppl 4:S320–S325. 2011. View Article : Google Scholar : PubMed/NCBI

5 

Shi MX, Fang BJ, Liao LM, Yang SG, Liu YH and Zhao CH: Flk1+ mesenchymal stem cells ameliorate carbon tetrachloride-induced liver fibrosis in mice. Sheng Wu Gong Cheng Xue Bao. 21:396–401. 2005.(In Chinese). PubMed/NCBI

6 

Wu Y, Wang Z, Cao Y, Xu L, Li X, Liu P, Yan P, Liu Z, Zhao D, Wang J, et al: Cotransplantation of haploidentical hematopoietic and umbilical cord mesenchymal stem cells with a myeloablative regimen for refractory/relapsed hematologic malignancy. Ann Hematol. 92:1675–1684. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Nakamura K, Inaba M, Sugiura K, Yoshimura T, Kwon AH, Kamiyama Y and Ikehara S: Enhancement of allogeneic hematopoietic stem cell engraftment and prevention of GVHD by intra-bone marrow bone marrow transplantation plus donor lymphocyte infusion. Stem Cells. 22:125–134. 2004. View Article : Google Scholar : PubMed/NCBI

8 

Shi M, Li J, Liao L, Chen B, Li B, Chen L, Jia H and Zhao RC: Regulation of CXCR4 expression in human mesenchymal stem cells by cytokine treatment: role in homing efficiency in NOD/SCID mice. Haematologica. 92:897–904. 2007. View Article : Google Scholar : PubMed/NCBI

9 

Gong J, Meng HB, Hua J, Song ZS, He ZG, Zhou B and Qian MP: The SDF-1/CXCR4 axis regulates migration of transplanted bone marrow mesenchymal stem cells towards the pancreas in rats with acute pancreatitis. Mol Med Rep. 9:1575–1582. 2014. View Article : Google Scholar : PubMed/NCBI

10 

Rider P, Carmi Y and Cohen I: Biologics for targeting inflammatory cytokines, clinical uses, and limitations. Int J Cell Biol. 2016:92596462016. View Article : Google Scholar : PubMed/NCBI

11 

Yang S, Sun HM, Yan JH, Xue H, Wu B, Dong F, Li WS, Ji FQ and Zhou DS: Conditioned medium from human amniotic epithelial cells may induce the differentiation of human umbilical cord blood mesenchymal stem cells into dopaminergic neuron-like cells. J Neurosci Res. 91:978–986. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Tabatabaei M, Mosaffa N, Nikoo S, Bozorgmehr M, Ghods R, Kazemnejad S, Rezania S, Keshavarzi B, Arefi S, Ramezani-Tehrani F, et al: Isolation and partial characterization of human amniotic epithelial cells: The effect of trypsin. Avicenna J Med Biotechnol. 6:10–20. 2014.PubMed/NCBI

13 

Fang B, Shi M, Liao L, Yang S, Liu Y and Zhao RC: Multiorgan engraftment and multilineage differentiation by human fetal bone marrow Flk1+/CD31-/CD34-Progenitors. J Hematother Stem Cell Res. 12:603–613. 2003. View Article : Google Scholar : PubMed/NCBI

14 

Sawa M, Inoue M, Yabuki A, Kohyama M, Miyoshi N, Setoguchi A and Yamato O: Rapid immunocytochemistry for the detection of cytokeratin and vimentin: Assessment of its diagnostic value in neoplastic diseases of dogs. J Vet Clin Pathol. 46:172–178. 2017. View Article : Google Scholar

15 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

16 

Shi M, Li W, Li B, Li J and Zhao C: Multipotency of adult stem cells derived from human amnion. Sheng Wu Gong Cheng Xue Bao. 25:754–760. 2009.(In Chinese). PubMed/NCBI

17 

Vojdani Z, Babaei A, Vasaghi A, Habibagahi M and Talaei-Khozani T: The effect of amniotic membrane extract on umbilical cord blood mesenchymal stem cell expansion: Is there any need to save the amniotic membrane besides the umbilical cord blood? Iran J Basic Med Sci. 19:89–96. 2016.PubMed/NCBI

18 

Capobianco V, Caterino M, Iaffaldano L, Nardelli C, Sirico A, Del Vecchio L, Martinelli P, Pastore L, Pucci P and Sacchetti L: Proteome analysis of human amniotic mesenchymal stem cells (hA-MSCs) reveals impaired antioxidant ability, cytoskeleton and metabolic functionality in maternal obesity. Sci Rep. 6:252702016. View Article : Google Scholar : PubMed/NCBI

19 

Zhou H, Zhang H, Yan Z and Xu R: Transplantation of human amniotic mesenchymal stem cells promotes neurological recovery in an intracerebral hemorrhage rat model. Biochem Biophys Res Commun. 475:202–208. 2016. View Article : Google Scholar : PubMed/NCBI

20 

Herrmann RP and Sturm MJ: Adult human mesenchymal stromal cells and the treatment of graft versus host disease. Stem Cells Cloning. 7:45–52. 2014.PubMed/NCBI

21 

Belmar-Lopez C, Mendoza G, Oberg D, Burnet J, Simon C, Cervello I, Iglesias M, Ramirez JC, Lopez-Larrubia P, Quintanilla M, Martin-Duque P, et al: Tissue-derived mesenchymal stromal cells used as vehicles for anti-tumor therapy exert different in vivo effects on migration capacity and tumor growth. BMC Med. 11:1392013. View Article : Google Scholar : PubMed/NCBI

22 

Sharma M, Afrin F, Tripathi R and Gangenahalli G: Regulated expression of CXCR4 constitutive active mutants revealed the up-modulated chemotaxis and up-regulation of genes crucial for CXCR4 mediated homing and engraftment of hematopoietic stem/progenitor cells. J Stem Cells Regen Med. 9:19–27. 2013.PubMed/NCBI

23 

Li J, Guo W, Xiong M, Han H, Chen J, Mao D, Tang B, Yu H and Zeng Y: Effect of SDF-1/CXCR4 axis on the migration of transplanted bone mesenchymal stem cells mobilized by erythropoietin toward lesion sites following spinal cord injury. Int J Mol Med. 36:1205–1214. 2015. View Article : Google Scholar : PubMed/NCBI

24 

Díaz-Prado S, Muiños-López E, Hermida-Gómez T, Rendal-Vázquez ME, Fuentes-Boquete I, de Toro FJ and Blanco FJ: Multilineage differentiation potential of cells isolated from the human amniotic membrane. J Cell Biochem. 111:846–857. 2010. View Article : Google Scholar : PubMed/NCBI

25 

Pelekanos RA, Ting MJ, Sardesai VS, Ryan JM, Lim YC, Chan JK and Fisk NM: Intracellular trafficking and endocytosis of CXCR4 in fetal mesenchymal stem/stromal cells. BMC Cell Biol. 15:152014. View Article : Google Scholar : PubMed/NCBI

26 

Li Y, Yu X, Lin S, Li X, Zhang S and Song YH: Insulin-like growth factor 1 enhances the migratory capacity of mesenchymal stem cells. Biochem Biophys Res Commun. 356:780–784. 2007. View Article : Google Scholar : PubMed/NCBI

27 

Li M, Yu J, Li Y, Li D, Yan D, Qu Z and Ruan Q: CXCR4 positive bone mesenchymal stem cells migrate to human endothelial cell stimulated by ox-LDL via SDF-1alpha/CXCR4 signaling axis. Exp Mol Pathol. 88:250–255. 2010. View Article : Google Scholar : PubMed/NCBI

28 

Hung CM, Hsu YC, Chen TY, Chang CC and Lee MJ: Cyclophosphamide promotes breast cancer cell migration through CXCR4 and matrix metalloproteinases. Cell Biol Int. 41:345–352. 2017. View Article : Google Scholar : PubMed/NCBI

29 

Sheng X, Zhong H, Wan H, Zhong J and Chen F: Granulocyte colony-stimulating factor inhibits CXCR4/SDF-1α signaling and overcomes stromal-mediated drug resistance in the HL-60 cell line. Exp Ther Med. 12:396–404. 2016. View Article : Google Scholar : PubMed/NCBI

30 

Shen W, Chen J, Zhu T, Chen L, Zhang W, Fang Z, Heng BC, Yin Z, Chen X, Ji J, et al: Intra-articular injection of human meniscus stem/progenitor cells promotes meniscus regeneration and ameliorates osteoarthritis through stromal cell-derived factor-1/CXCR4-mediated homing. Stem Cells Transl Med. 3:387–394. 2014. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Ran LJ, Zeng Y, Wang SC, Zhang DS, Hong M, Li SY, Dong J and Shi MX: Effect of co‑culture with amniotic epithelial cells on the biological characteristics of amniotic mesenchymal stem cells. Mol Med Rep 18: 723-732, 2018.
APA
Ran, L., Zeng, Y., Wang, S., Zhang, D., Hong, M., Li, S. ... Shi, M. (2018). Effect of co‑culture with amniotic epithelial cells on the biological characteristics of amniotic mesenchymal stem cells. Molecular Medicine Reports, 18, 723-732. https://doi.org/10.3892/mmr.2018.9053
MLA
Ran, L., Zeng, Y., Wang, S., Zhang, D., Hong, M., Li, S., Dong, J., Shi, M."Effect of co‑culture with amniotic epithelial cells on the biological characteristics of amniotic mesenchymal stem cells". Molecular Medicine Reports 18.1 (2018): 723-732.
Chicago
Ran, L., Zeng, Y., Wang, S., Zhang, D., Hong, M., Li, S., Dong, J., Shi, M."Effect of co‑culture with amniotic epithelial cells on the biological characteristics of amniotic mesenchymal stem cells". Molecular Medicine Reports 18, no. 1 (2018): 723-732. https://doi.org/10.3892/mmr.2018.9053
Copy and paste a formatted citation
x
Spandidos Publications style
Ran LJ, Zeng Y, Wang SC, Zhang DS, Hong M, Li SY, Dong J and Shi MX: Effect of co‑culture with amniotic epithelial cells on the biological characteristics of amniotic mesenchymal stem cells. Mol Med Rep 18: 723-732, 2018.
APA
Ran, L., Zeng, Y., Wang, S., Zhang, D., Hong, M., Li, S. ... Shi, M. (2018). Effect of co‑culture with amniotic epithelial cells on the biological characteristics of amniotic mesenchymal stem cells. Molecular Medicine Reports, 18, 723-732. https://doi.org/10.3892/mmr.2018.9053
MLA
Ran, L., Zeng, Y., Wang, S., Zhang, D., Hong, M., Li, S., Dong, J., Shi, M."Effect of co‑culture with amniotic epithelial cells on the biological characteristics of amniotic mesenchymal stem cells". Molecular Medicine Reports 18.1 (2018): 723-732.
Chicago
Ran, L., Zeng, Y., Wang, S., Zhang, D., Hong, M., Li, S., Dong, J., Shi, M."Effect of co‑culture with amniotic epithelial cells on the biological characteristics of amniotic mesenchymal stem cells". Molecular Medicine Reports 18, no. 1 (2018): 723-732. https://doi.org/10.3892/mmr.2018.9053
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