Hypoxia induces lactate secretion and glycolytic efflux by downregulating mitochondrial pyruvate carrier levels in human umbilical vein endothelial cells

  • Authors:
    • Dong Wang
    • Qingjie Wang
    • Gaoliang Yan
    • Yong Qiao
    • Boqian Zhu
    • Bo Liu
    • Chengchun Tang
  • View Affiliations

  • Published online on: May 29, 2018     https://doi.org/10.3892/mmr.2018.9079
  • Pages: 1710-1717
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Abstract

The mitochondrial pyruvate carrier (MPC) complex, located on the inner mitochondrial membrane, transports pyruvate to the mitochondrial matrix for oxidative phosphorylation. Previous studies have shown that the MPC complex is a key regulator of glycolysis in tumor cells. The present study evaluated the role of the MPC under hypoxic conditions in human umbilical vein endothelial cells, which rely on glycolysis for energy generation. It was indicated that hypoxia led to an increase in lactate secretion and a decrease in MPC1 and MPC2 levels, which were upregulated following re‑oxygenation. In addition, the knockdown of MPC1 or treatment with the MPC inhibitor UK5099 increased the levels of glycolytic enzymes, HK2, PFKFB3, and LDHA, promoting glycolysis and lactate secretion. Taken together, the present data revealed that hypoxia can induce lactate secretion and glycolytic efflux by downregulating MPC levels.
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August-2018
Volume 18 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Wang D, Wang Q, Yan G, Qiao Y, Zhu B, Liu B and Tang C: Hypoxia induces lactate secretion and glycolytic efflux by downregulating mitochondrial pyruvate carrier levels in human umbilical vein endothelial cells. Mol Med Rep 18: 1710-1717, 2018
APA
Wang, D., Wang, Q., Yan, G., Qiao, Y., Zhu, B., Liu, B., & Tang, C. (2018). Hypoxia induces lactate secretion and glycolytic efflux by downregulating mitochondrial pyruvate carrier levels in human umbilical vein endothelial cells. Molecular Medicine Reports, 18, 1710-1717. https://doi.org/10.3892/mmr.2018.9079
MLA
Wang, D., Wang, Q., Yan, G., Qiao, Y., Zhu, B., Liu, B., Tang, C."Hypoxia induces lactate secretion and glycolytic efflux by downregulating mitochondrial pyruvate carrier levels in human umbilical vein endothelial cells". Molecular Medicine Reports 18.2 (2018): 1710-1717.
Chicago
Wang, D., Wang, Q., Yan, G., Qiao, Y., Zhu, B., Liu, B., Tang, C."Hypoxia induces lactate secretion and glycolytic efflux by downregulating mitochondrial pyruvate carrier levels in human umbilical vein endothelial cells". Molecular Medicine Reports 18, no. 2 (2018): 1710-1717. https://doi.org/10.3892/mmr.2018.9079