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Article Open Access

miR‑23a downregulation modulates the inflammatory response by targeting ATG12‑mediated autophagy

  • Authors:
    • Xiang Si
    • Daiyin Cao
    • Juan Chen
    • Yao Nie
    • Zhiyi Jiang
    • Min‑Ying Chen
    • Jian‑Feng Wu
    • Xiang‑Dong Guan
  • View Affiliations / Copyright

    Affiliations: Department of SICU, The First Affiliated Hospital of Sun Yat‑sen University, Guangzhou, Guangdong 510000, P.R. China, Department of Critical Care Medicine, The Sixth Affiliated Hospital of Sun Yat‑sen University, Guangzhou, Guangdong 510000, P.R. China
    Copyright: © Si et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1524-1530
    |
    Published online on: May 29, 2018
       https://doi.org/10.3892/mmr.2018.9081
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Abstract

Autophagy, part of the innate immune defense mechanisms, is activated during the initial phase of septic insult. Previous studies indicated that micro (mi)RNAs are additionally involved in the host response to sepsis; however, the association between miRNAs and autophagy during this process is not fully understood. To study the role of miRNA (miR)‑23a in autophagy initiated by sepsis, macrophages treated with lipopolysaccharides, in addition to blood samples from patients, were evaluated for miR‑23a expression levels. Cell viability, inflammatory mediators and autophagic markers were investigated following overexpression or inhibition of miR‑23a. The results suggested that miR‑23a was suppressed subsequent to septic insult, promoting autophagy and suppressing a hyper inflammatory response, leading to enhanced cell viability. A luciferase assay and western blot analysis confirmed ubiquitin‑like protein ATG12 to be the target of miR‑23a. The present study revealed that the downregulation of miR‑23a regulates an inflammatory response during septic insult via autophagy promotion.
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Copy and paste a formatted citation
Spandidos Publications style
Si X, Cao D, Chen J, Nie Y, Jiang Z, Chen MY, Wu JF and Guan XD: miR‑23a downregulation modulates the inflammatory response by targeting ATG12‑mediated autophagy. Mol Med Rep 18: 1524-1530, 2018.
APA
Si, X., Cao, D., Chen, J., Nie, Y., Jiang, Z., Chen, M. ... Guan, X. (2018). miR‑23a downregulation modulates the inflammatory response by targeting ATG12‑mediated autophagy. Molecular Medicine Reports, 18, 1524-1530. https://doi.org/10.3892/mmr.2018.9081
MLA
Si, X., Cao, D., Chen, J., Nie, Y., Jiang, Z., Chen, M., Wu, J., Guan, X."miR‑23a downregulation modulates the inflammatory response by targeting ATG12‑mediated autophagy". Molecular Medicine Reports 18.2 (2018): 1524-1530.
Chicago
Si, X., Cao, D., Chen, J., Nie, Y., Jiang, Z., Chen, M., Wu, J., Guan, X."miR‑23a downregulation modulates the inflammatory response by targeting ATG12‑mediated autophagy". Molecular Medicine Reports 18, no. 2 (2018): 1524-1530. https://doi.org/10.3892/mmr.2018.9081
Copy and paste a formatted citation
x
Spandidos Publications style
Si X, Cao D, Chen J, Nie Y, Jiang Z, Chen MY, Wu JF and Guan XD: miR‑23a downregulation modulates the inflammatory response by targeting ATG12‑mediated autophagy. Mol Med Rep 18: 1524-1530, 2018.
APA
Si, X., Cao, D., Chen, J., Nie, Y., Jiang, Z., Chen, M. ... Guan, X. (2018). miR‑23a downregulation modulates the inflammatory response by targeting ATG12‑mediated autophagy. Molecular Medicine Reports, 18, 1524-1530. https://doi.org/10.3892/mmr.2018.9081
MLA
Si, X., Cao, D., Chen, J., Nie, Y., Jiang, Z., Chen, M., Wu, J., Guan, X."miR‑23a downregulation modulates the inflammatory response by targeting ATG12‑mediated autophagy". Molecular Medicine Reports 18.2 (2018): 1524-1530.
Chicago
Si, X., Cao, D., Chen, J., Nie, Y., Jiang, Z., Chen, M., Wu, J., Guan, X."miR‑23a downregulation modulates the inflammatory response by targeting ATG12‑mediated autophagy". Molecular Medicine Reports 18, no. 2 (2018): 1524-1530. https://doi.org/10.3892/mmr.2018.9081
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