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Article Open Access

miR‑141 inhibits glioma vasculogenic mimicry by controlling EphA2 expression

  • Authors:
    • Guoxiong Li
    • Min Huang
    • Yingqian Cai
    • Yiquan Ke
    • Yuantao Yang
    • Xinlin Sun
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, People's Hospital of Shiyan, Shenzhen, Guangdong 518108, P.R. China, Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, The National Key Clinical Specialty, The Engineering Technology Research Center of Education Ministry of China, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Guangzhou, Guangdong 510280, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1395-1404
    |
    Published online on: May 31, 2018
       https://doi.org/10.3892/mmr.2018.9108
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Abstract

Human glioma is a pernicious tumor from the central nervous system; it has been reported that microRNAs (miRs) may have carcinogenic or tumor suppressor effects on human glioma. The aim of the present study was to assess miR‑141 expression and functional role in human primary glioma, as well as in tumor‑derived cell lines. The expression of miR‑141 in primary human glioma tissues and cell lines was assessed by employing reverse transcription‑quantitative polymerase chain reaction. Next, its role in cellular growth, migration, invasion and vasculogenic mimicry (VM) regulation was determined using various in vitro and in vivo assays, and on the identification its target gene(s) using luciferase assays. The results demonstrated that miR‑141 expression was downregulated, and Ephrin type‑A receptor 2 (EphA2) was upregulated in the primary human gliomas and human glioma‑derived cell lines tested. In addition, a negative correlation existed between miR‑141 and EphA2 expression levels in glioma grades II, III and IV. Furthermore, exogenous miR‑141 expression resulted in decreased proliferation, migration and invasion, as well as in apoptosis and cell cycle arrest in vitro. It was also revealed that exogenous miR‑141 expression resulted in in vivo inhibition of tumor growth and inhibition of the development of VM. Finally, the present study successfully confirmed that EphA2 was a direct target of miR‑141 in glioma‑derived cells using luciferase assays. Based on these results, it was concluded that miR‑141 may regulate cell proliferation, migration, invasion and VM formation by controlling EphA2 expression; also, its target EphA2 may be a novel diagnostic/prognostic biomarker and a potential anti‑VM therapeutic target.
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Copy and paste a formatted citation
Spandidos Publications style
Li G, Huang M, Cai Y, Ke Y, Yang Y and Sun X: miR‑141 inhibits glioma vasculogenic mimicry by controlling EphA2 expression. Mol Med Rep 18: 1395-1404, 2018.
APA
Li, G., Huang, M., Cai, Y., Ke, Y., Yang, Y., & Sun, X. (2018). miR‑141 inhibits glioma vasculogenic mimicry by controlling EphA2 expression. Molecular Medicine Reports, 18, 1395-1404. https://doi.org/10.3892/mmr.2018.9108
MLA
Li, G., Huang, M., Cai, Y., Ke, Y., Yang, Y., Sun, X."miR‑141 inhibits glioma vasculogenic mimicry by controlling EphA2 expression". Molecular Medicine Reports 18.2 (2018): 1395-1404.
Chicago
Li, G., Huang, M., Cai, Y., Ke, Y., Yang, Y., Sun, X."miR‑141 inhibits glioma vasculogenic mimicry by controlling EphA2 expression". Molecular Medicine Reports 18, no. 2 (2018): 1395-1404. https://doi.org/10.3892/mmr.2018.9108
Copy and paste a formatted citation
x
Spandidos Publications style
Li G, Huang M, Cai Y, Ke Y, Yang Y and Sun X: miR‑141 inhibits glioma vasculogenic mimicry by controlling EphA2 expression. Mol Med Rep 18: 1395-1404, 2018.
APA
Li, G., Huang, M., Cai, Y., Ke, Y., Yang, Y., & Sun, X. (2018). miR‑141 inhibits glioma vasculogenic mimicry by controlling EphA2 expression. Molecular Medicine Reports, 18, 1395-1404. https://doi.org/10.3892/mmr.2018.9108
MLA
Li, G., Huang, M., Cai, Y., Ke, Y., Yang, Y., Sun, X."miR‑141 inhibits glioma vasculogenic mimicry by controlling EphA2 expression". Molecular Medicine Reports 18.2 (2018): 1395-1404.
Chicago
Li, G., Huang, M., Cai, Y., Ke, Y., Yang, Y., Sun, X."miR‑141 inhibits glioma vasculogenic mimicry by controlling EphA2 expression". Molecular Medicine Reports 18, no. 2 (2018): 1395-1404. https://doi.org/10.3892/mmr.2018.9108
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