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Article Open Access

System analysis of teratozoospermia mRNA profile based on integrated bioinformatics tools

  • Authors:
    • Tiancheng Zhang
    • Jun Wu
    • Caihua Liao
    • Zhong Ni
    • Jufen Zheng
    • Fudong Yu
  • View Affiliations / Copyright

    Affiliations: Key Lab of Reproduction Regulation of NPFPC, Shanghai Institute of Planned Parenthood Research, Institute of Reproduction and Development, Fudan University, Shanghai 300000, P.R. China, College of Biotechnology, Guilin Medical University, Guilin, Guangxi Zhuang Autonomous Region 541004, P.R. China, Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu 212013, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1297-1304
    |
    Published online on: May 31, 2018
       https://doi.org/10.3892/mmr.2018.9112
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Abstract

mRNA has an important role in spermatogenesis and the maintenance of fertility, and may act as a potential biomarker for the clinical diagnosis of infertility. In the present study, potential biomarkers associated with teratozoospermia were screened through systemic bioinformatics analysis. Initially, genome‑wide expression profiles were downloaded from the Gene Expression Omnibus and primary analysis was conducted using R software, which included preprocessing of raw microarray data, transformation between probe ID and gene symbol and identification of differentially expressed genes. Subsequently, a functional enrichment analysis was conducted using the Database for Annotation, Visualization and Integrated Discovery to investigate the biological processes involved in the development of teratozoospermia. Finally, a protein‑protein interaction network of notable differentially expressed genes was constructed and cross‑analysis performed for multiple datasets, to obtain a potential biomarker for teratozoospermia. It was observed that G protein subunit β 3, G protein subunit α o1 and G protein subunit g transducin 1 were upregulated and enriched using Kyoto Encyclopedia of Genes and Genomes (KEGG) in the network and in cross analysis. Furthermore, ribosomal protein S3 (RPS3), RPS5, RPS6, RPS16 and RPS23 were downregulated and enriched using KEGG in teratozoospermia. In conclusion, the results of the present study identified several mRNAs involved in sperm morphological development, which may aid in the understanding and treatment of infertility.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang T, Wu J, Liao C, Ni Z, Zheng J and Yu F: System analysis of teratozoospermia mRNA profile based on integrated bioinformatics tools. Mol Med Rep 18: 1297-1304, 2018.
APA
Zhang, T., Wu, J., Liao, C., Ni, Z., Zheng, J., & Yu, F. (2018). System analysis of teratozoospermia mRNA profile based on integrated bioinformatics tools. Molecular Medicine Reports, 18, 1297-1304. https://doi.org/10.3892/mmr.2018.9112
MLA
Zhang, T., Wu, J., Liao, C., Ni, Z., Zheng, J., Yu, F."System analysis of teratozoospermia mRNA profile based on integrated bioinformatics tools". Molecular Medicine Reports 18.2 (2018): 1297-1304.
Chicago
Zhang, T., Wu, J., Liao, C., Ni, Z., Zheng, J., Yu, F."System analysis of teratozoospermia mRNA profile based on integrated bioinformatics tools". Molecular Medicine Reports 18, no. 2 (2018): 1297-1304. https://doi.org/10.3892/mmr.2018.9112
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang T, Wu J, Liao C, Ni Z, Zheng J and Yu F: System analysis of teratozoospermia mRNA profile based on integrated bioinformatics tools. Mol Med Rep 18: 1297-1304, 2018.
APA
Zhang, T., Wu, J., Liao, C., Ni, Z., Zheng, J., & Yu, F. (2018). System analysis of teratozoospermia mRNA profile based on integrated bioinformatics tools. Molecular Medicine Reports, 18, 1297-1304. https://doi.org/10.3892/mmr.2018.9112
MLA
Zhang, T., Wu, J., Liao, C., Ni, Z., Zheng, J., Yu, F."System analysis of teratozoospermia mRNA profile based on integrated bioinformatics tools". Molecular Medicine Reports 18.2 (2018): 1297-1304.
Chicago
Zhang, T., Wu, J., Liao, C., Ni, Z., Zheng, J., Yu, F."System analysis of teratozoospermia mRNA profile based on integrated bioinformatics tools". Molecular Medicine Reports 18, no. 2 (2018): 1297-1304. https://doi.org/10.3892/mmr.2018.9112
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