Assessment of the effects of prostaglandins on myometrial and leiomyoma cells in vitro through microRNA profiling

Kim,Hwi  Gon; Jung,Ga  Young; Park,Seung  Bin; Cho,Yeon  Jean; Han,Myoungseok

doi:10.3892/mmr.2018.9160

August-2018 Volume 18 Issue 2

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August-2018 Volume 18 Issue 2

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Article

Assessment of the effects of prostaglandins on myometrial and leiomyoma cells in vitro through microRNA profiling

Authors:
- Hwi Gon Kim
- Ga Young Jung
- Seung Bin Park
- Yeon Jean Cho
- Myoungseok Han
View Affiliations / Copyright

Affiliations: Department of Obstetrics and Gynecology, Pusan National University Yangsan Hospital, Yangsan, Gyeongsangnam 50612, Republic of Korea, Department of Obstetrics and Gynecology, Dong‑A University, College of Medicine, Busan 49201, Republic of Korea
Pages: 2499-2505
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Published online on: June 14, 2018

https://doi.org/10.3892/mmr.2018.9160
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Abstract

It is well known that prostaglandin (PG) E2 and PGF2α are secreted in copious amounts from the menstruating uterus. The aim of the present study was to determine whether PGs affect the growth of uterine leiomyomas (ULs) to the same extent as estrogen or progesterone (P4). The present study evaluated the expression of eight microRNAs (miRNAs) by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) through treatment with estradiol (E2), P4, PGE2, PGF2α and each antagonist or cyclooxygenase‑2 (COX‑2) inhibitor of cultured leiomyoma and myometrial cells (LC and MC, respectively). The eight miRNAs were divided into two groups according to their primary biological action, namely apoptosis‑regulating miRNAs (let‑7a, miR‑21, miR‑26a and miR‑200a) and inflammation‑regulating miRNAs (miR‑29b, miR‑93, miR‑106b and miR‑100b). PGE2 induced significantly higher expression of the 3 anti‑apoptotic miRs, let‑7a, miR‑16a and miR‑200a, in LC when compared with the non‑treated control or E2. PGE2 significantly promoted a greater expression of let‑7a and miR‑26a in LC when compared with P4. Overall, PGE2 exerted the highest anti‑apoptotic and anti‑inflammatory effect in LC, which was comparable with E2. It was not observed among the inflammation‑regulating miRNAs in LC. PGF2α did not exert effects as prominent as those of PGE2. In MC, PGs and sex steroids exerted no similar effects on MC compared with LC. The present study demonstrated that PGE2 levels during menstruation may affect the growth of preexisting ULs without affecting the normal myometrium. Therefore, the control of secretion of PGs from the menstruating uterus or the administration of antagonists may be an alternative therapy for inhibiting the growth of ULs.

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