Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
August-2018 Volume 18 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
August-2018 Volume 18 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Antioxidants retard the ageing of mouse oocytes

  • Authors:
    • Yexing Xian
    • Lifeng Liang
    • Shutao Qi
    • Yingjun Xie
    • Bing Song
    • Shuming Ouyang
    • Yuhuan Xie
    • Xiaofang Sun
    • Weihua Wang
  • View Affiliations / Copyright

    Affiliations: Key Laboratory for Major Obstetrics Diseases of Guangdong Province, Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, The Third Hospital Affiliated of Guangzhou Medical University, Guangzhou, Guangdong 510150, P.R. China
    Copyright: © Xian et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1981-1986
    |
    Published online on: June 14, 2018
       https://doi.org/10.3892/mmr.2018.9167
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The aim of the present study was to verify the effects of heavy metal coupling agents (sodium citrate and EDTA) and antioxidants (acetyl carnitine and lipoic acid) on the number of oocytes, as well as the ageing of mitochondria, chromosomes and spindles in mice. C57BL/6 female mice were randomly classified into four groups (n=12 per group): i) Heavy metal coupling agent; ii) antioxidant; iii) mixed group; and iv) the normal control group. For the treatments, heavy metal coupling agents and antioxidants were added to the drinking water provided to the mice. Following 3, 6, 9 and 12 months of treatment, the number of oocytes and mitochondrial membrane potential were determined, and chromosome and spindle structures were observed. With increasing age, the experimental mice in the four groups showed significantly decreased numbers of oocytes, reduced mitochondrial activity, and increased rates of spindle and chromosome abnormalities, which indicated age‑induced ageing of mouse oocytes; thus, a mouse ageing oocyte model had been successfully established. For mice of the same age, more oocytes, higher mitochondrial activity, and lower spindle and chromosome malformation rates were detected in the antioxidant and mixed groups when compared with the normal control groups. Furthermore, no significant difference in the number of oocytes, mitochondrial activity or chromosome malformation rates was observed between the heavy metal coupling agent group and normal control group, which was possibly due to less metal being absorbed during the breeding process. Therefore, the results demonstrated that the antioxidants acetyl carnitine and lipoic acid may serve a role in delaying oocyte ageing.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Herrera LA, Prada D, Andonegui MA and Dueñas-González A: The epigenetic origin of aneuploidy. Curr Genomics. 9:43–50. 2008. View Article : Google Scholar : PubMed/NCBI

2 

Dulskiene V and Maroziene L: Impact on environmental factors on the reproductive system and fetal development. Medicina (Kaunas). 38:1072–1077. 2002.(In Lithuanian). PubMed/NCBI

3 

Montoya JM, Bernal A and Borrero C: Diagnostics in assisted human reproduction. Reprod Biomed Online. 5:198–210. 2002. View Article : Google Scholar : PubMed/NCBI

4 

Baart EB, Martini E, van den Berg I, Macklon NS, Galjaard RJ, Fauser BC and Van Opstal D: Preimplantation genetic screening reveals a high incidence of aneuploidy and mosaicism in embryos from young women undergoing IVF. Hum Reprod. 21:223–233. 2006. View Article : Google Scholar : PubMed/NCBI

5 

Jones KT: Meiosis in oocytes: Predisposition to aneuploidy and its increased incidence with age. Hum Reprod Update. 14:143–158. 2008. View Article : Google Scholar : PubMed/NCBI

6 

Chiang T, Schultz RM and Lampson MA: Meiotic origins of maternal age-related aneuploidy. Biol Reprod. 86:1–7. 2012. View Article : Google Scholar : PubMed/NCBI

7 

Zenzes MT: Smoking and reproduction: Gene damage to human gametes and embryos. Hum Reprod Update. 6:122–131. 2000. View Article : Google Scholar : PubMed/NCBI

8 

Kumar S: Occupational exposure associated with reproductive dysfunction. J Occup Health. 46:1–19. 2004. View Article : Google Scholar : PubMed/NCBI

9 

Gerhard I, Waibel S, Daniel V and Runnebaum B: Impact of heavy metals on hormonal and immunological factors in women with repeated miscarriages. Hum Reprod Update. 4:301–309. 1998. View Article : Google Scholar : PubMed/NCBI

10 

Tatone C, Amicarelli F, Carbone MC, Monteleone P, Caserta D, Marci R, Artini PG, Piomboni P and Focarelli R: Cellular and molecular aspects of ovarian follicle ageing. Hum Reprod Update. 14:131–142. 2008. View Article : Google Scholar : PubMed/NCBI

11 

Coccia ME and Rizzello F: Ovarian reserve. Ann N Y Acad Sci. 1127:27–30. 2008. View Article : Google Scholar : PubMed/NCBI

12 

Tarín JJ: Potential effects of age-associated oxidative stress on mammalian oocytes/embryos. Mol Hum Reprod. 2:717–724. 1996. View Article : Google Scholar : PubMed/NCBI

13 

Tarín JJ: Aetiology of age-associated aneuploidy: A mechanism based on the ‘free radical theory of ageing’. Hum Reprod. 10:1563–1565. 1995. View Article : Google Scholar : PubMed/NCBI

14 

Tamura H, Takasaki A, Miwa I, Taniguchi K, Maekawa R, Asada H, Taketani T, Matsuoka A, Yamagata Y, Shimamura K, et al: Oxidative stress impairs oocyte quality and melatonin protects oocytes from free radical damage and improves fertilization rate. J Pineal Res. 44:280–287. 2008. View Article : Google Scholar : PubMed/NCBI

15 

Chaube SK, Prasad PV, Thakur SC and Shrivastav TG: Hydrogen peroxide modulates meiotic cell cycle and induces morphological features characteristic of apoptosis in rat oocytes cultured in vitro. Apoptosis. 10:863–874. 2005. View Article : Google Scholar : PubMed/NCBI

16 

Zhang X, Wu XQ, Lu S, Guo YL and Ma X: Deficit of mitochondria-derived ATP during oxidative stress impairs mouse MII oocyte spindles. Cell Res. 16:841–850. 2006. View Article : Google Scholar : PubMed/NCBI

17 

Tarín JJ, Vendrell FJ, Ten J, Blanes R, van Blerkom J and Cano A: The oxidizing agent tertiary butyl hydroperoxide induces disturbances in spindle organization, c-meiosis, and aneuploidy in mouse oocytes. Mol Hum Reprod. 2:895–901. 1996. View Article : Google Scholar : PubMed/NCBI

18 

Liu L and Keefe DL: Ageing-associated aberration in meiosis of oocytes from senescence-accelerated mice. Hum Reprod. 17:2678–2685. 2002. View Article : Google Scholar : PubMed/NCBI

19 

Choi WJ, Banerjee J, Falcone T, Bena J, Agarwal A and Sharma RK: Oxidative stress and tumor necrosis factor-alpha-induced alterations in metaphase II mouse oocyte spindle structure. Fertil Steril. 88 4 Suppl:S1220–S1231. 2007. View Article : Google Scholar

20 

Tarín JJ, Gómez-Piquer V, Pertusa JF, Hermenegildo C and Cano A: Association of female aging with decreased parthenogenetic activation, raised MPF, and MAPKs activities and reduced levels of glutathione S-transferases activity and thiols in mouse oocytes. Mol Reprod Dev. 69:402–410. 2004. View Article : Google Scholar : PubMed/NCBI

21 

Bhattacharya S, Maheshwari A and Mollison J: Factors associated with failed treatment: An analysis of 121,744 women embarking on their first IVF cycles. PLoS One. 8:e822492013. View Article : Google Scholar : PubMed/NCBI

22 

Tarín JJ, Pérez-Albalá S and Cano A: Oral antioxidants counteract the negative effects of female aging on oocyte quantity and quality in the mouse. Mol Reprod Dev. 61:385–397. 2002. View Article : Google Scholar : PubMed/NCBI

23 

Tarín JJ, Vendrell FJ, Ten J and Cano A: Antioxidant therapy counteracts the disturbing effects of diamide and maternal ageing on meiotic division and chromosomal segregation in mouse oocytes. Mol Hum Reprod. 4:281–288. 1998. View Article : Google Scholar : PubMed/NCBI

24 

Liu J, Liu M, Ye X, Liu K, Huang J, Wang L, Ji G, Liu N, Tang X, Baltz JM, et al: Delay in oocyte aging in mice by the antioxidant N-acetyl-L-cysteine (NAC). Hum Reprod. 27:1411–1420. 2012. View Article : Google Scholar : PubMed/NCBI

25 

Harman D: The free radical theory of aging: Effect of age on serum copper levels. J Gerontol. 20:151–153. 1965. View Article : Google Scholar : PubMed/NCBI

26 

Harman D: The biologic clock: The mitochondria? J Am Geriatr Soc. 20:145–147. 1972. View Article : Google Scholar : PubMed/NCBI

27 

Takahashi T, Takahashi E, Igarashi H, Tezuka N and Kurachi H: Impact of oxidative stress in aged mouse oocytes on calcium oscillations at fertilization. Mol Reprod Dev. 66:143–152. 2003. View Article : Google Scholar : PubMed/NCBI

28 

Liu L, Trimarchi JR and Keefe DL: Involvement of mitochondria in oxidative stress-induced cell death in mouse zygotes. Biol Reprod. 62:1745–1753. 2000. View Article : Google Scholar : PubMed/NCBI

29 

Zhang D, Keilty D, Zhang ZF and Chian RC: Mitochondria in oocyte aging: Current understanding. Facts Views Vis Obgyn. 9:29–38. 2017.PubMed/NCBI

30 

Wang WH and Sun QY: Meiotic spindle, spindle checkpoint and embryonic aneuploidy. Front Biosci. 11:620–636. 2006. View Article : Google Scholar : PubMed/NCBI

31 

Vogt E, Kirsch-Volders M, Parry J and Eichenlaub-Ritter U: Spindle formation, chromosome segregation and the spindle checkpoint in mammalian oocytes and susceptibility to meiotic error. Mutat Res. 651:14–29. 2008. View Article : Google Scholar : PubMed/NCBI

32 

Hassold T and Hunt P: To err (meiotically) is human: The genesis of human aneuploidy. Nat Rev Genet. 2:280–291. 2001. View Article : Google Scholar : PubMed/NCBI

33 

Hassold T, Hall H and Hunt P: The origin of human aneuploidy: Where we have been, where we are going. Hum Mol Genet 16 Spec No. 2:R203–R208. 2007. View Article : Google Scholar

34 

Djahanbakhch O, Ezzati M and Zosmer A: Reproductive ageing in women. J Pathol. 211:219–231. 2007. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Xian Y, Liang L, Qi S, Xie Y, Song B, Ouyang S, Xie Y, Sun X and Wang W: Antioxidants retard the ageing of mouse oocytes. Mol Med Rep 18: 1981-1986, 2018.
APA
Xian, Y., Liang, L., Qi, S., Xie, Y., Song, B., Ouyang, S. ... Wang, W. (2018). Antioxidants retard the ageing of mouse oocytes. Molecular Medicine Reports, 18, 1981-1986. https://doi.org/10.3892/mmr.2018.9167
MLA
Xian, Y., Liang, L., Qi, S., Xie, Y., Song, B., Ouyang, S., Xie, Y., Sun, X., Wang, W."Antioxidants retard the ageing of mouse oocytes". Molecular Medicine Reports 18.2 (2018): 1981-1986.
Chicago
Xian, Y., Liang, L., Qi, S., Xie, Y., Song, B., Ouyang, S., Xie, Y., Sun, X., Wang, W."Antioxidants retard the ageing of mouse oocytes". Molecular Medicine Reports 18, no. 2 (2018): 1981-1986. https://doi.org/10.3892/mmr.2018.9167
Copy and paste a formatted citation
x
Spandidos Publications style
Xian Y, Liang L, Qi S, Xie Y, Song B, Ouyang S, Xie Y, Sun X and Wang W: Antioxidants retard the ageing of mouse oocytes. Mol Med Rep 18: 1981-1986, 2018.
APA
Xian, Y., Liang, L., Qi, S., Xie, Y., Song, B., Ouyang, S. ... Wang, W. (2018). Antioxidants retard the ageing of mouse oocytes. Molecular Medicine Reports, 18, 1981-1986. https://doi.org/10.3892/mmr.2018.9167
MLA
Xian, Y., Liang, L., Qi, S., Xie, Y., Song, B., Ouyang, S., Xie, Y., Sun, X., Wang, W."Antioxidants retard the ageing of mouse oocytes". Molecular Medicine Reports 18.2 (2018): 1981-1986.
Chicago
Xian, Y., Liang, L., Qi, S., Xie, Y., Song, B., Ouyang, S., Xie, Y., Sun, X., Wang, W."Antioxidants retard the ageing of mouse oocytes". Molecular Medicine Reports 18, no. 2 (2018): 1981-1986. https://doi.org/10.3892/mmr.2018.9167
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team