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Characteristics and multi‑lineage differentiation of bone marrow mesenchymal stem cells derived from the Tibetan mastiff

  • Authors:
    • Shuang Zhang
    • Chenqiong Zhao
    • Shi Liu
    • Yufeng Wang
    • Yuhua Zhao
    • Weijun Guan
    • Zhiqiang Zhu
  • View Affiliations / Copyright

    Affiliations: Scientific Research Center, Harbin Sport University, Harbin, Heilongjiang 150008, P.R. China, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2097-2109
    |
    Published online on: June 15, 2018
       https://doi.org/10.3892/mmr.2018.9172
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Abstract

Bone marrow mesenchymal stem cells (BM‑MSCs) are pluripotent stem cells that are regarded as ideal resources for the reconstruction of tissues and organs. The Tibetan mastiff is a breed of domesticated Chinese native dog that is well‑adjusted to the high‑altitude environments of Tibet. To the best of our knowledge, the biological characterization and multi‑lineage differentiation of Tibetan mastiff BM‑MSCs have not been reported previously. Therefore, the present study aimed to investigate the biological characteristics and therapeutic potential of Tibetan mastiff BM‑MSCs. A cell culture system was constructed and cells were cultured to 23 passages in vitro. Growth curves and colony formation studies suggested that BM‑MSCs had a high self‑renewal capacity and that their proliferation rate declined with age. Karyotype analysis demonstrated that BM‑MSCs were diploid and genetically stable. Semi‑quantitative polymerase chain reaction analysis revealed that BM‑MSCs positively expressed cluster of differentiation (CD)73, CD90, CD105, CD166 and vimentin, although they were negative for the endothelial cell marker CD31. Additionally, immunofluorescence staining revealed that the cells expressed CD29, CD44, CD90, CD105 and vimentin. Flow cytometric analysis revealed that the rates of positive expression of vimentin, CD44, CD90 and CD105 were all >97%. BM‑MSCs were able to differentiate into adipocytes, osteoblasts, cartilage cells, hepatocytes and functional insulin‑secreting cells. In conclusion, Tibetan mastiff BM‑MSCs may be purified successfully using a whole bone marrow culture method. The findings of the current study suggested important potential applications of BM‑MSCs as a source for regenerative therapies.
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View References

1 

Cristancho AG and Lazar MA: Forming functional fat: A growing understanding of adipocyte differentiation. Nat Rev Mol Cell Biol. 12:722–734. 2011. View Article : Google Scholar : PubMed/NCBI

2 

De Coppi P, Bartsch G Jr, Siddiqui MM, Xu T, Santos CC, Perin L, Mostoslavsky G, Serre AC, Snyder EY, Yoo JJ, et al: Isolation of amniotic stem cell lines with potential for therapy. Nat Biotechnol. 25:100–106. 2007. View Article : Google Scholar : PubMed/NCBI

3 

Pozzobon M, Piccoli M, Schiavo AA, Atala A and De Coppi P: Isolation of c-Kit+ human amniotic fluid stem cells from second trimester. Methods Mol Biol. 1035:191–198. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Kinnaird T, Stabile E, Burnett MS, Lee CW, Barr S, Fuchs S and Epstein SE: Marrow-derived stromal cells express genes encoding a broad spectrum of arteriogenic cytokines and promote in vitro and in vivo arteriogenesis through paracrine mechanisms. Circ Res. 94:678–685. 2004. View Article : Google Scholar : PubMed/NCBI

5 

da Silva Meirelles L, Caplan AI and Nardi NB: In search of the in vivo identity of mesenchymal stem cells. Stem Cells. 26:2287–2299. 2008. View Article : Google Scholar : PubMed/NCBI

6 

Nardi Beyer N and da Silva Meirelles L: Mesenchymal stem cells: Isolation, in vitro expansion and characterization. Handb Exp Pharmacol. 249–282. 2006. View Article : Google Scholar

7 

Deans RJ and Moseley AB: Mesenchymal stem cells: Biology and potential clinical uses. Exp Hematol. 28:875–884. 2000. View Article : Google Scholar : PubMed/NCBI

8 

Sanchez-Ramos J, Song S, Cardozo-Pelaez F, Hazzi C, Stedeford T, Willing A, Freeman TB, Saporta S, Janssen W, Patel N, et al: Adult bone marrow stromal cells differentiate into neural cells in vitro. Exp Neurol. 164:247–256. 2000. View Article : Google Scholar : PubMed/NCBI

9 

McLean K, Gong Y, Choi Y, Deng N, Yang K, Bai S, Cabrera L, Keller E, McCauley L, Cho KR and Buckanovich RJ: Human ovarian carcinoma-associated mesenchymal stem cells regulate cancer stem cells and tumorigenesis via altered BMP production. J Clin Invest. 121:3206–3219. 2011. View Article : Google Scholar : PubMed/NCBI

10 

Yang X, Hou J, Han Z, Wang Y, Hao C, Wei L and Shi Y: One cell, multiple roles: Contribution of mesenchymal stem cells to tumor development in tumor microenvironment. Cell Biosci. 3:52013. View Article : Google Scholar : PubMed/NCBI

11 

Chen S, Wang L, Fan J, Ye C, Dominguez D, Zhang Y, Curiel TJ, Fang D, Kuzel TM and Zhang B: Host miR155 promotes tumor growth through a myeloid-derived suppressor cell-dependent mechanism. Cancer Res. 75:519–531. 2015. View Article : Google Scholar : PubMed/NCBI

12 

Wang J, Yu F, Jia X, Iwanowycz S, Wang Y, Huang S, Ai W and Fan D: MicroRNA-155 deficiency enhances the recruitment and functions of myeloid-derived suppressor cells in tumor microenvironment and promotes solid tumor growth. Int J Cancer. 136:E602–E613. 2015. View Article : Google Scholar : PubMed/NCBI

13 

Ward CL, Sanchez CJ Jr, Pollot BE, Romano DR, Hardy SK, Becerra SC, Rathbone CR and Wenke JC: Soluble factors from biofilms of wound pathogens modulate human bone marrow-derived stromal cell differentiation, migration, angiogenesis, and cytokine secretion. BMC Microbiol. 15:752015. View Article : Google Scholar : PubMed/NCBI

14 

Zhang D, Liu X, Peng J, He D, Lin T, Zhu J, Li X, Zhang Y and Wei G: Potential spermatogenesis recovery with bone marrow mesenchymal stem cells in an azoospermic rat model. Int J Mol Sci. 15:13151–13165. 2014. View Article : Google Scholar : PubMed/NCBI

15 

Tan SL, Ahmad TS, Selvaratnam L and Kamarul T: Isolation, characterization and the multi-lineage differentiation potential of rabbit bone marrow-derived mesenchymal stem cells. J Anat. 222:437–450. 2013. View Article : Google Scholar : PubMed/NCBI

16 

Adamzyk C, Kachel P, Hoss M, Gremse F, Modabber A, Holzle F, Tolba R, Neuss S and Lethaus B: Bone tissue engineering using polyetherketoneketone scaffolds combined with autologous mesenchymal stem cells in a sheep calvarial defect model. J Craniomaxillofac Surg. 44:985–994. 2016. View Article : Google Scholar : PubMed/NCBI

17 

Kocamaz E, Gok D, Cetinkaya A and Tufan AC: Implication of C-type natriuretic peptide-3 signaling in glycosaminoglycan synthesis and chondrocyte hypertrophy during TGF-beta1 induced chondrogenic differentiation of chicken bone marrow-derived mesenchymal stem cells. J Mol Histol. 43:497–508. 2012. View Article : Google Scholar : PubMed/NCBI

18 

Ramírez-Espinosa JJ, González-Dávalos L, Shimada A, Piña E, Varela-Echavarria A and Mora O: Bovine (bos taurus) bone marrow mesenchymal cell differentiation to adipogenic and myogenic lineages. Cells Tissues Organs. 201:51–64. 2016. View Article : Google Scholar : PubMed/NCBI

19 

Wang M, Wang YH, Ye Q, Meng P, Yin H and Zhang DL: Serological survey of toxoplasma gondii in tibetan mastiffs (canis lupus familiaris) and yaks (Bos grunniens) in Qinghai, China. Parasit Vectors. 5:352012. View Article : Google Scholar : PubMed/NCBI

20 

Messerschmidt GL, Bowles C, Alsaker R, McCormack K, Corbitt RH, Mosley KR and Deisseroth AB: Prognostic indicators of tumor response to Staphylococcus aureus Cowan strain I plasma perfusion. J Natl Cancer Inst. 71:535–538. 1983.PubMed/NCBI

21 

Cui Y, Guo W, Li D, Wang L, Shi CX, Brookmeyer R, Detels R, Ge L, Ding Z and Wu Z: Estimating HIV incidence among key affected populations in China from serial cross-sectional surveys in 2010–2014. J Int AIDS Soc. 19:206092016. View Article : Google Scholar : PubMed/NCBI

22 

Li Q, Liu Z, Li Y, Zhao X, Dong L, Pan Z, Sun Y, Li N, Xu Y and Xie Z: Origin and phylogenetic analysis of tibetan mastiff based on the mitochondrial DNA sequence. J Genet Genomics. 35:335–340. 2008. View Article : Google Scholar : PubMed/NCBI

23 

Kijas JW, Miller BJ, Pearce-Kelling SE, Aguirre GD and Acland GM: Canine models of ocular disease: Outcross breedings define a dominant disorder present in the English mastiff and bull mastiff dog breeds. J Hered. 94:27–30. 2003. View Article : Google Scholar : PubMed/NCBI

24 

Huang B, Cheng X, Wang H, Huang W, la Ga Hu Z, Wang D, Zhang K, Zhang H, Xue Z and Da Y: Mesenchymal stem cells and their secreted molecules predominantly ameliorate fulminant hepatic failure and chronic liver fibrosis in mice respectively. J Transl Med. 14:452016. View Article : Google Scholar : PubMed/NCBI

25 

Chouinard L, Martineau D, Forget C and Girard C: Use of polymerase chain reaction and immunohistochemistry for detection of canine adenovirus type 1 in formalin-fixed, paraffin-embedded liver of dogs with chronic hepatitis or cirrhosis. J Vet Diagn Invest. 10:320–325. 1998. View Article : Google Scholar : PubMed/NCBI

26 

Nagamoto Y, Takayama K, Ohashi K, Okamoto R, Sakurai F, Tachibana M, Kawabata K and Mizuguchi H: Transplantation of a human iPSC-derived hepatocyte sheet increases survival in mice with acute liver failure. J Hepatol. 64:1068–1075. 2016. View Article : Google Scholar : PubMed/NCBI

27 

Li LH, Zhao YY and Wu XG: Progress in the study of diabetes in dogs. China Vet J. 8:64–65. 2008.

28 

Samarghandian S, Azimi-Nezhad M, Samini F and Farkhondeh T: Chrysin treatment improves diabetes and its complications in liver, brain, and pancreas in streptozotocin-induced diabetic rats. Can J Physiol Pharmacol. 94:388–393. 2016. View Article : Google Scholar : PubMed/NCBI

29 

Chaiyasut C, Kusirisin W, Lailerd N, Lerttrakarnnon P, Suttajit M and Srichairatanakool S: Effects of phenolic compounds of fermented thai indigenous plants on oxidative stress in streptozotocin-induced diabetic rats. Evid Based Complement Alternat Med. 2011:7493072011. View Article : Google Scholar : PubMed/NCBI

30 

Motta-Silva AC, Aleva NA, Chavasco JK, Armond MC, Franca JP and Pereira LJ: Erythematous oral candidiasis in patients with controlled type II diabetes mellitus and complete dentures. Mycopathologia. 169:215–223. 2010. View Article : Google Scholar : PubMed/NCBI

31 

Bai C, Chen S, Gao Y, Shan Z, Guan W and Ma Y: Multi-lineage potential research of bone marrow mesenchymal stem cells from Bama miniature pig. J Exp Zool B Mol Dev Evol. 324:671–685. 2015. View Article : Google Scholar : PubMed/NCBI

32 

Anjos-Afonso F and Bonnet D: Isolation, culture, and differentiation potential of mouse marrow stromal cells. Curr Protoc Stem Cell Biol Chapter. 2:Unit 2B.3. 2008. View Article : Google Scholar

33 

Majumdar MK, Banks V, Peluso DP and Morris EA: Isolation, characterization, and chondrogenic potential of human bone marrow-derived multipotential stromal cells. J Cell Physiol. 185:98–106. 2000. View Article : Google Scholar : PubMed/NCBI

34 

Reyes M, Lund T, Lenvik T, Aguiar D, Koodie L and Verfaillie CM: Purification and ex vivo expansion of postnatal human marrow mesodermal progenitor cells. Blood. 98:2615–2625. 2001. View Article : Google Scholar : PubMed/NCBI

35 

Zhang S, Bai CY, Ma YH, Li XC, Gao YH, Fan YN, Wei JG and Zheng D: The characterisation and functional β-cell differentiation of duck pancreas-derived mesenchymal cells. Br Poult Sci. 57:201–210. 2016. View Article : Google Scholar : PubMed/NCBI

36 

Lv FJ, Tuan RS, Cheung KM and Leung VY: Concise review: The surface markers and identity of human mesenchymal stem cells. Stem Cells. 32:1408–1419. 2014. View Article : Google Scholar : PubMed/NCBI

37 

Noort WA, Oerlemans MI, Rozemuller H, Feyen D, Jaksani S, Stecher D, Naaijkens B, Martens AC, Bühring HJ, Doevendans PA and Sluijter JP: Human versus porcine mesenchymal stromal cells: Phenotype, differentiation potential, immunomodulation and cardiac improvement after transplantation. J Cell Mol Med. 2012.16:1827–1839. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhang S, Zhao C, Liu S, Wang Y, Zhao Y, Guan W and Zhu Z: Characteristics and multi‑lineage differentiation of bone marrow mesenchymal stem cells derived from the Tibetan mastiff. Mol Med Rep 18: 2097-2109, 2018.
APA
Zhang, S., Zhao, C., Liu, S., Wang, Y., Zhao, Y., Guan, W., & Zhu, Z. (2018). Characteristics and multi‑lineage differentiation of bone marrow mesenchymal stem cells derived from the Tibetan mastiff. Molecular Medicine Reports, 18, 2097-2109. https://doi.org/10.3892/mmr.2018.9172
MLA
Zhang, S., Zhao, C., Liu, S., Wang, Y., Zhao, Y., Guan, W., Zhu, Z."Characteristics and multi‑lineage differentiation of bone marrow mesenchymal stem cells derived from the Tibetan mastiff". Molecular Medicine Reports 18.2 (2018): 2097-2109.
Chicago
Zhang, S., Zhao, C., Liu, S., Wang, Y., Zhao, Y., Guan, W., Zhu, Z."Characteristics and multi‑lineage differentiation of bone marrow mesenchymal stem cells derived from the Tibetan mastiff". Molecular Medicine Reports 18, no. 2 (2018): 2097-2109. https://doi.org/10.3892/mmr.2018.9172
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang S, Zhao C, Liu S, Wang Y, Zhao Y, Guan W and Zhu Z: Characteristics and multi‑lineage differentiation of bone marrow mesenchymal stem cells derived from the Tibetan mastiff. Mol Med Rep 18: 2097-2109, 2018.
APA
Zhang, S., Zhao, C., Liu, S., Wang, Y., Zhao, Y., Guan, W., & Zhu, Z. (2018). Characteristics and multi‑lineage differentiation of bone marrow mesenchymal stem cells derived from the Tibetan mastiff. Molecular Medicine Reports, 18, 2097-2109. https://doi.org/10.3892/mmr.2018.9172
MLA
Zhang, S., Zhao, C., Liu, S., Wang, Y., Zhao, Y., Guan, W., Zhu, Z."Characteristics and multi‑lineage differentiation of bone marrow mesenchymal stem cells derived from the Tibetan mastiff". Molecular Medicine Reports 18.2 (2018): 2097-2109.
Chicago
Zhang, S., Zhao, C., Liu, S., Wang, Y., Zhao, Y., Guan, W., Zhu, Z."Characteristics and multi‑lineage differentiation of bone marrow mesenchymal stem cells derived from the Tibetan mastiff". Molecular Medicine Reports 18, no. 2 (2018): 2097-2109. https://doi.org/10.3892/mmr.2018.9172
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