miR‑590‑5p suppresses osteosarcoma cell proliferation and invasion via targeting KLF5

Cai,Wei; Xu,Yong; Yin,Jian; Zuo,Wenshan; Su,Zhen

doi:10.3892/mmr.2018.9173

miR‑590‑5p suppresses osteosarcoma cell proliferation and invasion via targeting KLF5

Authors:
- Wei Cai
- Yong Xu
- Jian Yin
- Wenshan Zuo
- Zhen Su
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Affiliations: Orthopedics Department, The Affiliated Huaian No. 1 People's Hospital of Nanjing Medical University, Huai'an, Jiangsu 223300, P.R. China, Orthopedics Department, The Affiliated Jiangning Hospital, Nanjing Medical University, Nanjing, Jiangsu 211100, P.R. China, Anesthesiology Department, The Affiliated Huaian No. 1 People's Hospital of Nanjing Medical University, Huai'an, Jiangsu 223300, P.R. China
Published online on: June 15, 2018 https://doi.org/10.3892/mmr.2018.9173
Pages: 2328-2334

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Abstract

Recently, microRNA (miR)‑590‑5p has been shown to inhibit tumorigenesis in colorectal and breast cancer; however, its function in osteosarcoma (OS) requires further investigation. In the present study miR‑590‑5p expression was poorly expressed in OS samples and cell lines when compared with that observed in normal cells. In addition, overexpression of miR‑590‑5p significantly reduced the proliferation, migration and invasion of SAOS2 and U2OS cells in vitro, as well as inhibiting tumor sizes in vivo. The results revealed that miR‑590‑5p directly targeted Kruppel‑like factor 5 (KLF5) in SAOS2 and U2OS cells. Their expression was inversely correlated with OS tissues. Finally, it was demonstrated that overexpression of KLF5 rescued the inhibitory effects of miR‑590‑5p on cell proliferation, migration and invasion. Overall, the results of the present study suggested that the miR‑590‑5p/KLF5 axis may regulate OS progression and thus, may be a novel therapeutic target for the treatment of patients with OS.

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