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Article

PEP‑1‑glutaredoxin 1 protects against hippocampal neuronal cell damage from oxidative stress via regulation of MAPK and apoptotic signaling pathways

  • Authors:
    • Eun Ji Ryu
    • Dae Won Kim
    • Min Jea Shin
    • Hyo Sang Jo
    • Jung Hwan Park
    • Su Bin Cho
    • Chi Hern Lee
    • Hyeon Ji Yeo
    • Eun Ji Yeo
    • Yeon Joo Choi
    • Duk‑Soo Kim
    • Sung‑Woo Cho
    • Yong‑Jun Cho
    • Eun Jeong Sohn
    • Ora Son
    • Keun Wook Lee
    • Kyu Hyung Han
    • Jinseu Park
    • Won Sik Eum
    • Soo Young Choi
  • View Affiliations / Copyright

    Affiliations: Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University, Chuncheon, Gangwon 24252, Republic of South Korea, Department of Biochemistry and Molecular Biology, Research Institute of Oral Sciences, College of Dentistry, Gangneung‑Wonju National University, Gangneung, Gangwon 25457, Republic of South Korea, Department of Anatomy, College of Medicine, Soonchunhyang University, Cheonan‑Si, South Chungcheong 31538, Republic of South Korea, Department of Biochemistry and Molecular Biology, University of Ulsan College of Medicine, Seoul 05505, Republic of South Korea, Department of Neurosurgery, Hallym University Medical Center, Chuncheon, Gangwon 24253, Republic of South Korea
  • Pages: 2216-2228
    |
    Published online on: June 15, 2018
       https://doi.org/10.3892/mmr.2018.9176
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Abstract

Oxidative stress is known to be a primary risk factor for neuronal diseases. Glutaredoxin (GLRX)‑1, a redox‑regulator of the thioredoxin superfamily, is known to exhibit an important role in cell survival via various cellular functions. However, the precise roles of GLRX1 in brain ischemia are still not fully understood. The present study investigated whether transduced PEP‑1‑GLRX1 protein has protective effects against oxidative stress in cells and in an animal model. Transduced PEP‑1‑GLRX1 protein increased HT‑22 cell viability under oxidative stress and this fusion protein significantly reduced intracellular reactive oxygen species and levels of DNA damage. In addition, PEP‑1‑GLRX1 protein regulated RAC‑a serine/threonine‑protein kinase and mitogen‑activated protein kinase signaling, in addition to apoptotic signaling including B cell lymphoma (Bcl)‑2, Bcl‑2 associated X, apoptosis regulator, pro‑caspase‑9 and p53 expression levels. In an ischemic animal model, it was verified that PEP‑1‑GLRX1 transduced into the Cornu Ammonis 1 region of the animal brain, where it markedly protected against ischemic injury. These results indicate that PEP‑1‑GLRX1 attenuates neuronal cell death resulting from oxidative stress in vitro and in vivo. Therefore, PEP‑1‑GLRX1 may exhibit a beneficial role in the treatment of neuronal disorders, including ischemic injury.
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Copy and paste a formatted citation
Spandidos Publications style
Ryu EJ, Kim DW, Shin MJ, Jo HS, Park JH, Cho SB, Lee CH, Yeo HJ, Yeo EJ, Choi YJ, Choi YJ, et al: PEP‑1‑glutaredoxin 1 protects against hippocampal neuronal cell damage from oxidative stress via regulation of MAPK and apoptotic signaling pathways. Mol Med Rep 18: 2216-2228, 2018.
APA
Ryu, E.J., Kim, D.W., Shin, M.J., Jo, H.S., Park, J.H., Cho, S.B. ... Choi, S.Y. (2018). PEP‑1‑glutaredoxin 1 protects against hippocampal neuronal cell damage from oxidative stress via regulation of MAPK and apoptotic signaling pathways. Molecular Medicine Reports, 18, 2216-2228. https://doi.org/10.3892/mmr.2018.9176
MLA
Ryu, E. J., Kim, D. W., Shin, M. J., Jo, H. S., Park, J. H., Cho, S. B., Lee, C. H., Yeo, H. J., Yeo, E. J., Choi, Y. J., Kim, D., Cho, S., Cho, Y., Sohn, E. J., Son, O., Lee, K. W., Han, K. H., Park, J., Eum, W. S., Choi, S. Y."PEP‑1‑glutaredoxin 1 protects against hippocampal neuronal cell damage from oxidative stress via regulation of MAPK and apoptotic signaling pathways". Molecular Medicine Reports 18.2 (2018): 2216-2228.
Chicago
Ryu, E. J., Kim, D. W., Shin, M. J., Jo, H. S., Park, J. H., Cho, S. B., Lee, C. H., Yeo, H. J., Yeo, E. J., Choi, Y. J., Kim, D., Cho, S., Cho, Y., Sohn, E. J., Son, O., Lee, K. W., Han, K. H., Park, J., Eum, W. S., Choi, S. Y."PEP‑1‑glutaredoxin 1 protects against hippocampal neuronal cell damage from oxidative stress via regulation of MAPK and apoptotic signaling pathways". Molecular Medicine Reports 18, no. 2 (2018): 2216-2228. https://doi.org/10.3892/mmr.2018.9176
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Spandidos Publications style
Ryu EJ, Kim DW, Shin MJ, Jo HS, Park JH, Cho SB, Lee CH, Yeo HJ, Yeo EJ, Choi YJ, Choi YJ, et al: PEP‑1‑glutaredoxin 1 protects against hippocampal neuronal cell damage from oxidative stress via regulation of MAPK and apoptotic signaling pathways. Mol Med Rep 18: 2216-2228, 2018.
APA
Ryu, E.J., Kim, D.W., Shin, M.J., Jo, H.S., Park, J.H., Cho, S.B. ... Choi, S.Y. (2018). PEP‑1‑glutaredoxin 1 protects against hippocampal neuronal cell damage from oxidative stress via regulation of MAPK and apoptotic signaling pathways. Molecular Medicine Reports, 18, 2216-2228. https://doi.org/10.3892/mmr.2018.9176
MLA
Ryu, E. J., Kim, D. W., Shin, M. J., Jo, H. S., Park, J. H., Cho, S. B., Lee, C. H., Yeo, H. J., Yeo, E. J., Choi, Y. J., Kim, D., Cho, S., Cho, Y., Sohn, E. J., Son, O., Lee, K. W., Han, K. H., Park, J., Eum, W. S., Choi, S. Y."PEP‑1‑glutaredoxin 1 protects against hippocampal neuronal cell damage from oxidative stress via regulation of MAPK and apoptotic signaling pathways". Molecular Medicine Reports 18.2 (2018): 2216-2228.
Chicago
Ryu, E. J., Kim, D. W., Shin, M. J., Jo, H. S., Park, J. H., Cho, S. B., Lee, C. H., Yeo, H. J., Yeo, E. J., Choi, Y. J., Kim, D., Cho, S., Cho, Y., Sohn, E. J., Son, O., Lee, K. W., Han, K. H., Park, J., Eum, W. S., Choi, S. Y."PEP‑1‑glutaredoxin 1 protects against hippocampal neuronal cell damage from oxidative stress via regulation of MAPK and apoptotic signaling pathways". Molecular Medicine Reports 18, no. 2 (2018): 2216-2228. https://doi.org/10.3892/mmr.2018.9176
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